Cloned (Comment) | Organism |
---|---|
expression as His-tagged enzyme in Escherichia coli strain BL21 (DE3), phylogenetic analysis | Arachis hypogaea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Arachis hypogaea | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Arachis hypogaea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
x * 41000, recombinant His-tagged enzyme, SDS-PAGE | Arachis hypogaea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-diacylglycerol + acyl-CoA | Arachis hypogaea | the enzyme catalyzes the final step in triacylglycerol biosynthesis that acylates diacylglycerol to triacylglycerols | triacylglycerol + CoA | - |
? | |
1,2-diacylglycerol + acyl-CoA | Arachis hypogaea JL 24 | the enzyme catalyzes the final step in triacylglycerol biosynthesis that acylates diacylglycerol to triacylglycerols | triacylglycerol + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arachis hypogaea | - |
- |
- |
Arachis hypogaea | Q2KP14 | - |
- |
Arachis hypogaea JL 24 | Q2KP14 | - |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 1724fold from developing cotyldons by hydrophobic interaction and adsorption chromatography, followed by gel filtration and heparin affinity chromatography to homogeneity | Arachis hypogaea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | developing | Arachis hypogaea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
substrate specificity of native and recombinant enzyme | Arachis hypogaea |
0.0071 | - |
purified native enzyme | Arachis hypogaea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-diacylglycerol + acyl-CoA | the enzyme catalyzes the final step in triacylglycerol biosynthesis that acylates diacylglycerol to triacylglycerols | Arachis hypogaea | triacylglycerol + CoA | - |
? | |
1,2-diacylglycerol + acyl-CoA | the enzyme catalyzes the final step in triacylglycerol biosynthesis that acylates diacylglycerol to triacylglycerols | Arachis hypogaea JL 24 | triacylglycerol + CoA | - |
? | |
1,2-dipalmitoyl-sn-glycerol + oleoyl-CoA | - |
Arachis hypogaea | 1,2-dipalmitoyl-3-oleoyl-sn-glycerol + CoA | - |
? | |
1,2-dipalmitoyl-sn-glycerol + palmitoyl-CoA | - |
Arachis hypogaea | tripalmitin + CoA | - |
? | |
1,2-dipalmitoyl-sn-glycerol + stearoyl-CoA | - |
Arachis hypogaea | 1,2-dipalmitoyl-3-stearoyl-sn-glycerol + CoA | - |
? | |
additional information | substrate specificity of native and recombinant enzyme, the native and the recombinant enzyme do not show wax ester synthase activity in contrast to other DGATs, no activity with hexadecanol, glycerol-3-phosphate, monoacylglycerol, lysophosphatidic acid, and lysophosphatidylcholine, oleoyl-CoA is the preferred acyl donor as compared to palmitoyl- and stearoyl-CoAs | Arachis hypogaea | ? | - |
? | |
additional information | substrate specificity of native and recombinant enzyme, the native and the recombinant enzyme do not show wax ester synthase activity in contrast to other DGATs, no activity with hexadecanol, glycerol-3-phosphate, monoacylglycerol, lysophosphatidic acid, and lysophosphatidylcholine, oleoyl-CoA is the preferred acyl donor as compared to palmitoyl- and stearoyl-CoAs | Arachis hypogaea JL 24 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 41000, recombinant His-tagged enzyme, SDS-PAGE | Arachis hypogaea |
Synonyms | Comment | Organism |
---|---|---|
acyl-CoA:diacylglycerol acyltransferase | - |
Arachis hypogaea |
DGAT | - |
Arachis hypogaea |
diacylglycerol acyltransferase | - |
Arachis hypogaea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Arachis hypogaea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Arachis hypogaea |