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Literature summary for 2.3.1.9 extracted from

  • Hou, J.; Feng, B.; Han, J.; Liu, H.; Zhao, D.; Zhou, J.; Xiang, H.
    Haloarchaeal-type beta-ketothiolases involved in poly(3-hydroxybutyrate-co-3-hydroxyvalerate) synthesis in Haloferax mediterranei (2013), Appl. Environ. Microbiol., 79, 5104-5111.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.9

Application

Application Comment Organism
synthesis the enzyme has biotechnological potential in haloarchaea for production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with controllable 3-oxopentano content, from unrelated cheap carbon sources Haloferax mediterranei

Protein Variants

Protein Variants Comment Organism
additional information the deletion mutant strain expressing the enzyme with an S83A, H281Q, or H331Q mutation in BktBalpha does not accumulate polyhydroxyalkanoate at all, whereas strains expressing the enzyme with a C365A or C365S mutation in subunit BktBalpha have no significant effect on poly(3-hydroxybutyrate-co-3-hydroxyvalerate) accumulation Haloferax mediterranei
additional information the introduction of the S84A, H282Q, or H332Q mutation into subunit PhaAalpha results in inactive enzyme, while the C366A or C366S mutation does not obviously affect PhaA activity Haloferax mediterranei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 acetyl-CoA Haloferax mediterranei the enzyme is responsible for supplying the precursors for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) CoA + acetoacetyl-CoA
-
 ?
2 acetyl-CoA Haloferax mediterranei DSM 1411 the enzyme is responsible for supplying the precursors for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) CoA + acetoacetyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Haloferax mediterranei I3R3D1 and I3R3D0 I3R3D1: catalytic subunit PhaAalpha, I3RA71: subunit PhaAbeta
-
Haloferax mediterranei I3RA72 and I3RA71 I3RA72: catalytic subunit BktBalpha, I3RA71: subunit BktBbeta
-
Haloferax mediterranei DSM 1411 I3R3D1 and I3R3D0 I3R3D1: catalytic subunit PhaAalpha, I3RA71: subunit PhaAbeta
-
Haloferax mediterranei DSM 1411 I3RA72 and I3RA71 I3RA72: catalytic subunit BktBalpha, I3RA71: subunit BktBbeta
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 acetyl-CoA
-
 Haloferax mediterranei CoA + acetoacetyl-CoA
-
 ?
2 acetyl-CoA the enzyme is responsible for supplying the precursors for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Haloferax mediterranei CoA + acetoacetyl-CoA
-
 ?
2 acetyl-CoA
-
 Haloferax mediterranei DSM 1411 CoA + acetoacetyl-CoA
-
 ?
2 acetyl-CoA the enzyme is responsible for supplying the precursors for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Haloferax mediterranei DSM 1411 CoA + acetoacetyl-CoA
-
 ?
acetyl-CoA + propionyl-CoA
-
 Haloferax mediterranei CoA + 3-oxopentanoyl-CoA
-
 ?
acetyl-CoA + propionyl-CoA
-
 Haloferax mediterranei DSM 1411 CoA + 3-oxopentanoyl-CoA
-
 ?
additional information the catalytic triad of BktBalpha is likely to be Ser-His-His Haloferax mediterranei ?
-
 ?
additional information the catalytic triad of PhaAalpha is likely to be Ser-His-His Haloferax mediterranei ?
-
 ?
additional information the catalytic triad of PhaAalpha is likely to be Ser-His-His Haloferax mediterranei DSM 1411 ?
-
 ?
additional information the catalytic triad of BktBalpha is likely to be Ser-His-His Haloferax mediterranei DSM 1411 ?
-
 ?

Subunits

Subunits Comment Organism
? composed of two different types of subunits: catalytic subunit PhaAalpha, and subunit PhaABbeta Haloferax mediterranei
More composed of two different types of subunits: catalytic subunit BktBalpha, and BktBbeta Haloferax mediterranei

Synonyms

Synonyms Comment Organism
HFX_1022 locus name, subunit PhaAbeta Haloferax mediterranei
HFX_1023 locus name, subunit PhaAalpha Haloferax mediterranei
HFX_6003 locus name, subunit BktBbeta Haloferax mediterranei
HFX_6004 locus name, subunit BktBalpha Haloferax mediterranei

General Information

General Information Comment Organism
physiological function the enzyme is responsible for supplying the precursors for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Haloferax mediterranei