Literature summary for 2.3.1.20 extracted from

Li, D.W.; Cen, S.Y.; Liu, Y.H.; Balamurugan, S.; Zheng, X.Y.; Alimujiang, A.; Yang, W.D.; Liu, J.S.; Li, H.Y.
A type 2 diacylglycerol acyltransferase accelerates the triacylglycerol biosynthesis in heterokont oleaginous microalga Nannochloropsis oceanica (2016), J. Biotechnol., 229, 65-71 .

Search for more literature for 2.3.1.20

Cloned(Commentary)

Cloned (Comment)	Organism
gene DGAT2, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons, and phylogenetic analysis, recombinant overexpression of FLAG-tagged enzyme DGAT2 in Nannochloropsis oceanica cells, real-time quantitative PCR expression analysis	Nannochloropsis oceanica

Protein Variants

Protein Variants	Comment	Organism
additional information	generation of DGAT2 overexpressing cells of Nannochloropsis oceanica, expression analysis and lipid content determination, overview. The introduced transgene DGAT2 is successfully integrated,transcribed and translated in the transformed algal cells. The overexpressed DGAT2 does not have apparent effects on the growth and biomass accumulation of the engineered microalgae, analysis of neutral lipid content. There is a variation in terms of fatty acid composition between the engineered and wild-type cells. Saturated fatty acid (SFAs) content is significantly increased by 53.1% in engineered lines compared to wild-type. On the other hand,monounsaturated fatty acids (MUFAs) decrease by 52.9% in engineered cells. Similarly, polyunsaturated fatty acids (PUFAs) show an apparent decrease of 74.6%, including arachidonic acid (C20:4) and EPA (C20:5) in engineered cells	Nannochloropsis oceanica

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
acyl-CoA + 1,2-diacyl-sn-glycerol	Nannochloropsis oceanica	-	CoA + 1,2,3-triacylglycerol	-	?
acyl-CoA + 1,2-diacyl-sn-glycerol	Nannochloropsis oceanica CCAP 849/10	-	CoA + 1,2,3-triacylglycerol	-	?

Organism

Organism	UniProt	Comment	Textmining
Nannochloropsis oceanica	A0A191M6B9	formerly CCMP1779	-
Nannochloropsis oceanica CCAP 849/10	A0A191M6B9	formerly CCMP1779	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acyl-CoA + 1,2-diacyl-sn-glycerol	-	Nannochloropsis oceanica	CoA + 1,2,3-triacylglycerol	-	?
acyl-CoA + 1,2-diacyl-sn-glycerol	-	Nannochloropsis oceanica CCAP 849/10	CoA + 1,2,3-triacylglycerol	-	?

Subunits

Subunits	Comment	Organism
?	x * 43000, recombinant FLAG-tagged enzyme, SDS-PAGE	Nannochloropsis oceanica

Synonyms

Synonyms	Comment	Organism
2 diacylglycerol acyltransferase	-	Nannochloropsis oceanica
DGAT2	-	Nannochloropsis oceanica

General Information

General Information	Comment	Organism
physiological function	the type 2 diacylglycerol acyltransferase accelerates the triacylglycerol biosynthesis in heterokont oleaginous microalga Nannochloropsis oceanica	Nannochloropsis oceanica

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Release 2023.1 (January 2023)