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Enzyme Nomenclature
Information on EC 2.3.1.20 - diacylglycerol O-acyltransferase
for references in articles please use BRENDA:EC2.3.1.20
Word Map on EC 2.3.1.20
- 2.3.1.20
- triacylglycerols
- triglyceride
- adipose
- acyl-coas
- cholesterol
- obesity
-
droplet
- cattle
- adipocytes
-
lipase
-
lipogenesis
-
proliferator-activated
-
dairy
-
lipogenic
- steatosis
- phosphatidate
-
high-fat
-
oleaginous
-
mgats
- glycerol-3-phosphate
-
lipolysis
- glycerolipids
-
holstein
-
esterification
-
pparg
-
lipotoxicity
- stearoyl-coa
-
oilseed
-
acyl-coa-dependent
-
biodiesel
- oleoyl-coa
- chylomicron
-
srebp-1c
-
kennedy
- acaca
-
srebf1
-
ricinoleic
-
acyl-coa:cholesterol
-
triacylglyceride
-
oleosins
-
3hglycerol
-
protein-1c
- mortierella
- sn-1,2-diacylglycerols
-
holstein-friesian
-
steryl
- glycerolphosphate
- sn-glycerol-3-phosphate
-
cholinephosphotransferase
- 2-monoacylglycerols
- medicine
- drug development
- biotechnology
- analysis
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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
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EC NUMBER 
COMMENTARY 
2.3.1.20
-
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RECOMMENDED NAME
GeneOntology No.
diacylglycerol O-acyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
acyl-CoA + 1,2-diacyl-sn-glycerol = CoA + triacylglycerol
-
-
-
-
acyl-CoA + 1,2-diacyl-sn-glycerol = CoA + triacylglycerol
the conserved sequence 161HPHG164 is important for activity
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Acyl group transfer
-
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
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SYSTEMATIC NAME
IUBMB Comments
acyl-CoA:1,2-diacyl-sn-glycerol O-acyltransferase
Palmitoyl-CoA and other long-chain acyl-CoAs can act as donors.
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CAS REGISTRY NUMBER
COMMENTARY
9029-98-5
-
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
bifunctional wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase, EC 2.3.1.75 and EC2.3.1.20 resp
Swissprot
-
486246, 486504, 486506, 659670, 672340, 674424, 676737, 704119, 704440, 706006, 719065, 720311, 735740, 735763, 735764, 736458, 737049
-
-
induction of several enzymic isoform genes upon entry of cells into nonreplicative drug-resistant state
-
-
-
486338, 486481, 486484, 486486, 486487, 486491, 486492, 486501, 660330, 675754, 701960, 704119, 704440, 705084, 719625, 720357
-
-
DGAT1, genomic sequence; tung tree, DGAT1 and DGAT2, both enzymes are encoded by single genes; DGAT1, genomic sequence; tung tree, DGAT1, single gene
SwissProt
member of the type 1 acyl-CoA:diacylglycerol acyltransferase family
UniProt
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
metabolism
physiological function
malfunction
-
Isoform DGAT1 inactivation promotes large lipid droplet formation. Inhibition of isoform DGAT2 augments fatty acid oxidation, whereas inhibition of DGAT1 increases triacylglycerol secretion. Triacylglycerol secretion is significantly reduced on DGAT2 inhibition without altering extracellular apolipoprotein B levels
malfunction
-
enzyme-deficient mice show no effect on retinal anatomy or the ultrastructure of photoreceptor outer-segments. Enzyme loss affects retinyl-ester synthesis and total acyl-coenzyme A:retinol acyl-transferase activityin the eye
metabolism
-
the triacylglycerol pool generated by DGAT1 is preferentially used for supplying substrates for oxidation, whereas DGAt2-derived triacylglycerol is more favored for secretion
physiological function
overexpression of the enzyme gene in Saccharomyces cerevisiae leads to accumulation of full-length prtein in wild-type and accumulation of full-length protein and a N-terminally truncated protein in a snf2 disruption mutant, lacking a DNA-dependent ATPase that forms the SWI/SNF chromatin remodeling complex. Proteolytic cleavage at the N-terminal region is involved in enzyme activation in the snf2 disruptant, a major cleavage site lies between residues Lys29 and Ser30
physiological function
enzyme makes a major contribution to triacylglycerol synthesis via an acyl-CoA-dependent mechanism and is not involved in steryl ester synthesis
physiological function
inhibition of isoform DGAT1 affects equally the incorporation of glycerol and exogenous preformed oleate into cellular and secreted triacylglycerol. Data indicate that isoform DGAT2 acts upstream of isoform DGAT1, and DGAT1 functions in the re-esterification of partial glycerides generated by intracellular lipolysis, using preformed fatty acids; isoform DGAT2 activity accounts for a modest fraction of less than 20% of overall cellular DGAT activity. Inhibition of DGAT2 activity specifically inhibits and is rate-limiting for the incorporation of de novo synthesized fatty acids and of glycerol into cellular and secreted triglyceride to a much greater extent than it affects the incorporation of exogenously added oleate. Isoform DGAT2 acts upstream of isoform DGAT1, largely determines the rate of de novo synthesis of triglyceride, and uses nascent diacylglycerol and de novo synthesized fatty acids as substrates
physiological function
upon overexpression in Mycobacterium smegmatis mc2155, cell morphology is changed and the cells become grossly enlarged. A massive formation of lipid bodies and a change in lipid pattern is observed simultaneously
physiological function
in isoforms dgat1, dgat2, dgat1/dgat2 double mutant and dgat1/dgat2/phospholipid:diacylglycerol acyltransferase triple mutant the total lipid% dry cell weight as a percentage of the wild-type strain is 57%, 36%, 18% and 13%, respectively; in isoforms dgat1, dgat2, dgat1/dgat2 double mutant and dgat1/dgat2/phospholipid:diacylglycerol acyltransferase triple mutant the total lipid% dry cell weight as a percentage of the wild-type strain is 57%, 36%, 18% and 13%, respectively
physiological function
-
the enzyme interacts with lipid droplets presumably to catalyze localized triacylglycerol synthesis for lipid droplet expansion
physiological function
-
upon overexpression in Mycobacterium smegmatis mc2155, cell morphology is changed and the cells become grossly enlarged. A massive formation of lipid bodies and a change in lipid pattern is observed simultaneously
-
physiological function
-
in isoforms dgat1, dgat2, dgat1/dgat2 double mutant and dgat1/dgat2/phospholipid:diacylglycerol acyltransferase triple mutant the total lipid% dry cell weight as a percentage of the wild-type strain is 57%, 36%, 18% and 13%, respectively; in isoforms dgat1, dgat2, dgat1/dgat2 double mutant and dgat1/dgat2/phospholipid:diacylglycerol acyltransferase triple mutant the total lipid% dry cell weight as a percentage of the wild-type strain is 57%, 36%, 18% and 13%, respectively
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
1,2-diacyl-sn-glycerol + erucoyl-CoA
1,2-diacyl-3-erucoyl-sn-glycerol + CoA
-
-
-
-
?
1,2-diacyl-sn-glycerol + oleoyl-CoA
1,2-diacyl-3-oleoyl-sn-glycerol + CoA
-
-
-
-
?
1,2-diacylglycerol + palmitoyl-CoA
1,2-diacyl-3-palmitoylglycerol + CoA
-
-
-
-
?
1,2-diacylglycerol + stearoyl-CoA
1,2-diacyl-3-stearoylglycerol + CoA
-
-
-
-
?
1,2-dilinolein + alpha-linolenoyl-CoA
1,2-dilinoleoyl-3-alpha-linolenoyl-sn-glycerol + CoA
-
-
-
?
1,2-dilinolein + linoleoyl-CoA
1,2,3-trilinoleoyl-sn-glycerol + CoA
-
-
-
?
1,2-dilinolein + oleoyl-CoA
1,2-dilinoleoyl-3-oleoyl-sn-glycerol + CoA
-
-
-
?
1,2-dilinolein + palmitoyl-CoA
1,2-dilinoleoyl-3-palmitoyl-sn-glycerol + CoA
-
-
-
?
1,2-dilinolenin + alpha-linolenoyl-CoA
1,2-dilinolenoyl-3-alpha-linolenoyl-sn-glycerol + CoA
-
-
-
?
1,2-dilinolenin + linoleoyl-CoA
1,2-dilinolenoyl-3-linoleoyl-sn-glycerol + CoA
-
-
-
?
1,2-dilinolenin + oleoyl-CoA
1,2-dilinolenoyl-3-oleoyl-sn-glycerol + CoA
-
-
-
?
1,2-dilinolenin + palmitoyl-CoA
1,2-dilinolenoyl-3-palmitoyl-sn-glycerol + CoA
-
-
-
?
1,2-diolein + alpha-linolenoyl-CoA
1,2-dioleoyl-3-alpha-linolenoyl-sn-glycerol + CoA
-
-
-
?
1,2-diolein + linoleoyl-CoA
1,2-dioleoyl-3-linoleoyl-sn-glycerol + CoA
-
-
-
?
1,2-diolein + oleoyl-CoA
1,2,3-trioleoyl-sn-glycerol + CoA
-
-
-
?
1,2-diolein + palmitoyl-CoA
1,2-dioleoyl-3-palmitoyl-sn-glycerol + CoA
-
-
-
?
1,2-dioleoyl-sn-glycerol + oleoyl-CoA
1,2,3-trioleoyl-sn-glycerol + CoA
-
-
-
-
?
1,2-dioleoyl-sn-glycerol + palmitoyl-CoA
1,2-dioleoyl-3-palmitoyl-sn-glycerol + CoA
-
-
-
-
?
1,2-dioleoylglycerol + arachidonoyl-CoA
1,2-dioleoyl-3-arachidonoylglycerol + CoA
-
about 8% of the activity with palmitoyl-CoA
-
-
?
1,2-dioleoylglycerol + linoleoyl-CoA
1,2-dioleoyl-3-linoleoylglycerol + CoA
-
about 12% of the activity with palmitoyl-CoA
-
-
?
1,2-dioleoylglycerol + stearoyl-CoA
1,2-dioleoyl-3-stearoylglycerol + CoA
-
about 10% of the activity with palmitoyl-CoA
-
-
?
1,2-dipalmitoyl-rac-glycerol + palmitoyl-CoA
tripalmitoylglycerol + CoA
-
-
-
?
1,2-dipalmitoyl-sn-glycerol + oleoyl-CoA
1,2-dipalmitoyl-3-oleoyl-sn-glycerol + CoA
-
-
-
?
1,2-dipalmitoyl-sn-glycerol + palmitoyl-CoA
tripalmitin + CoA
-
-
-
?
1,2-dipalmitoyl-sn-glycerol + stearoyl-CoA
1,2-dipalmitoyl-3-stearoyl-sn-glycerol + CoA
-
-
-
?
1,2-diricinoleoyl-sn-glycerol + ricinoleoyl-CoA
triricinolein + CoA
-
-
-
-
?
1-palmitoyl-2-oleoylglycerol + acyl-CoA
1-palmitoyl-2-oleoyl-3-acylglycerol + CoA
-
-
-
-
?
acyl-CoA + 1,2-didecanoylglycerol
CoA + 3-acyl-1,2-didecanoylglycerol
-
-
-
-
?
acyl-CoA + 1,2-dihexanoylglycerol
CoA + 3-acyl-1,2-dihexanoylglycerol
-
-
-
-
?
acyl-CoA + 1,2-dilauroylglycerol
CoA + 3-acyl-1,2-dilauroylglycerol
-
-
-
-
?
acyl-CoA + 1,2-dimyristoylglycerol
CoA + 3-acyl-1,2-dimyristoylglycerol
-
-
-
-
?
acyl-CoA + 1,2-dioctanoylglycerol
CoA + 3-acyl-1,2-dioctanoylglycerol
-
-
-
-
?
acyl-CoA + 1,2-dipalmitin
CoA + 3-acyl-1,2-dipalmitoylglycerol
-
no acceptor
-
-
?
acyl-CoA + 1,2-dipalmitoylglycerol
CoA + 3-acyl-1,2-dipalmitoylglycerol
-
membrane bound 1,2-dipalmitoylglycerol
-
-
?
acyl-CoA + 1-decanol
? + CoA
at 48% of the activity with hexanol
-
-
?
acyl-CoA + 2,3-dioleoyl-sn-glycerol
CoA + 1-acyl-2,3-dioleoyl-sn-glycerol
-
-
-
-
?
acyl-CoA + 2-cyclohexylethanol
? + CoA
at 45% of the activity with hexanol
-
-
?
acyl-CoA + 2-decanol
? + CoA
at 39% of the activity with hexanol
-
-
?
acyl-CoA + 4-decanol
? + CoA
at 15.6% of the activity with hexanol
-
-
?
acyl-CoA + cyclododecanol
? + CoA
at 80% of the activity with hexanol
-
-
?
acyl-CoA + cyclohexandiol
? + CoA
at 4.1% of the activity with hexanol
-
-
?
acyl-CoA + cyclohexanol
? + CoA
at 32% of the activity with hexanol
-
-
?
acyl-CoA + cyclohexanone oxime
? + CoA
at 5.2% of the activity with hexanol
-
-
?
acyl-CoA + lysophosphatidylcholine
CoA + acyllysophosphatidylcholine
-
-
-
-
?
acyl-CoA + phenol
? + CoA
at 4.1% of the activity with hexanol
-
-
?
acyl-CoA + phenylethanol
? + CoA
at 99% of the activity with hexanol
-
-
?
acyl-CoA + rac-1,2-diacetylglycerol
CoA + 3-acyl-rac-1,2-diacetylglycerol
-
no acceptor
-
-
?
acyl-CoA + rac-1,2-dibutyrylglycerol
CoA + 3-acyl-rac-1,2-dibutyrylglycerol
-
no acceptor
-
-
?
alpha-linolenoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + 1,2-dioleoyl-3-linolenoyl-sn-glycerol
100% activity
-
-
?
caproyl-CoA + 1,2-dicaproyl-sn-glycerol
CoA + tricaproylglycerol
-
-
-
-
?
decanoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-decanoylglycerol
-
saturated fatty acyl-CoAs from C-8 to C-18 with decanoyl-CoA as the best
-
-
?
erucoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + 3-erucoyl-1,2-dioleoyl-sn-glycerol
-
-
-
-
?
linoleoyl-CoA + 1,2-dioleoyl-sn-glycerol
1,2-dioleoyl-3-linoleoyl-sn-glycerol + CoA
-
-
-
-
?
linoleoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + 1,2-dioleoyl-3-linoleoyl-sn-glycerol
-
-
-
?
linoleoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + 3-linoleoyl-1,2-dioleoylglycerol
-
i.e. diolein
-
?
N-[(7-nitro-2-1,3-benzoxadiazol-4-yl)]aminopalmitoyl-CoA + 1,2-dipalmitoyl-sn-glycerol
CoA + 3-[N-[(7-nitro-2-1,3-benzoxadiazol-4-yl)]-16-aminopalmitoyl]-1,2-dioleoyl-sn-glycerol
-
-
-
-
?
octanoyl-CoA + 1,2-di-(cis-9-octadecenoyl)-sn-glycerol
CoA + 1,2-di-(cis-9-octadecenoyl)-3-octanoyl-sn-glycerol
-
-
-
-
?
oleoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + 1,2,3-trioleoyl-sn-glycerol
about 85% activity compared to alpha-linolenoyl-CoA
-
-
?
oleoyl-CoA + 1,2-dioleoylglycerol
CoA + 1,2,3-trioleoylglycerol
-
-
-
?
oleoyl-CoA + 1,2-dipalmitoylglycerol
CoA + 3-oleoyl-1,2-dipalmitoylglycerol
-
-
-
?
oleoyl-CoA + 1,2-diricinoloylglycerol
CoA + 3-oleoyl-1,2-diricinoloylglycerol
best substrate
-
-
?
oleoyl-CoA + 1,2-divernoloyl-sn-glycerol
CoA + 1,2-divernoloyl-3-oleoyl-sn-glycerol
oleoyl-CoA and 1,2-dioleoyl-sn-glycerol are preferred substrates over vernoloyl-CoA and 1,2-divernoloyl-sn-glycerol
-
-
?
palmitoleoyl-CoA + 1,2-dipalmitoleoyl-sn-glycerol
CoA + tripalmitoleoylglycerol
-
preferred substrate for isoform DGAT2
-
-
?
palmitoyl-CoA + 1,2-dipalmitoylglycerol
CoA + tripalmitin
approximately 10fold lower level of diacylglycerol acyltransferase activity
-
-
?
palmitoyl-CoA + 1,2-distearin
CoA + 3-palmitoyl-1,2-distearoylglycerol
-
-
-
-
?
palmitoyl-CoA + 1,3-diolein
CoA + 1,3-dioleoyl-2-palmitoylglycerol
-
-
-
-
?
palmitoyl-CoA + didecanoylglycerol
CoA + 1,2-didecanoyl-3-palmitoylglycerol
-
i.e. dicaprin
-
-
?
palmitoyl-CoA + dihexanoylglycerol
CoA + dihexanoyl-palmitoylglycerol
-
i.e. dicaproin
-
-
?
palmitoyl-CoA + dipalmitoylglycerol
CoA + tripalmitoylglycerol
-
membrane-bound dipalmitoylglycerol
-
-
?
palmitoyl-CoA + sn-2-monooleoylglycerol
CoA + 1-palmitoyl-2-oleoylglycerol
-
-
-
-
-
ricinoleoyl-CoA + 1,2-dioleoyl-sn-glycerol
1,2-dioleoyl-3-ricinoleoyl-sn-glycerol + CoA
-
-
-
-
?
ricinoleoyl-CoA + 1,2-dipalmitoyl-sn-glycerol
1,2-dipalmitoyl-3-ricinoleoyl-sn-glycerol + CoA
-
prefers acyl acceptor 1,2-dioleoyl-sn-glycerol over 1,2-dipalmitoyl-sn-glycerol
-
-
?
sn-1,2-diacylglycerol + erucoyl-CoA
triacylglycerol + CoA
-
i.e. a 22:1cisDELTA13-CoA
-
-
?
stearoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + 1,2-dioleoyl-3-stearoyl-sn-glycerol
-
-
-
?
vernoloyl-CoA + 1,2-divernoloyl-sn-glycerol
CoA + trivernoloyl-sn-glycerol
oleoyl-CoA and 1,2-dioleoyl-sn-glycerol are preferred substrates over vernoloyl-CoA and 1,2-divernoloyl-sn-glycerol
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
role in leaf metabolism, overview
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
the enzyme catalyzes the final step in triacylglycerol biosynthesis that acylates diacylglycerol to triacylglycerols
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
the enzyme catalyzes the final step in triacylglycerol biosynthesis that acylates diacylglycerol to triacylglycerols
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
DGAT catalyzes the transfer of an acyl moiety between two DAG molecules to form triacylglycerol and monoacylglycerol
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
Cuphea sp.
-
microsomal DGAT from Cuphea exhibits high activity toward diacylglycerols containing unusual fatty acids, e.g. lauric acids, at both sn-1 and sn-2 positions, acyl-CoA specificity, overview
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
-
acyl-CoA specificity, overview
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
-
acyl-CoA specificity, overview
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
-
acyl-CoA specificity, overview
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
microsomal DGAT from castor bean exhibited high activity toward diacylglycerols containing unusual fatty acids, e.g. ricinoleic acids, at both sn-1 and sn-2 positions, acyl-CoA specificity, overview
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
-
acyl-CoA specificity, overview
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
-
acyl-CoA specificity, no acylation activity with phosphoatidylcholine or oleic acid, overview
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
DGAT1 and DGAT2 are two unrelated enzymes that catalyze the committed step in triacylglycerol biosynthesis
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
DGAT1 catalyzes the committed step in triacylglycerol biosynthesis
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
DGAT2 catalyzes the committed step in triacylglycerol biosynthesis
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
-
acyl-CoA specificity, overview
-
-
?
1,2-dioleoylglycerol + oleoyl-CoA
triolein + CoA
-
about 90% of the activity with palmitoyl-CoA
-
-
?
1,2-dioleoylglycerol + palmitoyl-CoA
1,2-dioleoyl-3-palmitoyl-glycerol + CoA
-
-
-
-
?
1,2-dioleoylglycerol + palmitoyl-CoA
1,2-dioleoyl-3-palmitoyl-glycerol + CoA
-
-
-
-
?
1,2-dioleoylglycerol + palmitoyl-CoA
1,2-dioleoyl-3-palmitoylglycerol + CoA
-
-
-
-
?
1,2-dioleoylglycerol + palmitoyl-CoA
1,2-dioleoyl-3-palmitoylglycerol + CoA
-
-
-
?
1,2-dipalmitoyl-rac-glycerol + palmitoyl-CoA
rac-tripalmitoylglycerol + CoA
-
-
-
?
1,2-dipalmitoyl-rac-glycerol + palmitoyl-CoA
rac-tripalmitoylglycerol + CoA
-
-
-
?
1-palmitoyl-2-oleoyl glycerol + acyl-CoA
1-palmitoyl-2-oleoyl-3-acylglycerol + CoA
-
-
-
-
?
1-palmitoyl-2-oleoyl glycerol + acyl-CoA
1-palmitoyl-2-oleoyl-3-acylglycerol + CoA
-
-
-
-
?
1-palmitoyl-2-oleoyl glycerol + acyl-CoA
1-palmitoyl-2-oleoyl-3-acylglycerol + CoA
Cuphea sp.
-
-
-
-
?
1-palmitoyl-2-oleoyl glycerol + acyl-CoA
1-palmitoyl-2-oleoyl-3-acylglycerol + CoA
-
-
-
-
?
1-palmitoyl-2-oleoyl glycerol + acyl-CoA
1-palmitoyl-2-oleoyl-3-acylglycerol + CoA
-
-
-
-
?
1-palmitoyl-2-oleoyl glycerol + acyl-CoA
1-palmitoyl-2-oleoyl-3-acylglycerol + CoA
-
-
-
-
?
1-palmitoyl-2-oleoyl glycerol + acyl-CoA
1-palmitoyl-2-oleoyl-3-acylglycerol + CoA
-
-
-
-
?
1-palmitoyl-2-oleoyl glycerol + acyl-CoA
1-palmitoyl-2-oleoyl-3-acylglycerol + CoA
-
-
-
-
?
1-palmitoyl-2-oleoyl glycerol + acyl-CoA
1-palmitoyl-2-oleoyl-3-acylglycerol + CoA
-
-
-
-
?
1-palmitoyl-2-oleoyl glycerol + acyl-CoA
1-palmitoyl-2-oleoyl-3-acylglycerol + CoA
-
-
-
-
?
1-palmitoyl-2-oleoyl glycerol + acyl-CoA
1-palmitoyl-2-oleoyl-3-acylglycerol + CoA
-
-
-
-
?
acyl-CoA + 1,2-diacyl-sn-glycerol
CoA + triacylglycerol
-
-
-
?
acyl-CoA + 1,2-diacyl-sn-glycerol
CoA + triacylglycerol
isoform DGAT2b is catalytically more active than DGAt2a
-
-
?
acyl-CoA + 1,2-diacyl-sn-glycerol
CoA + triacylglycerol
isoform DGAT2b is catalytically more active than DGAt2a
-
-
?
acyl-CoA + 1,2-diacyl-sn-glycerol
CoA + triacylglycerol
-
-
-
?
acyl-CoA + 1,2-diacyl-sn-glycerol
CoA + triacylglycerol
-
-
-
?
acyl-CoA + 1,2-diacyl-sn-glycerol
CoA + triacylglycerol
-
-
-
?
acyl-CoA + 1,2-diacyl-sn-glycerol
CoA + triacylglycerol
-
isoforms DGAT1 and DGAT2 Can compensate for each other to synthesize triacylglycerol
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
-
saturated fatty acyl-CoAs from C-4 to C-18
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
-
broad range of donors from C-12 to C-22, broad range of acceptors from C12 to C-22
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
-
-
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
-
enzyme catalyzing the final reaction in the sn-glycerol-3-phosphate pathway leading to TAG
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
-
acceptors: 1,2-diacylglycerol containing oleic acid and 1,2-diacylglycerol containing capric acid
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
-
last step of triacylglycerol synthesis
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
acceptors: overview
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
-
DGAT1 is more involved in fat absorption in the intestine and DGAT2 plays important role in assembly of de novo synthesized fatty acids into VLDL particles in liver
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
-
endogenously and exogenously synthesized diacylglycerol
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
-
acyl-donors: broad specificity, saturated, mono-, di- and tetraenoic thioesters
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
-
saturated fatty acyl-CoAs from C-8 to C-12
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
-
2,3-diacylglycerol is acylated at 20% the rate of 1,2-isomer
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
-
last reaction in triacylglycerol synthesis
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
-
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
-
endogenous 1,2-diacylglycerol
-
-
?
acyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + trioleoylglycerol
-
-
-
-
?
acyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + trioleoylglycerol
-
-
-
-
?
acyl-CoA + 1,2-dioleoylglycerol
CoA + 3-acyl-1,2-dioleoylglycerol
-
-
-
-
?
acyl-CoA + 1,2-dioleoylglycerol
CoA + 3-acyl-1,2-dioleoylglycerol
-
-
-
-
?
acyl-CoA + 1,2-dioleoylglycerol
CoA + 3-acyl-1,2-dioleoylglycerol
-
-
-
-
?
acyl-CoA + 1,3-dioleoylglycerol
CoA + 2-acyl-1,3-dioleoylglycerol
-
no acceptor
-
-
?
acyl-CoA + 1,3-dioleoylglycerol
CoA + 2-acyl-1,3-dioleoylglycerol
-
-
-
-
?
acyl-CoA + rac-1,2-dioleoylglycerol
CoA + 3-acyl-rac-1,2-dioleoylglycerol
-
-
-
-
?
acyl-CoA + rac-1,2-dioleoylglycerol
CoA + 3-acyl-rac-1,2-dioleoylglycerol
-
-
-
-
?
arachidonoyl-CoA + 1,2-diacylglycerol
CoA + 3-arachidonoyl-1,2-diacylglycerol
-
-
-
?
arachidonoyl-CoA + 1,2-diacylglycerol
CoA + 3-arachidonoyl-1,2-diacylglycerol
-
-
-
-
?
butyryl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-butyrylglycerol
-
-
-
-
?
butyryl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-butyrylglycerol
-
-
-
-
?
butyryl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-butyrylglycerol
-
-
-
-
?
erucoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-erucoylglycerol
-
Jet Neuf enzyme displays an enhanced specificity for erucoyl-CoA over oleoyl-CoA
-
-
-
erucoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-erucoylglycerol
-
-
-
-
?
lauroyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-lauroylglycerol
-
-
-
-
?
lauroyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-lauroylglycerol
-
-
-
-
?
linoleoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-linoleoylglycerol
-
-
-
-
?
linoleoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-linoleoylglycerol
-
-
-
?
linoleoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-linoleoylglycerol
-
-
-
?
linoleoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-linoleoylglycerol
-
-
-
-
-
linoleoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-linoleoylglycerol
-
poor donor
-
-
?
myristoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-myristoylglycerol
-
-
-
-
?
myristoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-myristoylglycerol
-
-
-
-
?
oleoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-oleoylglycerol
-
-
-
-
?
oleoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-oleoylglycerol
-
-
-
-
?
oleoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-oleoylglycerol
-
-
-
-
?
oleoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-oleoylglycerol
-
-
-
-
?
oleoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-oleoylglycerol
-
-
-
-
?
oleoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-oleoylglycerol
-
-
-
?
oleoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-oleoylglycerol
-
-
-
?
oleoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-oleoylglycerol
-
-
-
-
?
oleoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-oleoylglycerol
-
-
-
-
?
oleoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + triolein
oleoyl-CoA and 1,2-dioleoyl-sn-glycerol are preferred substrates over vernoloyl-CoA and 1,2-divernoloyl-sn-glycerol
-
-
?
oleoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + trioleoylglycerol
-
-
-
-
?
oleoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + trioleoylglycerol
-
-
-
-
?
oleoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + trioleoylglycerol
-
-
-
?
oleoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + trioleoylglycerol
-
i.e. diolein, at 34% the rate of the acylation with stearoyl-CoA
-
-
-
oleoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + trioleoylglycerol
-
-
-
-
?
oleoyl-CoA + diolein
CoA + triolein
-
-
-
?
oleoyl-CoA + diolein
CoA + triolein
-
-
-
?
oleoyl-CoA + sn-2-monooleoylglycerol
CoA + 1,2-dioleoyl-sn-glycerol
-
-
-
-
?
palmitoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-palmitoylglycerol
-
-
-
-
?
palmitoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-palmitoylglycerol
-
-
-
-
?
palmitoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-palmitoylglycerol
-
-
-
-
?
palmitoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-palmitoylglycerol
-
-
-
-
?
palmitoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-palmitoylglycerol
-
-
-
-
?
palmitoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-palmitoylglycerol
-
-
-
-
?
palmitoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-palmitoylglycerol
-
diacylglycerol diluted in ethanol
-
-
?
palmitoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + 1,2-dioleoyl-3-palmitoyl-sn-glycerol
about 85% activity compared to alpha-linolenoyl-CoA
-
-
?
palmitoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + 1,2-dioleoyl-3-palmitoyl-sn-glycerol
-
-
-
-
?
palmitoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + 1,2-dioleoyl-3-palmitoyl-sn-glycerol
-
-
-
?
palmitoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + 1,2-dioleoyl-3-palmitoylglycerol
-
-
-
-
?
palmitoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + 1,2-dioleoyl-3-palmitoylglycerol
-
-
-
-
?
palmitoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + 1,2-dioleoyl-3-palmitoylglycerol
-
i.e. diolein
-
-
?
palmitoyl-CoA + 1,2-dipalmitoyl-sn-glycerol
CoA + tripalmitoylglycerol
-
preferred substrate for isoform DGAT1
-
-
?
palmitoyl-CoA + 1,2-dipalmitoyl-sn-glycerol
CoA + tripalmitoylglycerol
-
-
-
-
?
sn-1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
-
the final step in triacylglycerol synthesis is catalyzed by the acyl-CoA:diacylglycerol acyltransferase enzymes, DGAT1 and DGAT2
-
-
?
sn-1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
-
the enzyme contains a neutral lipid binding sequence 80FLVLGVAC87 residing in the first transmembrane domain
-
-
?
stearoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-stearoylglycerol
-
weak donor
-
-
?
stearoyl-CoA + 1,2-diacylglycerol
CoA + 1,2-diacyl-3-stearoylglycerol
-
weak donor
-
-
?
stearoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + 1,2-dioleoyl-3-stearoylglycerol
-
-
-
-
?
stearoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + 1,2-dioleoyl-3-stearoylglycerol
-
-
-
-
-
stearoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + 1,2-dioleoyl-3-stearoylglycerol
-
i.e. diolein
-
-
?
additional information
?
-
ATfA exhibits a clear preference for the acylation of the sn-2 position of sn-1,2-dipalmitoylglycerol rather than the sn-2 position of sn-1,3-dipalmitoylglycerol
-
-
-
additional information
?
-
-
the bifunctional wax ester synthase/acyl coenzyme A (acyl-CoA):diacylglycerol acyltransferase from Acinetobacter sp. strain ADP1 mediates the biosyntheses of wax esters and triacylglycerols
-
-
-
additional information
?
-
-
the bifunctional enzyme catalyzes the reactions of the diacylglycerol transferase, EC 2.3.1.20, and of the wax synthase, EC 2.3.1.75
-
-
-
additional information
?
-
enzyme also has acyl-CoA-monoacylglycerol acyltransferase activity, sn-1 and sn-3 positions are accepted with higher specificity than sn-2 position
-
-
-
additional information
?
-
rate of activity is highly dependent on acyl composition with highest activities for acyl groups containing several double bonds, epoxy, or hydroxy groups. Enzyme uses both sn-positions of phosphatidylcholine with 3fold preference for sn-2 position
-
-
-
additional information
?
-
-
substrate specificity of native and recombinant enzyme, the native and the recombinant enzyme do not show wax ester synthase activity in contrast to other DGATs, no activity with hexadecanol, glycerol-3-phosphate, monoacylglycerol, lysophosphatidic acid, and lysophosphatidylcholine, oleoyl-CoA is the preferred acyl donor as compared to palmitoyl- and stearoyl-CoAs
-
-
-
additional information
?
-
substrate specificity of native and recombinant enzyme, the native and the recombinant enzyme do not show wax ester synthase activity in contrast to other DGATs, no activity with hexadecanol, glycerol-3-phosphate, monoacylglycerol, lysophosphatidic acid, and lysophosphatidylcholine, oleoyl-CoA is the preferred acyl donor as compared to palmitoyl- and stearoyl-CoAs
-
-
-
additional information
?
-
substrate specificity of native and recombinant enzyme, the native and the recombinant enzyme do not show wax ester synthase activity in contrast to other DGATs, no activity with hexadecanol, glycerol-3-phosphate, monoacylglycerol, lysophosphatidic acid, and lysophosphatidylcholine, oleoyl-CoA is the preferred acyl donor as compared to palmitoyl- and stearoyl-CoAs
-
-
-
additional information
?
-
-
no activity with sn-1,3-diacylglycerols, water-soluble diacylglycerols, including rac-1,2-diacetylglycerol and rac-1,2-dibutyrylglycerol, are poor substrates of DGAT from safflower seeds, acyl-CoA specificity, overview
-
-
-
additional information
?
-
-
increased lipid synthesis has variable effects on diacylglycerol accumulation, overview, regulation of lipid biosynthesis in cultures tissues, changes in the endogenous activity of DAGAT is unlikely to affect oil accumulation in oil palm crops, overview
-
-
-
additional information
?
-
-
the multifunctional enzyme plays an important role in lipid metabolism in human skin
-
-
-
additional information
?
-
-
DGAT1 is catalyzing the reactions of the monoacylglycerol transferase, EC 2.3.1.22, of the diacylglycerol transferase, EC 2.3.1.20, of the wax synthase, EC 2.3.1.75, and of the acyl-CoA:retinol acyltransferase, EC 2.3.1.76, overview
-
-
-
additional information
?
-
-
the enzyme also performs the reaction of the retinol O-fatty-acyltransferase, EC 2.3.1.76
-
-
-
additional information
?
-
-
enzyme displays striking preference to palmitoyl-CoA and oleoyl-CoA as acyl donors and utilizes 1,2-diacylglycerol as acceptor
-
-
-
additional information
?
-
-
the enzyme interacts with monoacylglycerol acyltransferase-2
-
-
-
additional information
?
-
-
co-expressing flax diacylglycerol acyltransferase1-1 (DGAT1-1) and acyl-CoA:lysophosphatidylcholine acyltransferase1 (LPCAT1) in a yeast quintuple mutant significantly increases 18-carbon polyunsaturated fatty acids in triacylglycerol with a concomitant decrease of 18-carbon polyunsaturated fatty acids in phospholipid. The specific activity of overall LPCAT1 and DGAT1-1 coupling process exhibited a preference for transferring 14C-labeled linoleoyl or linolenoyl than oleoyl moieties from the sn-2 position of phosphatidylcholine to triacylglycerol
-
-
-
additional information
?
-
the enzyme shows a substrate preference for monounsaturated over polyunsaturated fatty acids
-
-
-
additional information
?
-
-
the enzyme shows a substrate preference for monounsaturated over polyunsaturated fatty acids
-
-
-
additional information
?
-
-
the enzyme shows very low activity with ethanol, methanol, butanol, and glycerol
-
-
-
additional information
?
-
increasing the cytoplasmic triglyceride pool in hepatocytes does not directly influence VLDL triglyceride or apoB production, overview
-
-
-
additional information
?
-
increasing the cytoplasmic triglyceride pool in hepatocytes does not directly influence VLDL triglyceride or apoB production, overview
-
-
-
additional information
?
-
-
DGAT is catalyzing the reactions of the monoacylglycerol transferase, EC 2.3.1.22, of the diacylglycerol transferase, EC 2.3.1.20, of the wax synthase, EC 2.3.1.75, and of the acyl-CoA:retinol acyltransferase, EC 2.3.1.76, overview
-
-
-
additional information
?
-
DGAT1 is also catalyzing the reactions of the monoacylglycerol transferase, EC 2.3.1.22, of the wax synthase, EC 2.3.1.75, and of the acyl-CoA:retinol acyltransferase, EC 2.3.1.76, overview
-
-
-
additional information
?
-
DGAT1 is also catalyzing the reactions of the monoacylglycerol transferase, EC 2.3.1.22, of the wax synthase, EC 2.3.1.75, and of the acyl-CoA:retinol acyltransferase, EC 2.3.1.76, overview
-
-
-
additional information
?
-
DGAT1 is catalyzing the reactions of the monoacylglycerol transferase, EC 2.3.1.22, of the diacylglycerol transferase, EC 2.3.1.20, of the wax synthase, EC 2.3.1.75, and of the acyl-CoA:retinol acyltransferase, EC 2.3.1.76, overview
-
-
-
additional information
?
-
DGAT1 is catalyzing the reactions of the monoacylglycerol transferase, EC 2.3.1.22, of the diacylglycerol transferase, EC 2.3.1.20, of the wax synthase, EC 2.3.1.75, and of the acyl-CoA:retinol acyltransferase, EC 2.3.1.76, overview
-
-
-
additional information
?
-
enzyme mediates the transesterification of diacylglycerol using long-chain acyl-CoA as acyl donors. In addition, it functions as mycolyltransferase, a docking model suggests that palmitoleoyl-coenzyme A and 1,2-dipalmitin occupy the same active site as trehalose 6,6-dimycolate and trehalose 6-monomycolate
-
-
-
additional information
?
-
enzyme mediates the transesterification of diacylglycerol using long-chain acyl-CoA as acyl donors. In addition, it functions as mycolyltransferase, a docking model suggests that palmitoleoyl-coenzyme A and 1,2-dipalmitin occupy the same active site as trehalose 6,6-dimycolate and trehalose 6-monomycolate
-
-
-
additional information
?
-
-
increased lipid synthesis has variable effects on diacylglycerol accumulation, overview, regulation of lipid biosynthesis in cultures tissues, changes in the endogenous activity of DAGAT is unlikely to affect oil accumulation in oil palm crops, overview
-
-
-
additional information
?
-
-
DGAT3 protein has two catalytic domains: a wax ester synthase-like acyl-CoA acyltransferase domain and a bacteria-specific acyltransferase domain and shows a significant preference to endogenous benzeneacetic acid and octadecenoic acid
-
-
-
additional information
?
-
-
no activity with sn-1,3-diacylglycerols, acyl-CoA specificity, overview
-
-
-
additional information
?
-
-
the enzyme prefers unsaturated fatty acids over saturated ones
-
-
-
additional information
?
-
-
the enzyme uses oleic, palmitic, stearic, and linoleic acid with different activities
-
-
-
additional information
?
-
-
the enzyme prefers unsaturated fatty acids over saturated ones
-
-
-
additional information
?
-
-
the enzyme uses oleic, palmitic, stearic, and linoleic acid with different activities
-
-
-
additional information
?
-
-
in castor bean DGAT2 is more likely to play a major role in seed triacylglycerol biosynthesis than DGAT1
-
-
-
additional information
?
-
-
mechanism for the degradation of the DGAT protein, overview
-
-
-
additional information
?
-
the enzyme uses oleic, palmitic, stearic, and linoleic acid with different activities
-
-
-
additional information
?
-
-
the enzyme uses oleic, palmitic, stearic, and linoleic acid with different activities
-
-
-
additional information
?
-
the enzyme uses oleic, palmitic, stearic, and linoleic acid with different activities
-
-
-
additional information
?
-
recombinant TmDGAT1 protein is capable of utilizing a range of (14)C-labelled fatty acyl-CoA donors and diacylglycerol acceptors, and can synthesize (14)C-trierucin
-
-
-
additional information
?
-
-
recombinant TmDGAT1 protein is capable of utilizing a range of (14)C-labelled fatty acyl-CoA donors and diacylglycerol acceptors, and can synthesize (14)C-trierucin
-
-
-
additional information
?
-
-
DGAT is also catalyzing the reactions of the monoacylglycerol transferase, EC 2.3.1.22
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
1,2-diricinoleoyl-sn-glycerol + ricinoleoyl-CoA
triricinolein + CoA
-
-
-
-
?
octanoyl-CoA + 1,2-di-(cis-9-octadecenoyl)-sn-glycerol
CoA + 1,2-di-(cis-9-octadecenoyl)-3-octanoyl-sn-glycerol
-
-
-
-
?
palmitoyl-CoA + sn-2-monooleoylglycerol
CoA + 1-palmitoyl-2-oleoylglycerol
-
-
-
-
-
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
Q9SLD2
role in leaf metabolism, overview
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
Q2KP14
the enzyme catalyzes the final step in triacylglycerol biosynthesis that acylates diacylglycerol to triacylglycerols
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
Q2KP14
the enzyme catalyzes the final step in triacylglycerol biosynthesis that acylates diacylglycerol to triacylglycerols
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
Q9XGR5, Q9XGV4
DGAT catalyzes the transfer of an acyl moiety between two DAG molecules to form triacylglycerol and monoacylglycerol
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
Q0QJH9, Q0QJI1
DGAT1 and DGAT2 are two unrelated enzymes that catalyze the committed step in triacylglycerol biosynthesis
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
Q0QJH9, Q0QJI1
DGAT1 catalyzes the committed step in triacylglycerol biosynthesis
-
-
?
1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
Q0QJH9, Q0QJI1
DGAT2 catalyzes the committed step in triacylglycerol biosynthesis
-
-
?
1,2-dioleoylglycerol + palmitoyl-CoA
1,2-dioleoyl-3-palmitoylglycerol + CoA
-
-
-
-
?
1,2-dioleoylglycerol + palmitoyl-CoA
1,2-dioleoyl-3-palmitoylglycerol + CoA
Q9DCV3, Q9Z2A7
-
-
-
?
acyl-CoA + 1,2-diacyl-sn-glycerol
CoA + triacylglycerol
G3GD57, G3GD58
-
-
-
?
acyl-CoA + 1,2-diacyl-sn-glycerol
CoA + triacylglycerol
A0A023PJY4, A0A023PKP7
isoform DGAT2b is catalytically more active than DGAt2a
-
-
?
acyl-CoA + 1,2-diacyl-sn-glycerol
CoA + triacylglycerol
A0A023PJY4, A0A023PKP7
isoform DGAT2b is catalytically more active than DGAt2a
-
-
?
acyl-CoA + 1,2-diacyl-sn-glycerol
CoA + triacylglycerol
A0A096XN58
-
-
-
?
acyl-CoA + 1,2-diacyl-sn-glycerol
CoA + triacylglycerol
A0A096XN58
-
-
-
?
acyl-CoA + 1,2-diacyl-sn-glycerol
CoA + triacylglycerol
A0A0U3E0G8
-
-
-
?
acyl-CoA + 1,2-diacyl-sn-glycerol
CoA + triacylglycerol
-
isoforms DGAT1 and DGAT2 Can compensate for each other to synthesize triacylglycerol
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
-
enzyme catalyzing the final reaction in the sn-glycerol-3-phosphate pathway leading to TAG
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
-
last step of triacylglycerol synthesis
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
-
DGAT1 is more involved in fat absorption in the intestine and DGAT2 plays important role in assembly of de novo synthesized fatty acids into VLDL particles in liver
-
-
?
acyl-CoA + 1,2-diacylglycerol
CoA + triacylglycerol
-
last reaction in triacylglycerol synthesis
-
-
?
acyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + trioleoylglycerol
-
-
-
-
?
acyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + trioleoylglycerol
-
-
-
-
?
oleoyl-CoA + 1,2-dioleoyl-sn-glycerol
CoA + trioleoylglycerol
R9YW17
-
-
-
?
sn-1,2-diacylglycerol + acyl-CoA
triacylglycerol + CoA
-
the final step in triacylglycerol synthesis is catalyzed by the acyl-CoA:diacylglycerol acyltransferase enzymes, DGAT1 and DGAT2
-
-
?
additional information
?
-
-
the bifunctional wax ester synthase/acyl coenzyme A (acyl-CoA):diacylglycerol acyltransferase from Acinetobacter sp. strain ADP1 mediates the biosyntheses of wax esters and triacylglycerols
-
-
-
additional information
?
-
-
increased lipid synthesis has variable effects on diacylglycerol accumulation, overview, regulation of lipid biosynthesis in cultures tissues, changes in the endogenous activity of DAGAT is unlikely to affect oil accumulation in oil palm crops, overview
-
-
-
additional information
?
-
-
the multifunctional enzyme plays an important role in lipid metabolism in human skin
-
-
-
additional information
?
-
Q9DCV3
increasing the cytoplasmic triglyceride pool in hepatocytes does not directly influence VLDL triglyceride or apoB production, overview
-
-
-
additional information
?
-
Q9Z2A7
increasing the cytoplasmic triglyceride pool in hepatocytes does not directly influence VLDL triglyceride or apoB production, overview
-
-
-
additional information
?
-
-
increased lipid synthesis has variable effects on diacylglycerol accumulation, overview, regulation of lipid biosynthesis in cultures tissues, changes in the endogenous activity of DAGAT is unlikely to affect oil accumulation in oil palm crops, overview
-
-
-
additional information
?
-
-
in castor bean DGAT2 is more likely to play a major role in seed triacylglycerol biosynthesis than DGAT1
-
-
-
additional information
?
-
-
mechanism for the degradation of the DGAT protein, overview
-
-
-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
Mn2+
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(2E)-1-[2,4-dihydroxy-6-methoxy-3-[(2R)-5-methyl-2-(1-methylethenyl)hex-4-en-1-yl]phenyl]-3-(2,4-dihydroxyphenyl)prop-2-en-1-one
-
IC50: 0.0098 mM
(2E,4Z,8E)-N-[9-(3,4-methylenedioxyphenyl)-2,4,8-nonatrienoyl]piperidine
-
IC50: 0.0298 mM, isolated from the extracts of fruits of Piper longum and Piper nigrum
(2R,3R)-2-(2,4-Dihydroxy-phenyl)-3,7-dihydroxy-8-((R)-5-hydroxy-2-isopropenyl-5-methyl-hexyl)-5-methoxy-chroman-4-one
-
i.e. kushenol H, prenylflavonoid from Sophora flavescens, 50% inhibition at 0.142 mM
(2R,3S)-2-(2,4-Dihydroxy-phenyl)-3,7-dihydroxy-8-((R)-5-hydroxy-2-isopropenyl-5-methyl-hexyl)-5-methoxy-chroman-4-one
-
i.e. kushenol K, prenylflavonoid from Sophora flavescens, 50% inhibition at 0.250 mM
(2S)-1-(3-[[(2S)-1-ethoxy-4-phenylbutan-2-yl]sulfamoyl]anilino)-4-methyl-1-oxopentan-2-yl 2,2-dimethylpropanoate
-
-
(2S)-1-[(1-[[(1S)-1-(3-cyclopropyl-1,2,4-oxadiazol-5-yl)-3-phenylpropyl]carbamoyl]cyclopentyl)amino]-4-methyl-1-oxopentan-2-yl 2,2-dimethylpropanoate
-
-
(trans-4-(4-[(5-benzyl-1,3,4-thiadiazol-2-yl)carbamoyl]phenyl)cyclohexyl)acetic acid
-
inhibitor displays good metabolic stability and high intestinal permeability values
(trans-4-(4-[(5-cyclopentylethyl-1,3,4-thiadiazol-2-yl)carbamoyl]phenyl)cyclohexyl)acetic acid
-
-
(trans-4-(4-[(5-cyclopentylmethyl-1,3,4-thiadiazol-2-yl)carbamoyl]phenyl)cyclohexyl)acetic acid
-
-
(trans-4-(4-[(5-[1-fluorobenzyl]-1,3,4-thiadiazol-2-yl)carbamoyl]phenyl)cyclohexyl)acetic acid
-
inhibitor displays good metabolic stability and high intestinal permeability values
(trans-4-(4-[(5-[2-chlorobenzyl]-1,3,4-thiadiazol-2-yl)carbamoyl]phenyl)cyclohexyl)acetic acid
-
inhibitor displays good metabolic stability and high intestinal permeability values
(trans-4-(4-[(5-[2-fluorobenzyl]-1,3,4-thiadiazol-2-yl)carbamoyl]phenyl)cyclohexyl)acetic acid
-
inhibitor displays good metabolic stability and high intestinal permeability values
(trans-4-[4-[(3-benzyl-1,2,4-oxadiazol-5-yl)carbamoyl]phenyl]cyclohexyl)acetic acid
-
-
(trans-4-[4-[(5-benzyl-1,2,4-oxadiazol-3-yl)carbamoyl]phenyl]cyclohexyl)acetic acid
-
-
(trans-4-[4-[(5-benzyl-1,3,4-thiadiazol-2-yl)carbamoyl]phenyl]cyclohexyl)acetic acid
-
-
1,2-diacyl-sn-glycerol
-
dicaprin, dimyristin, dipalmitin, high concentration
1-acyl-sn-glycero-3-phosphocholine
-
activation, at low concentrations, inhibition above 0.2 mM
1-O-Hexadecylpropanediol-3-phosphocholine
-
activation, low concentration, inhibitory at high concentration
1-[2,4-dihydroxy-3-(2-isopropenyl-5-methyl-hex-4-enyl)-6-methoxy-phenyl]-3-(2,4-dihydroxy-phenyl)-propenone
-
i.e. kuraridin, prenylflavonoid from Sophora flavescens, 50% inhibition at 0.099 mM
2-(2,4-dihydroxy-phenyl)-7-hydroxy-8-(2-isopropenyl-5-methyl-hex-4-enyl)-5-methoxy-chroman-4-one
-
i.e. kurarinone, prenylflavonoid from Sophora flavescens, 50% inhibition at 0.0109 mM
2-(2,4-dihydroxy-phenyl)-7-hydroxy-8-[2-(3-hydroxy-3-methyl-butyl)-3-methyl-but-3-enyl]-5-methoxy-chroman-4-one
-
i.e. kurarinol, prenylflavonoid from Sophora flavescens, 50% inhibition at 0.0086 mM
3-oxoolean-12-en-27-oic acid
-
the compound exhibits strong inhibition efficacy towards diacylglycerol acyltransferases 1 and 2, and acts competitively against oleoyl-CoA in vitro
7beta-(3-ethyl-cis-crotonoyloxy)-1alpha-(2-methylbutyryloxy)-3,14-dehydro-Z-notonipetranone
aceriphyllic acid A
-
the compound exhibits strong inhibition efficacy towards diacylglycerol acyltransferases 1 and 2, and acts competitively against oleoyl-CoA in vitro
cis-4-(4-[[5-(cyclopentylamino)-1,3,4-thiadiazol-2-yl]carbamoyl]phenoxy)cyclohexane-1-carboxylic acid
-
-
cis-4-([5-[(5-benzyl-1,3,4-thiadiazol-2-yl)carbamoyl]pyridin-2-yl]oxy)cyclohexane-1-carboxylic acid
-
-
cis-4-[4-([3-[(3,5-difluorophenyl)methyl]-1,2,4-oxadiazol-5-yl]carbamoyl)phenoxy]cyclohexane-1-carboxylic acid
-
-
cis-4-[4-([5-[(3,5-difluorophenyl)methyl]-1,3,4-thiadiazol-2-yl]carbamoyl)phenoxy]cyclohexane-1-carboxylic acid
-
-
cis-4-[4-([5-[(cyclopentyloxy)methyl]-1,3,4-thiadiazol-2-yl]carbamoyl)phenoxy]cyclohexane-1-carboxylic acid
-
-
cis-4-[4-[(3-benzyl-1,2,4-oxadiazol-5-yl)carbamoyl]phenoxy]cyclohexane-1-carboxylic acid
-
-
cis-4-[4-[(5-anilino-1,3,4-thiadiazol-2-yl)carbamoyl]phenoxy]cyclohexane-1-carboxylic acid
-
-
cis-4-[4-[(5-benzyl-1,3,4-thiadiazol-2-yl)carbamoyl]phenoxy]cyclohexane-1-carboxylic acid
-
-
cis-4-[[5-([5-[(3-chlorophenyl)methyl]-1,3,4-thiadiazol-2-yl]carbamoyl)pyridin-2-yl]oxy]cyclohexane-1-carboxylic acid
-
-
dehydropipernonaline
-
noncompetitive inhibition, IC50: 0.0212 mM, isolated from the extracts of fruits of Piper longum and Piper nigrum
lysophosphatidylcholine
-
activation at low concentrations, optimum at 0.075 mM, inhibitory above 0.2 mM
methyl 1-benzyl-3-[(furan-3-carbonyl)amino]-5-[(2-methylbutyl)amino]-1H-pyrrolo[2,3-b]pyridine-2-carboxylate
-
-
methyl 1-benzyl-3-[(furan-3-carbonyl)amino]-5-[[(thiophen-3-yl)methyl]amino]-1H-pyrrolo[2,3-b]pyridine-2-carboxylate
-
-
methyl 3-benzamido-1-[(furan-2-yl)methyl]-5-[(2-methylbutyl)amino]-1H-pyrrolo[2,3-b]pyridine-2-carboxylate
-
-
methyl 3-[(furan-2-carbonyl)amino]-1-(3-methylbutyl)-5-[(pentan-3-yl)amino]-1H-pyrrolo[2,3-b]pyridine-2-carboxylate
-
-
methyl 3-[(furan-3-carbonyl)amino]-1-(2-methylpropyl)-5-[(pentan-3-yl)amino]-1H-pyrrolo[2,3-b]pyridine-2-carboxylate
-
-
methyl 5-[(2,3-dihydro-1H-inden-2-yl)amino]-3-[(furan-3-carbonyl)amino]-1-(2-methylpropyl)-1H-pyrrolo[2,3-b]pyridine-2-carboxylate
-
-
methyl 5-[[(4-acetamidophenyl)methyl]amino]-1-benzyl-3-[(furan-3-carbonyl)amino]-1H-pyrrolo[2,3-b]pyridine-2-carboxylate
-
-
methyl 5-[[(furan-3-yl)methyl]amino]-1-(2-methylpropyl)-3-(2-phenylacetamido)-1H-pyrrolo[2,3-b]pyridine-2-carboxylate
-
-
N-(1-[[5-tert-butyl-1-(4-fluorophenyl)-1H-1,2,4-triazol-3-yl]amino]-2-methyl-1-oxopropan-2-yl)-4-fluorobenzamide
-
-
N-(4,5-dihydronaphtho[1,2-d]thiazol-2-yl)-2-(3,4-dimethoxy phenyl)acetamide
selective inhibitor of isoform DGAT2 when used at low concentrations; selective inhibitor of isoform DGAT2 when used at low concentrations
N-(5-benzyl-1,3,4-thiadiazol-2-yl)-4-(trans-4-[[2-(dimethylamino)ethyl]carbamoyl]cyclohexyl)benzamide
-
-
N-(5-benzyl-1,3,4-thiadiazol-2-yl)-4-(trans-4-[[2-(morpholin-4-yl)ethyl]carbamoyl]cyclohexyl)benzamide
-
-
N-(5-benzyl-1,3,4-thiadiazol-2-yl)-4-[trans-4-[(2,3-dihydroxypropyl)carbamoyl]cyclohexyl]benzamide
-
-
N-(5-benzyl-1,3,4-thiadiazol-2-yl)-4-[trans-4-[(2-hydroxy-2-methylpropyl)carbamoyl]cyclohexyl]benzamide
-
-
N-(5-benzyl-1,3,4-thiadiazol-2-yl)-4-[trans-4-[(2-methoxyethyl)carbamoyl]cyclohexyl]benzamide
-
-
N-[(2S)-1-ethoxy-4-phenylbutan-2-yl]-1-[2-(tricyclo[3.3.1.1~3,7~]decan-2-yl)acetamido]cyclopentane-1-carboxamide
-
-
N-[1,5-bis(4-fluorophenyl)-1H-1,2,4-triazol-3-yl]-2-methyl-N~2~-[1-(trifluoromethyl)cyclopropane-1-carbonyl]alaninamide
-
-
N-[1-(4-fluoro-2-methylphenyl)-5-(4-fluorophenyl)-1H-1,2,4-triazol-3-yl]-2-methyl-N~2~-[1-(trifluoromethyl)cyclopropane-1-carbonyl]alaninamide
-
-
N-[1-(4-fluorophenyl)-5-[2-(4-fluorophenyl)propan-2-yl]-1H-1,2,4-triazol-3-yl]-2-methyl-N~2~-[1-(trifluoromethyl)cyclopropane-1-carbonyl]alaninamide
-
-
N-[5-tert-butyl-1-(4-fluorophenyl)-1H-1,2,4-triazol-3-yl]-2-methyl-N~2~-(1-methylcyclopropane-1-carbonyl)alaninamide
-
-
N-[5-tert-butyl-1-(4-fluorophenyl)-1H-1,2,4-triazol-3-yl]-2-methyl-N~2~-[1-(trifluoromethyl)cyclopropane-1-carbonyl]alaninamide
-
-
pipernonaline
-
IC50: 0.0372 mM, isolated from the extracts of fruits of Piper longum and Piper nigrum
piperrolein B
-
IC50: 0.0201 mM, isolated from the extracts of fruits of Piper longum and Piper nigrum
retrofractamide C
-
IC50: 0.9 mM, above, isolated from the extracts of fruits of Piper longum and Piper nigrum
tert-butyl (2S)-2-[[1-([(2S)-2-[(2,2-dimethylpropanoyl)oxy]-4-methylpentanoyl]amino)cyclopentane-1-carbonyl]amino]-4-phenylbutanoate
-
-
trans-4-[4-([5-[(3,5-difluorophenyl)methyl]-1,3,4-thiadiazol-2-yl]carbamoyl)phenoxy]cyclohexane-1-carboxylic acid
-
-
trans-4-[4-[(5-benzyl-1,3,4-thiadiazol-2-yl)carbamoyl]phenoxy]cyclohexane-1-carboxylic acid
-
-
Trifluoperazine
-
0.5 mM, in the presence of suboptimal phosphatidic acid concentration
[trans-4-(4-[[5-(2-cyclopentylethyl)-1,3,4-thiadiazol-2-yl]carbamoyl]phenyl)cyclohexyl]acetic acid
-
-
[trans-4-[4-([5-[(3,5-difluorophenyl)methyl]-1,3,4-thiadiazol-2-yl]carbamoyl)phenyl]cyclohexyl]acetic acid
-
-
[trans-4-[4-([5-[(4-fluorophenyl)methyl]-1,3,4-oxadiazol-2-yl]carbamoyl)phenyl]cyclohexyl]acetic acid
-
-
[trans-4-[4-([5-[(4-fluorophenyl)methyl]-1,3,4-thiadiazol-2-yl]carbamoyl)phenyl]cyclohexyl]acetic acid
-
-
[trans-4-[4-([5-[(cyclopentyloxy)methyl]-1,3,4-thiadiazol-2-yl]carbamoyl)phenyl]cyclohexyl]acetic acid
-
-
[trans-4-[4-([5-[2-(oxolan-2-yl)ethyl]-1,3,4-thiadiazol-2-yl]carbamoyl)phenyl]cyclohexyl]acetic acid
-
-
[trans-4-[4-([5-[2-(oxolan-3-yl)ethyl]-1,3,4-thiadiazol-2-yl]carbamoyl)phenyl]cyclohexyl]acetic acid
-
-
5,5'-dithio-bis(2-nitrobenzoic acid)
-
i.e. DTNB, complete inhibition, DTT protects
7beta-(3-ethyl-cis-crotonoyloxy)-1alpha-(2-methylbutyryloxy)-3,14-dehydro-Z-notonipetranone
-
-
7beta-(3-ethyl-cis-crotonoyloxy)-1alpha-(2-methylbutyryloxy)-3,14-dehydro-Z-notonipetranone
-
-
betulinic acid
-
a lupane-type triterpenoid, isolated from methanolic extracts of Alnus hirsuta, inhibition of Hep-G2 cell triacyglycerol biosynthesis
betulinic acid
-
a lupane-type triterpenoid, isolated from methanolic extracts of Alnus hirsuta, noncompetitive inhibition, IC50: 0.0096 mM
bovine serum albumin
-
binds palmitoyl-CoA and decreases activity towards palmitoyl-CoA, but not butyryl-CoA
-
CaCl2
-
2 mM, inhibits purified enzyme but not activity in lipid body fraction
DTT