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Literature summary for 2.3.1.20 extracted from

  • Jeennor, S.; Veerana, M.; Anantayanon, J.; Panchanawaporn, S.; Chutrakul, C.; Laoteng, K.
    Diacylglycerol acyltransferase 2 of Mortierella alpina with specificity on long-chain polyunsaturated fatty acids a potential tool for reconstituting lipids with nutritional value (2017), J. Biotechnol., 263, 45-51 .
    View publication on PubMed
Search for more literature for 2.3.1.20

Cloned(Commentary)

Cloned (Comment) Organism
gene DGAT2B, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, subcloning in Escherichia coli strain DH5alpha, recombinant expression of wild-type (MaDGAT2) Mortierella alpina gene in and complementation of Saccharomyces cerevisiae strain H1246 defective in synthesis of neutral lipid, lipid analysis of the lipid content of the recombinant culture harboring MaDGAT2 gene Mortierella alpina
gene mMaDGAT2B, subcloning in Escherichia coli strain DH5alpha, synthetic gene after codon-optimization, recombinant expression of codon-optimized (mMaDGAT2) Mortierella alpina gene in and complementation of Saccharomyces cerevisiae strain H1246 defective in synthesis of neutral lipid, lipid analysis of the lipid content of the recombinant culture harboring mMaDGAT2 gene Mortierella alpina

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-CoA + 1,2-diacyl-sn-glycerol Mortierella alpina
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 CoA + 1,2,3-triacylglycerol
-
 ?
acyl-CoA + 1,2-diacyl-sn-glycerol Mortierella alpina ATCC 32222
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 CoA + 1,2,3-triacylglycerol
-
 ?

Organism

Organism UniProt Comment Textmining
Mortierella alpina A0A2D2CI94
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Mortierella alpina KY859195 synthetic gene, codon-optimized, following the sequence (UniProt A0A2D2CI94) of the wild-type gene of MaDGAT2
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Mortierella alpina ATCC 32222 A0A2D2CI94
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + 1,2-diacyl-sn-glycerol
-
 Mortierella alpina CoA + 1,2,3-triacylglycerol
-
 ?
acyl-CoA + 1,2-diacyl-sn-glycerol
-
 Mortierella alpina ATCC 32222 CoA + 1,2,3-triacylglycerol
-
 ?
arachidonoyl-CoA + 1,2-diacyl-sn-glycerol
-
 Mortierella alpina CoA + 1,2-diacyl-3-arachidnoylglycerol
-
 ?
arachidonoyl-CoA + 1,2-diacyl-sn-glycerol
-
 Mortierella alpina ATCC 32222 CoA + 1,2-diacyl-3-arachidnoylglycerol
-
 ?
dihomo gamma-linolenoyl-CoA + 1,2-diacyl-sn-glycerol
-
 Mortierella alpina CoA + 1,2-diacyl-3-dihomo-gamma-linolenoylglycerol
-
 ?
dihomo gamma-linolenoyl-CoA + 1,2-diacyl-sn-glycerol
-
 Mortierella alpina ATCC 32222 CoA + 1,2-diacyl-3-dihomo-gamma-linolenoylglycerol
-
 ?
gamma-linolenoyl-CoA + 1,2-diacyl-sn-glycerol
-
 Mortierella alpina CoA + 1,2-diacyl-3-gamma-linolenoylglycerol
-
 ?
gamma-linolenoyl-CoA + 1,2-diacyl-sn-glycerol
-
 Mortierella alpina ATCC 32222 CoA + 1,2-diacyl-3-gamma-linolenoylglycerol
-
 ?
linoleoyl-CoA + 1,2-diacyl-sn-glycerol
-
 Mortierella alpina CoA + 1,2-diacyl-3-linoleoylglycerol
-
 ?
additional information substrate specificity study, overview. The enzyme expressed in yeast mutant cells has a broad specificity on saturated and unsaturated fatty acid substrates for esterification into triacylglycerol (TAG). TAG fraction of the codon-optimized mMaDGAT2 transformant contains endogenous saturated fatty acids (C16:0 and C18:0) and monounsaturated fatty acids (palmitoleic acid; C16:1DELTA9 and oleic acid; C18:1DELTA9) as major proportions, which is a usual fatty acid profile of Saccharomyces cerevisiae. Medium-chain saturated fatty acids with 15- to 18-carbon atoms are substrates for mMaDGAT2. n-6 polyunsaturated fatty acids (PUFAs), linoleic acid, gamma-linolenic acid, dihomo gamma-linolenic and arachidonic acid, are highly accumulated in TAG fraction of the mMaDGAT2 culture as compared to the n-3 PUFAs (alpha-linolenic and docosahexaenoic acid) except for C20:5 n-3 (eicosapentaenoic acid). Similar results are also obtained in the cultures fed with mixtures of n-3 PUFAs, demonstrating that mMaDGAT2 prefers eicosapentaenoic acid over gamma-linolenic and docosahexaenoic acid, respectively Mortierella alpina ?
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-
additional information substrate specificity study, overview. The recombinant enzyme expressed in yeast mutant cells has a broad specificity on saturated and unsaturated fatty acid substrates with different position and number of double bonds in their acyl chains for esterification into triacylglycerol (TAG). The n-6 polyunsaturated fatty acids (PUFAs) with 18 and 20 carbon atoms, including linoleic acid, gamma-linolenic acid, dihomo gamma-linolenic acid (DGLA ) and arachidonic acid are incorporated into the TAG fraction in recombinantly expressing yeast cells. Among n-3 PUFAs tested, the MaDGAT2 enzyme prefers eicosapentaenoic acid (EPA) substrate regarding its highly proportion found in the TAG fraction. DGLA and EPA can be efficiently used as substrates by MaDGAT2 Mortierella alpina ?
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-
additional information substrate specificity study, overview. The recombinant enzyme expressed in yeast mutant cells has a broad specificity on saturated and unsaturated fatty acid substrates with different position and number of double bonds in their acyl chains for esterification into triacylglycerol (TAG). The n-6 polyunsaturated fatty acids (PUFAs) with 18 and 20 carbon atoms, including linoleic acid, gamma-linolenic acid, dihomo gamma-linolenic acid (DGLA ) and arachidonic acid are incorporated into the TAG fraction in recombinantly expressing yeast cells. Among n-3 PUFAs tested, the MaDGAT2 enzyme prefers eicosapentaenoic acid (EPA) substrate regarding its highly proportion found in the TAG fraction. DGLA and EPA can be efficiently used as substrates by MaDGAT2 Mortierella alpina ATCC 32222 ?
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-

Synonyms

Synonyms Comment Organism
DGAT2b
-
 Mortierella alpina
diacylglycerol acyltransferase
-
 Mortierella alpina
MaDGAT2
-
 Mortierella alpina
mMaDGAT2
-
 Mortierella alpina

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 in vivo assay at Mortierella alpina

General Information

General Information Comment Organism
physiological function the MaDGAT2 gene encodes diacylglycerol acyltransferase 2, which is involved in the biosynthesis of neutral lipids and formation of lipid particle Mortierella alpina