Application | Comment | Organism |
---|---|---|
additional information | protein model of PMT based on the crystal structure of SPDS suggests that overall protein folds are comparable, the respective cosubstrates S-adenosylmethionine and decarboxylated S-adenosylmethionine, however, appear to bind differentially to the active sites of both enzymes, and the substrate putrescine adopts a different position | Datura stramonium |
Cloned (Comment) | Organism |
---|---|
expressed in Rosetta-gami (DE3) cells | Solanum lycopersicum |
expressed in Rosetta-gami (DE3) cells | Calystegia sepium |
expressed in Rosetta-gami (DE3) cells | Physalis divaricata |
expressed in Rosetta-gami (DE3) cells | Solanum dulcamara |
expressed in Rosetta-gami (DE3) cells | Datura inoxia |
expressed in Rosetta-gami (DE3) cells | Datura stramonium |
into vector pET21d and transferred into Escherichia coli Rosetta-gami (DE3) cells | Physalis divaricata |
into vector pET21d and transferred into Escherichia coli Rosetta-gami (DE3) cells | Calystegia sepium |
into vector pET21d and transferred into Escherichia coli Rosetta-gami (DE3) cells | Solanum dulcamara |
into vector pET21d and transferred into Escherichia coli Rosetta-gami (DE3) cells | Datura inoxia |
into vector pET21d and transferred into Escherichia coli Rosetta-gami (DE3) cells | Datura stramonium |
into vector pET21d and transferred into Escherichia coli Rosetta-gami (DE3) cells | Solanum lycopersicum |
Protein Variants | Comment | Organism |
---|---|---|
I136V | reduced PMT activity, about half of the wild-type activity | Datura stramonium |
I141D | no PMT activity | Datura stramonium |
I141D | mutant shows no activity | Datura stramonium |
T117Q | no PMT activity | Datura stramonium |
T117Q | mutant shows no activity | Datura stramonium |
T117Q/I141D | no PMT activity | Datura stramonium |
T117Q/I141D | mutant shows no activity | Datura stramonium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.113 | - |
putrescine | - |
Solanum lycopersicum | |
0.12 | - |
putrescine | PMT1 | Calystegia sepium | |
0.12 | - |
putrescine | isoenzyme PMT1 | Calystegia sepium | |
0.169 | - |
putrescine | - |
Solanum dulcamara | |
0.281 | - |
putrescine | - |
Datura stramonium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Calystegia sepium | - |
- |
- |
Calystegia sepium | Q2KTH3 | - |
- |
Datura inoxia | Q2KTH1 | - |
- |
Datura inoxia | Q2KTH2 | - |
- |
Datura stramonium | Q70EW6 | - |
- |
Physalis divaricata | Q2KTH0 | - |
- |
Solanum dulcamara | - |
- |
- |
Solanum lycopersicum | - |
- |
- |
Solanum lycopersicum | Q2KTH4 | - |
- |
Purification (Comment) | Organism |
---|---|
gel filtration, approximately 95% pure | Physalis divaricata |
gel filtration, approximately 95% pure | Calystegia sepium |
gel filtration, approximately 95% pure | Solanum dulcamara |
gel filtration, approximately 95% pure | Datura inoxia |
gel filtration, approximately 95% pure | Datura stramonium |
gel filtration, approximately 95% pure | Solanum lycopersicum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Solanum lycopersicum | - |
root | - |
Calystegia sepium | - |
root | - |
Physalis divaricata | - |
root | - |
Solanum dulcamara | - |
root | - |
Datura inoxia | - |
root | - |
Datura stramonium | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + putrescine | - |
Solanum lycopersicum | S-adenosyl-L-homocysteine + N-methylputrescine | - |
? | |
S-adenosyl-L-methionine + putrescine | - |
Calystegia sepium | S-adenosyl-L-homocysteine + N-methylputrescine | - |
? | |
S-adenosyl-L-methionine + putrescine | - |
Physalis divaricata | S-adenosyl-L-homocysteine + N-methylputrescine | - |
? | |
S-adenosyl-L-methionine + putrescine | - |
Solanum dulcamara | S-adenosyl-L-homocysteine + N-methylputrescine | - |
? | |
S-adenosyl-L-methionine + putrescine | - |
Datura inoxia | S-adenosyl-L-homocysteine + N-methylputrescine | - |
? | |
S-adenosyl-L-methionine + putrescine | - |
Datura stramonium | S-adenosyl-L-homocysteine + N-methylputrescine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PMT | - |
Solanum lycopersicum |
PMT | - |
Physalis divaricata |
PMT | - |
Solanum dulcamara |
PMT | - |
Datura stramonium |
PMT1 | - |
Calystegia sepium |
PMT1 | - |
Datura inoxia |
PMT2 | - |
Calystegia sepium |
PMT2 | - |
Datura inoxia |
putrescine N-methyltransferase | - |
Solanum lycopersicum |
putrescine N-methyltransferase | - |
Calystegia sepium |
putrescine N-methyltransferase | - |
Physalis divaricata |
putrescine N-methyltransferase | - |
Solanum dulcamara |
putrescine N-methyltransferase | - |
Datura inoxia |
putrescine N-methyltransferase | - |
Datura stramonium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Solanum lycopersicum |
30 | - |
assay at | Calystegia sepium |
30 | - |
assay at | Physalis divaricata |
30 | - |
assay at | Solanum dulcamara |
30 | - |
assay at | Datura inoxia |
30 | - |
assay at | Datura stramonium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.047 | - |
putrescine | - |
Solanum dulcamara | |
0.086 | - |
putrescine | PMT2 | Calystegia sepium | |
0.086 | - |
putrescine | isoenzyme PMT2 | Calystegia sepium | |
0.16 | - |
putrescine | PMT1 | Calystegia sepium | |
0.16 | - |
putrescine | isoenzyme PMT1 | Calystegia sepium | |
0.19 | - |
putrescine | - |
Solanum lycopersicum | |
0.198 | - |
putrescine | - |
Physalis divaricata | |
0.359 | - |
putrescine | - |
Datura inoxia | |
0.383 | - |
putrescine | - |
Datura inoxia | |
0.383 | - |
putrescine | PMT1 | Datura inoxia | |
0.388 | - |
putrescine | - |
Datura stramonium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Physalis divaricata |
9 | - |
- |
Calystegia sepium |
9 | - |
- |
Solanum dulcamara |
9 | - |
- |
Datura inoxia |
9 | - |
- |
Datura stramonium |
9 | - |
- |
Solanum lycopersicum |
9 | - |
assay at | Solanum lycopersicum |
9 | - |
assay at | Calystegia sepium |
9 | - |
assay at | Physalis divaricata |
9 | - |
assay at | Solanum dulcamara |
9 | - |
assay at | Datura inoxia |
9 | - |
assay at | Datura stramonium |