Literature summary for 2.3.1.20 extracted from

Chhikara, S.; Abdullah, H.M.; Akbari, P.; Schnell, D.; Dhankher, O.P.
Engineering Camelina sativa (L.) Crantz for enhanced oil and seed yields by combining diacylglycerol acyltransferase1 and glycerol-3-phosphate dehydrogenase expression (2018), Plant Biotechnol. J., 16, 1034-1045 .

Application

Application	Comment	Organism
biofuel production	combining the overexpression of TAG biosynthetic genes, DGAT1 and GPD1, appears to be a positive strategy to achieve a synergistic effect on the flux through the TAG synthesis pathway, and thereby further increase the oil yield of Camelina sativa, application of genetic engineering approaches to boost the metabolic flux of carbon into seed oils	Arabidopsis thaliana
biotechnology	combining the overexpression of TAG biosynthetic genes, DGAT1 and GPD1, appears to be a positive strategy to achieve a synergistic effect on the flux through the TAG synthesis pathway, and thereby further increase the oil yield of Camelina sativa, application of genetic engineering approaches to boost the metabolic flux of carbon into seed oils	Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment)	Organism
gene DGAT1, seed-specific co-overexpression of AtDGAT1 and ScGPD1 codon-optimized genes in Camelina sativa cv. Suneson, increasing seed mass, seed size and seed yield in the transgenic plants, recombinant co-expression also with mutant DGATm, the glycinin promoter from Glycine max is selected to drive the expression of DGAT1, transfection using the Agrobacterium tumefaciens strain GV3101 method. Quantitative real-time PCR enzyme expression analysis and phenotypes, overview	Arabidopsis thaliana

Cloned (Comment)

Organism

gene DGAT1, seed-specific co-overexpression of AtDGAT1 and ScGPD1 codon-optimized genes in Camelina sativa cv. Suneson, increasing seed mass, seed size and seed yield in the transgenic plants, recombinant co-expression also with mutant DGATm, the glycinin promoter from Glycine max is selected to drive the expression of DGAT1, transfection using the Agrobacterium tumefaciens strain GV3101 method. Quantitative real-time PCR enzyme expression analysis and phenotypes, overview

Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
additional information	engineering transgenic Camelina sativa plants for enhanced oil and seed yields by combining heterologous expression of Arabidopsis thaliana diacylglycerol acyltransferase1 (DGAT1) and Saccharomyces cerevisiae cytosolic glycerol-3-phosphate dehydrogenase (GPD1) genes under the control of seed-specific promoters. Plants co-expressing DGAT1 and GPD1 exhibit up to 13% higher seed oil content and up to 52% increase in seed mass compared to wild-type plants. Further, DGAT1- and GDP1-coexpressing lines show significantly higher seed and oil yields on a dry weight basis than the wild-type controls or plants expressing DGAT1 and GPD1 alone. The oil harvest index (g oil per g total dry matter) for DGTA1- and GPD1-co-expressing lines is almost twofold higher as compared to wild-type and the lines expressing DGAT1 and GPD1 alone. Evaluation of the effect of stacking the two genes on achieving a synergistic effect on the flux through the TAG synthesis pathway, and thereby further increasing the oil yield. GDP1 and DGAT1 overexpression has no effect on seed germination and early seedling growth	Arabidopsis thaliana
S205A	site-directed mutagenesis, mutant DGAT1m is less effective compared to wild-type in increasing seed mass, seed size and seed yield in the transgenic Camelina sativa plants when coexpressed with yeast GPD1	Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
acyl-CoA + 1,2-diacyl-sn-glycerol	Arabidopsis thaliana	-	CoA + 1,2,3-triacylglycerol	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q9SLD2	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acyl-CoA + 1,2-diacyl-sn-glycerol	-	Arabidopsis thaliana	CoA + 1,2,3-triacylglycerol	-	?

Synonyms

Synonyms	Comment	Organism
acyl-CoA-dependent diacylglycerol acyltransferase	-	Arabidopsis thaliana
DGAT1	-	Arabidopsis thaliana
diacylglycerol acyltransferase1	-	Arabidopsis thaliana

General Information

General Information	Comment	Organism
metabolism	DGAT1 enzyme is evidenced to be a major determining factor for oil quantity and fatty acid composition of seed oils in several crops	Arabidopsis thaliana