BRENDA - Enzyme Database
show all sequences of 2.2.1.5

Demonstration of 2-oxoglutarate:glyoxylate carboligase activity in Tetrahymena pyriformis

Zaror-Behrens, G.; Mavrides, C.; Can. J. Biochem. 51, 1505-1508 (1973)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
-
Tetrahymena pyriformis
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Tetrahymena pyriformis
5739
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Tetrahymena pyriformis
Mn2+
substitutes for Mg2+
Tetrahymena pyriformis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Tetrahymena pyriformis
-
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Tetrahymena pyriformis
0.128
-
maximum, cell-cycle-dependent
Tetrahymena pyriformis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxoglutarate + glyoxylate
-
395926
Tetrahymena pyriformis
2-hydroxy-3-oxohexandioate + CO2
i. e. 2-hydroxy-3-oxoadipic acid
395926
Tetrahymena pyriformis
-
2-oxoglutarate + glyoxylate
-
395926
Tetrahymena pyriformis
2-hydroxy-3-oxohexandioate + CO2
further spontaneous decarboxylation in the presence of acid
395926
Tetrahymena pyriformis
-
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
enzyme assay at
Tetrahymena pyriformis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
enzyme assay at
Tetrahymena pyriformis
Cofactor
Cofactor
Commentary
Organism
Structure
thiamine diphosphate
-
Tetrahymena pyriformis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
thiamine diphosphate
-
Tetrahymena pyriformis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
-
Tetrahymena pyriformis
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Tetrahymena pyriformis
5739
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Tetrahymena pyriformis
Mn2+
substitutes for Mg2+
Tetrahymena pyriformis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Tetrahymena pyriformis
0.128
-
maximum, cell-cycle-dependent
Tetrahymena pyriformis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxoglutarate + glyoxylate
-
395926
Tetrahymena pyriformis
2-hydroxy-3-oxohexandioate + CO2
i. e. 2-hydroxy-3-oxoadipic acid
395926
Tetrahymena pyriformis
-
2-oxoglutarate + glyoxylate
-
395926
Tetrahymena pyriformis
2-hydroxy-3-oxohexandioate + CO2
further spontaneous decarboxylation in the presence of acid
395926
Tetrahymena pyriformis
-
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
enzyme assay at
Tetrahymena pyriformis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
enzyme assay at
Tetrahymena pyriformis
Other publictions for EC 2.2.1.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736420
Balakrishnan
Influence of allosteric regula ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Biol. Chem.
288
21688-21702
2013
-
-
1
-
-
-
2
2
-
1
-
2
-
161
-
-
1
-
-
-
-
-
2
-
-
-
-
2
1
-
-
1
2
-
-
-
-
1
1
-
-
-
-
2
2
2
-
1
-
2
-
-
-
1
-
-
-
-
2
-
-
-
-
2
1
-
-
-
-
-
-
-
2
2
736654
Patel
-
Investigation of the donor and ...
Escherichia coli
J. Mol. Catal. B
98
42-45
2013
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
708099
de Carvalho
Activity-based metabolomic pro ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Chem. Biol.
17
323-332
2010
-
-
-
-
-
-
-
-
-
1
-
4
-
3
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
719051
Zhao
A protecting group-free synthe ...
Mycobacterium tuberculosis
Bioorg. Med. Chem. Lett.
20
6472-6474
2010
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657709
Dills
-
High pressure liquid chromatog ...
Bos taurus
Biochem. Arch.
15
279-284
1999
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
395931
Rattan
Oxalate metabolism in magnesiu ...
Rattus norvegicus
Magnes. Res.
6
127-133
1993
-
1
-
-
-
-
-
-
-
1
-
-
-
3
-
-
-
-
-
2
-
-
1
-
1
-
-
-
1
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
395936
Bats
Investigations into the effect ...
Rattus norvegicus
Nephron
57
460-469
1991
-
-
-
-
-
-
-
2
1
-
-
1
-
2
-
-
1
-
-
-
1
1
2
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
1
-
-
1
-
-
-
1
-
-
1
1
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
395933
Sidhu
Oxalate metabolism in thiamine ...
Rattus norvegicus
Ann. Nutr. Metab.
31
354-361
1987
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
2
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
395924
O'Fallon
Cellular localization of alpha ...
Oryctolagus cuniculus, Rattus norvegicus
Biochim. Biophys. Acta
499
321-328
1977
-
-
-
-
-
-
-
-
2
-
-
2
-
5
-
-
-
-
-
4
-
-
4
-
2
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
4
-
-
4
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
395925
Zaror-Behrens
-
Further studies on 2-oxoglutar ...
Tetrahymena pyriformis
Int. J. Biochem.
7
111-116
1976
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
395926
Zaror-Behrens
Demonstration of 2-oxoglutarat ...
Tetrahymena pyriformis
Can. J. Biochem.
51
1505-1508
1973
-
-
-
-
-
-
1
-
1
2
-
-
-
2
-
-
-
-
-
-
2
-
2
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
2
-
-
-
-
-
-
-
-
2
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
349030
Hirabayashi
-
Inhibition of the alpha-ketogl ...
Saccharomyces cerevisiae
Agric. Biol. Chem.
36
1249-1251
1972
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
395935
Prather
-
Glycine and glyoxylate decarbo ...
Nicotiana rustica
Phytochemistry
11
1637-1647
1972
-
-
-
-
-
-
-
-
1
2
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
2
-
1
-
-
-
-
-
1
-
-
2
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
395927
Yamasaki
Purification, general properti ...
Mycolicibacterium phlei
Biochim. Biophys. Acta
242
637-647
1971
-
-
-
-
-
-
4
2
-
2
-
-
-
2
-
-
1
1
-
-
1
-
3
-
1
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
4
-
2
-
2
-
-
-
-
-
1
-
-
1
-
3
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
395928
Schlossberg
Carboligase activity of alpha- ...
Bos taurus, Rattus norvegicus
Biochemistry
9
1148-1153
1970
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
9
1
-
4
-
2
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
9
1
-
4
-
2
-
-
-
1
-
-
-
-
-
-
-
-
-
395932
Yamasaki
alpha-Ketoglutarate:glyoxylate ...
Escherichia coli
Biochem. Biophys. Res. Commun.
39
790-795
1970
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
395929
Schlossberg
Isolation and identification o ...
Bos taurus
Biochemistry
7
333-337
1968
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
395930
Steward
The synergistic decarboxylatio ...
Sus scrofa
Biochem. J.
102
885-897
1967
3
-
-
-
-
3
2
2
1
1
-
-
-
1
1
-
1
1
-
1
1
1
1
-
-
-
-
-
1
1
-
1
-
-
-
3
-
-
1
-
-
3
-
2
-
2
1
1
-
-
-
1
-
1
-
1
1
1
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-