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Literature summary for 2.3.1.75 extracted from

  • Roettig, A.; Strittmatter, C.S.; Schauer, J.; Hiessl, S.; Poehlein, A.; Daniel, R.; Steinbuechel, A.
    Role of wax ester synthase/acyl coenzyme A diacylglycerol acyltransferase in oleaginous Streptomyces sp. strain G25 (2016), Appl. Environ. Microbiol., 82, 5969-5981 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.75

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C41(DE3) cells Streptomyces sp. G25
gene atfG25, DNA and amino acid sequence determination and analysis, genomic structure, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain C41(DE3) Streptomyces sp. G25

Protein Variants

Protein Variants Comment Organism
additional information generation of an atfG25OMEGAApr insertion mutant of Streptomyces sp. G25 Streptomyces sp. G25

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-CoA + a long-chain alcohol Streptomyces sp. G25
-
 CoA + a long-chain ester
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces sp. G25
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Superdex S200 gel filtration Streptomyces sp. G25
recombinant His-tagged enzyme from Escherichia coli strain C41(DE3) by ultracentrifugation and nickel affinity chromatography Streptomyces sp. G25

Source Tissue

Source Tissue Comment Organism Textmining
additional information Streptomyces strain G25 can accumulate extraordinarily large amounts of triacylglycerol (TAG) and consists of 64% fatty acids (dry weight) when cultivated with glucose and 50% fatty acids (dry weight) when cultivated with cellobiose Streptomyces sp. G25
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + a long-chain alcohol
-
 Streptomyces sp. G25 CoA + a long-chain ester
-
 ?
lauroyl-CoA + hexadecanol high activity Streptomyces sp. G25 hexadecanyl laurate + CoA
-
 ?
lauryl-CoA + 1,2-dipalmitoyl-sn-glycerol
-
 Streptomyces sp. G25 ?
-
 ?
additional information the purified enzyme AtfG25 shows acyltransferase activity with C12- or C16-acyl-CoA (cf. EC 2.3.1.20), C2 to C18 alcohols (ethanol, butanol, hexanol, octanol, decanol, dodecanol, tetradecanol, hexadecanol, palmitoleyl alcohol, and octadecanol), and dipalmitoyl glycerol. Substrate specificity, overview. The DGAT activity of AtfG25 corresponds to about 70% of itsWS activity Streptomyces sp. G25 ?
-
-
palmitoyl-CoA + 1,2-dipalmitoyl-sn-glycerol high activity Streptomyces sp. G25 ?
-
 ?
palmitoyl-CoA + 1-hexadecanol highest activity Streptomyces sp. G25 palmitic acid hexadecyl ester + CoA
-
 ?
palmitoyl-CoA + dodecanol
-
 Streptomyces sp. G25 dodecyl palmitate + CoA
-
 ?
palmitoyl-CoA + dodecanol
-
 Streptomyces sp. G25 CoA + dodecyl palmitate
-
 ?
palmitoyl-CoA + hexadecanol
-
 Streptomyces sp. G25 CoA + hexadecyl palmitate
-
 ?
palmitoyl-CoA + octadecanol lowest activity Streptomyces sp. G25 octadecyl palmitate + CoA
-
 ?
palmitoyl-CoA + octadecanol
-
 Streptomyces sp. G25 CoA + octadecyl palmitate
-
 ?
palmitoyl-CoA + palmitoleyl alcohol
-
 Streptomyces sp. G25 ?
-
 ?
palmitoyl-CoA + tetradecanol high activity Streptomyces sp. G25 tetradecyl palmitate + CoA
-
 ?
palmitoyl-CoA + tetradecanol
-
 Streptomyces sp. G25 CoA + tetradecyl palmitate
-
 ?

Subunits

Subunits Comment Organism
? x * 48740, calculated from amino acid sequence Streptomyces sp. G25

Synonyms

Synonyms Comment Organism
ATF
-
 Streptomyces sp. G25
AtfG25
-
 Streptomyces sp. G25
More see also EC 2.3.1.20 Streptomyces sp. G25
wax ester synthase/acyl CoA:diacylglycerol acyltransferase
-
 Streptomyces sp. G25
wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase
-
 Streptomyces sp. G25
WS/DGAT
-
 Streptomyces sp. G25

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Streptomyces sp. G25

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Streptomyces sp. G25

pI Value

Organism Comment pI Value Maximum pI Value
Streptomyces sp. G25 calculated from amino acid sequence
-
 9.53

General Information

General Information Comment Organism
evolution distribution of putative WS/DGAT in Streptomyces genomes, overview Streptomyces sp. G25
malfunction the respective gene, atfG25, is inactivated in Streptomyces sp. strain G25, cells of the insertion mutant still exhibit DGAT activity and are able to store TAG, albeit in lower quantities and at lower rates than the wild-type strain Streptomyces sp. G25
physiological function enzyme AtfG25 has an important, but not exclusive, role in triacylglycerol (TAG) biosynthesis in the Streptomyces sp. G25 isolate, suggesting the presence of alternative metabolic pathways for lipid accumulation Streptomyces sp. G25