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Literature summary for 2.1.1.86 extracted from

  • Wagner, T.; Ermler, U.; Shima, S.
    MtrA of the sodium ion pumping methyltransferase binds cobalamin in a unique mode (2016), Sci. Rep., 6, 28226 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.86

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Methanocaldococcus jannaschii
expressed in Escherichia coli BL21(DE3) cells Methanothermus fervidus

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with either hydroxocobalamin or methylcobalamin, vapor diffusion method, using 26% (w/v) PEG 3000, 100 mM Na citrate pH 5.5 Methanothermus fervidus
in complex with either hydroxocobalamin or methylcobalamin, vapor diffusion method, using 26% (w/v) PEG 3000, 2.2 M DL-malate pH 7.6, 100 mM Tris-HCl pH 8.0 Methanocaldococcus jannaschii

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Methanothermus fervidus 5737
-
membrane
-
 Methanocaldococcus jannaschii 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-methyl-5,6,7,8-tetrahydromethanopterin + CoM + 2 Na+/in Methanocaldococcus jannaschii
-
 5,6,7,8-tetrahydromethanopterin + 2-(methylsulfanyl)ethane-1-sulfonate + 2 Na+/out
-
 ?
5-methyl-5,6,7,8-tetrahydromethanopterin + CoM + 2 Na+/in Methanothermus fervidus
-
 5,6,7,8-tetrahydromethanopterin + 2-(methylsulfanyl)ethane-1-sulfonate + 2 Na+/out
-
 ?
5-methyl-5,6,7,8-tetrahydromethanopterin + CoM + 2 Na+/in Methanothermus fervidus ATCC 43054
-
 5,6,7,8-tetrahydromethanopterin + 2-(methylsulfanyl)ethane-1-sulfonate + 2 Na+/out
-
 ?

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii
-
-
-
Methanothermus fervidus E3GWY7
-
-
Methanothermus fervidus ATCC 43054 E3GWY7
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+ -charged HiTrap column chromatography Methanocaldococcus jannaschii
Ni2+ -charged HiTrap column chromatography Methanothermus fervidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-methyl-5,6,7,8-tetrahydromethanopterin + CoM + 2 Na+/in
-
 Methanocaldococcus jannaschii 5,6,7,8-tetrahydromethanopterin + 2-(methylsulfanyl)ethane-1-sulfonate + 2 Na+/out
-
 ?
5-methyl-5,6,7,8-tetrahydromethanopterin + CoM + 2 Na+/in
-
 Methanothermus fervidus 5,6,7,8-tetrahydromethanopterin + 2-(methylsulfanyl)ethane-1-sulfonate + 2 Na+/out
-
 ?
5-methyl-5,6,7,8-tetrahydromethanopterin + CoM + 2 Na+/in
-
 Methanothermus fervidus ATCC 43054 5,6,7,8-tetrahydromethanopterin + 2-(methylsulfanyl)ethane-1-sulfonate + 2 Na+/out
-
 ?

Synonyms

Synonyms Comment Organism
CH3-H4MPT: CoM-SH methyltransferase complex
-
 Methanocaldococcus jannaschii
CH3-H4MPT: CoM-SH methyltransferase complex
-
 Methanothermus fervidus
methyl-tetrahydromethanopterin:coenzyme M methyltransferase complex
-
 Methanocaldococcus jannaschii
methyl-tetrahydromethanopterin:coenzyme M methyltransferase complex
-
 Methanothermus fervidus
MtrA-H complex
-
 Methanocaldococcus jannaschii
MtrA-H complex
-
 Methanothermus fervidus

Cofactor

Cofactor Comment Organism Structure
Cobalamin
-
 Methanocaldococcus jannaschii
Cobalamin
-
 Methanothermus fervidus