Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-fructose 1,6-diphosphate | activates | Homo sapiens | |
D-fructose 2,6-diphosphate | activates | Homo sapiens | |
D-fructose 6-phosphate | potent activator of the unphosphorylated recombinant enzyme, half-maximal activation at 0.16 mM | Homo sapiens | |
D-glucose 1-phosphate | activates | Homo sapiens | |
D-glucose 6-phosphate | activates | Homo sapiens | |
D-ribulose 5-phosphate | activates | Homo sapiens | |
D-xylulose 5-phosphate | activates | Homo sapiens | |
phosphoenolpyruvate | activates | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
expression in Escherichia coli | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Rattus norvegicus | |
additional information | - |
additional information | Km-value for phosphorylated enzyme forms | Homo sapiens | |
2.59 | - |
CoA | - |
Homo sapiens | |
41 | - |
ATP | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
120000 | - |
x * 120000, SDS-PAGE | Homo sapiens |
480000 | - |
velocity sedimentation | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | phosphorylation of recombinant human ATP:citrate lyase by cAMP-dependent protein kinase abolishes homotropic allosteric regulation of the enzyme by citrate and increases the enzyme activity. Cyclic AMP-dependent protein kinase catalyzes the incorporation of 1 mol of phosphate per mol of enzyme homotetramer, and glycogen synthase kinase-3 incorporated an additional 2 mol of phosphate into the phosphorylated protein | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2 | - |
recombinant enzyme | Homo sapiens |
Storage Stability | Organism |
---|---|
-20°C, 1 year, stable | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + citrate + CoA | - |
Homo sapiens | ADP + phosphate + acetyl-CoA + oxaloacetate | - |
? | |
ATP + citrate + CoA | - |
Rattus norvegicus | ADP + phosphate + acetyl-CoA + oxaloacetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | - |
Rattus norvegicus |
? | x * 120000, SDS-PAGE | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
4 h, stable | Homo sapiens |