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Literature summary for 2.3.1.20 extracted from
- Identification and characterization of diacylglycerol acyltransferase in oleaginous yeast Rhodosporidium toruloides (2016), Am. J. Biochem. Biotechnol., 12, 230-240 .
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| RtDGATa, sequence comparisons and phylogenetic analysis, recombinant expression of isozyme RtDGATa in Saccharomyces cerevisiae TAG-deficient quadruple mutant strain H1246, subcloning in Escherichia coli Top10 cells. The expression of RtDGATa does not alter the TAG content in Saccharomyces cerevisiae strain H1246 | Rhodotorula toruloides |
| RtDGATb, sequence comparisons and phylogenetic analysis, recombinant expression of isozyme RtDGATb in Saccharomyces cerevisiae TAG-deficient quadruple mutant strain H1246, subcloning in Escherichia coli Top10 cells. The expression of RtDGATb completely resumes TAG biosynthesis in Saccharomyces cerevisiae strain H1246 | Rhodotorula toruloides |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Rhodotorula toruloides | 16020 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + 1,2-diacyl-sn-glycerol | Rhodotorula toruloides | - |
CoA + 1,2,3-triacylglycerol | - |
? |  |
| acyl-CoA + 1,2-diacyl-sn-glycerol | Rhodotorula toruloides CGMCC 2.1389 | - |
CoA + 1,2,3-triacylglycerol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodotorula toruloides | A0A2Z6EYT1 | - |
- |
| Rhodotorula toruloides | A0A511K7D9 | - |
- |
| Rhodotorula toruloides CGMCC 2.1389 | A0A2Z6EYT1 | - |
- |
| Rhodotorula toruloides CGMCC 2.1389 | A0A511K7D9 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + 1,2-diacyl-sn-glycerol | - |
Rhodotorula toruloides | CoA + 1,2,3-triacylglycerol | - |
? | |
| acyl-CoA + 1,2-diacyl-sn-glycerol | isozyme RtDGATb prefers unsaturated fatty acids over saturated fatty acids, but not C18:3 | Rhodotorula toruloides | CoA + 1,2,3-triacylglycerol | - |
? | |
| acyl-CoA + 1,2-diacyl-sn-glycerol | RtDGATa has obvious preference for monounsaturated fatty acids. RtDGATa is active with C16:1 and C18:1 and shows poor activity for C16:0, C18:0, C18:2 and C18:3 | Rhodotorula toruloides | CoA + 1,2,3-triacylglycerol | - |
? | |
| acyl-CoA + 1,2-diacyl-sn-glycerol | - |
Rhodotorula toruloides CGMCC 2.1389 | CoA + 1,2,3-triacylglycerol | - |
? | |
| acyl-CoA + 1,2-diacyl-sn-glycerol | RtDGATa has obvious preference for monounsaturated fatty acids. RtDGATa is active with C16:1 and C18:1 and shows poor activity for C16:0, C18:0, C18:2 and C18:3 | Rhodotorula toruloides CGMCC 2.1389 | CoA + 1,2,3-triacylglycerol | - |
? | |
| acyl-CoA + 1,2-diacyl-sn-glycerol | isozyme RtDGATb prefers unsaturated fatty acids over saturated fatty acids, but not C18:3 | Rhodotorula toruloides CGMCC 2.1389 | CoA + 1,2,3-triacylglycerol | - |
? | |
| additional information | isozyme substrate specificities, overview | Rhodotorula toruloides | ? | - |
- |
|
| additional information | isozyme substrate specificities, overview | Rhodotorula toruloides CGMCC 2.1389 | ? | - |
- |
|
| oleoyl-CoA + 1,2-diacyl-sn-glycerol | - |
Rhodotorula toruloides | CoA + 1,2-diacyl-3-oleoylglycerol | - |
? | |
| oleoyl-CoA + 1,2-diacyl-sn-glycerol | low activity | Rhodotorula toruloides | CoA + 1,2-diacyl-3-oleoylglycerol | - |
? | |
| oleoyl-CoA + 1,2-diacyl-sn-glycerol | - |
Rhodotorula toruloides CGMCC 2.1389 | CoA + 1,2-diacyl-3-oleoylglycerol | - |
? | |
| oleoyl-CoA + 1,2-diacyl-sn-glycerol | low activity | Rhodotorula toruloides CGMCC 2.1389 | CoA + 1,2-diacyl-3-oleoylglycerol | - |
? | |
| palmitoleoyl-CoA + 1,2-diacyl-sn-glycerol | best substrate | Rhodotorula toruloides | CoA + 1,2-diacyl-3-palmitoleoylglycerol | - |
? | |
| palmitoleoyl-CoA + 1,2-diacyl-sn-glycerol | low activity | Rhodotorula toruloides | CoA + 1,2-diacyl-3-palmitoleoylglycerol | - |
? | |
| palmitoleoyl-CoA + 1,2-diacyl-sn-glycerol | best substrate | Rhodotorula toruloides CGMCC 2.1389 | CoA + 1,2-diacyl-3-palmitoleoylglycerol | - |
? | |
| palmitoleoyl-CoA + 1,2-diacyl-sn-glycerol | low activity | Rhodotorula toruloides CGMCC 2.1389 | CoA + 1,2-diacyl-3-palmitoleoylglycerol | - |
? | |
| palmitoyl-CoA + 1,2-diacyl-sn-glycerol | high activity | Rhodotorula toruloides | CoA + 1,2-diacyl-3-palmitoylglycerol | - |
? | |
| stearoyl-CoA + 1,2-diacyl-sn-glycerol | high activity | Rhodotorula toruloides | CoA + 1,2-diacyl-3-stearoylglycerol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DGA1 | - |
Rhodotorula toruloides |
| DGA2 | - |
Rhodotorula toruloides |
| DGAT | - |
Rhodotorula toruloides |
| diacylglycerol acyltransferase | - |
Rhodotorula toruloides |
| RtDGATa | - |
Rhodotorula toruloides |
| RtDGATb | - |
Rhodotorula toruloides |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the genome of oleaginous yeast Rhodosporidium toruloides contains two putative DGAT genes, RtDGATa and RtDGATb, which share little conserved amino acid coding sequence with each other. Phylogeny tree analysis shows that RtDGATa belongs to DGAT1 family and RtDGATb belongs to DGAT2 family | Rhodotorula toruloides |
| physiological function | the expression of isozyme RtDGATa shows non-involvement in triacylglycerol (TAG) accumulation according to its mRNA expression level in Rhodosporidium toruloides | Rhodotorula toruloides |
| physiological function | the expression pattern of isozyme RtDGATb is related to the process of fatty acid biosynthesis, suggesting that RtDGATb plays an important role in lipid accumulation in Rhodosporidium toruloides | Rhodotorula toruloides |
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