Cloned (Comment) | Organism |
---|---|
gene DGAT1A, functional recombinant expression in Saccharomyces cerevisiae mutant strain H1246 and in Saccharomyces cerevisiae wild-type strain YPH499a, isozyme GmDGAT1A complements the TAG deficiency of mutant strain H1246. Recombinant expression in Arabidopsis thaliana using the Agrobacterium tumefaciens GV3101 transfection method. Quantitative RT-PCR enzyme expression analysis. Determination of the subcellular localization of GmDGAT-GFP performed by using tobacco leaf infiltration | Glycine max |
gene DGAT2D, functional recombinant expression in Saccharomyces cerevisiae mutant strain H1246 and in Saccharomyces cerevisiae wild-type strain YPH499a, isozyme GmDGAT2D complements the TAG deficiency of mutant strain H1246. Recombinant ectopic GmDGAT2D expression in soybean hairy roots, recombinant expression in Arabidopsis thaliana using the Agrobacterium tumefaciens GV3101 transfection method. Quantitative RT-PCR enzyme expression analysis. Determination of the subcellular localization of GmDGAT-GFP performed by using tobacco leaf infiltration | Glycine max |
Protein Variants | Comment | Organism |
---|---|---|
additional information | TAG biosynthesis and metabolic engineering of soybean oil with appropriate DGATs. GmDGAT1A-transgenic hairy roots synthesize more 18:3-triacylglycerols. Overexpression of GmDGAT1A in Arabidopsis thaliana seeds enhances the TAG production, GmDGAT1A enhances 18:3-TAG and reduces 20:1-TAG contents in rod1 mutant seeds | Glycine max |
additional information | TAG biosynthesis and metabolic engineering of soybean oil with appropriate DGATs. GmDGAT2D-transgenic hairy roots synthesize more linoleoyl- or oleoyl-triacylglycerols. Overexpression of GmDGAT2D in Arabidopsis thaliana seeds enhances the TAG production, GmDGAT2D promotes linoleoyl-TAG in wild-type but enhances oleoyl-TAG production in rod1 mutant seeds | Glycine max |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Glycine max | 5783 | - |
additional information | determination of the subcellular localization of GmDGAT-GFP performed by using tobacco leaf infiltration. Comparison of tissue expression patterns of isozymes GmDGAT1A and GmDGAT2D in nodules, roots, stems, leaves, flowers, pods, and seeds | Glycine max | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + 1,2-diacyl-sn-glycerol | Glycine max | - |
CoA + 1,2,3-triacylglycerol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | K7K424 | - |
- |
Glycine max | Q5GKZ7 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Glycine max | - |
flower | GmDGAT2D is mainly expressed in flower tissues | Glycine max | - |
leaf | - |
Glycine max | - |
additional information | GmDGAT1A is mainly expressed in seeds, but also in tissues like roots, leaves, flowers, at relatively lower levels | Glycine max | - |
additional information | GmDGAT2D transcripts are high in flowers and seeds, but also present in other tissues at lower levels, such as roots, leaves and pods | Glycine max | - |
pod | - |
Glycine max | - |
root | - |
Glycine max | - |
root nodule | - |
Glycine max | - |
seed | GmDGAT1A is highly expressed in seeds, the expression pattern is consistent with oil accumulation | Glycine max | - |
seed | high expression level, the expression pattern is consistent with oil accumulation | Glycine max | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + 1,2-diacyl-sn-glycerol | - |
Glycine max | CoA + 1,2,3-triacylglycerol | - |
? | |
linolenoyl-CoA + 1,2-diacyl-sn-glycerol | - |
Glycine max | CoA + 1,2-diacyl-3-linolenoyl-glycerol | - |
? | |
linoleoyl-CoA + 1,2-diacyl-sn-glycerol | - |
Glycine max | CoA + 1,2-diacyl-3-linoleoyl-glycerol | - |
? | |
additional information | substrate specificity of isozyme GmDGAT1A, comparison with isozyme GmDGAT2D, overview. GmDGAT1A prefers to use 18:3-acyl CoA for TAG synthesis | Glycine max | ? | - |
- |
|
additional information | substrate specificity of isozyme GmDGAT2D, comparison with isozyme GmDGAT1A, overview. GmDGAT2D prefers to use oleoyl-acyl CoA and linoleoyl-acyl CoA for TAG synthesis | Glycine max | ? | - |
- |
|
oleoyl-CoA + 1,2-diacyl-sn-glycerol | - |
Glycine max | CoA + 1,2-diacyl-3-oleoyl-glycerol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
diacylglycerol acyltransferase | - |
Glycine max |
diacylglycerol O-acyltransferase 1A | - |
Glycine max |
diacylglycerol O-acyltransferase 2D | - |
Glycine max |
GmDGAT1A | - |
Glycine max |
GmDGAT2D | - |
Glycine max |
Organism | Comment | Expression |
---|---|---|
Glycine max | GmDGAT2D is repressed by insect biting and jasmonate | down |
Glycine max | isozymes GmDGAT1A and 2D are differentially regulated by jasmonate during insect and wounding responses and abscisic acid for cold and heat stress response, indicating their different functions in soybean stress responses | additional information |
Glycine max | GmDGAT2D is upregulated by cold and heat stress and abscisic acid signaling | up |
General Information | Comment | Organism |
---|---|---|
additional information | quantification of triacylglycerol and analysis of fatty acid composition | Glycine max |
physiological function | isozyme GmDGAT1A may play a role in usual seed triacylglycerol (TAG) production. Isozymes GmDGAT1A and 2D are differentially regulated by jasmonate during insect and wounding responses and abscisic acid for cold and heat stress response, indicating their different functions in soybean stress responses | Glycine max |
physiological function | isozyme GmDGAT2D plays a role in usual seed triacylglycerol (TAG) production and is also involved in other tissues in responses to environmental and hormonal cues. Isozymes GmDGAT1A and 2D are differentially regulated by jasmonate during insect and wounding responses and abscisic acid for cold and heat stress response, indicating their different functions in soybean stress responses. Ectopic expression of GmDGATs in soybean hairy roots promotes TAG accumulation | Glycine max |