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S-adenosyl-L-methionine + 1,4-diamino-2,3-dibromo-trans-but-2-ene
S-adenosyl-L-homocysteine + ?
-
-
-
-
?
S-adenosyl-L-methionine + 1,4-diamino-2-hydroxybutane
S-adenosyl-L-homocysteine + ?
-
-
-
-
?
S-adenosyl-L-methionine + 1,4-diamino-2-oxobutane
S-adenosyl-L-homocysteine + ?
-
-
-
-
?
S-adenosyl-L-methionine + 1,4-diamino-trans-but-2-ene
S-adenosyl-L-homocysteine + ?
-
-
-
-
?
S-adenosyl-L-methionine + 4-(aminomethyl)piperidine
S-adenosyl-L-homocysteine + ?
-
-
-
-
?
S-adenosyl-L-methionine + N-methylputrescine
S-adenosylhomocysteine + N,N-dimethylputrescine
-
about 8% of activity compared to putrescine
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
S-adenosyl-L-methionine + putrescine
S-adenosylhomocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + trans-1,4-diamonocyclohexane
S-adenosyl-L-homocysteine + ?
-
-
-
-
?
additional information
?
-
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme is probably involved in nicotine biosynthesis
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
enzyme is probably involved in nicotine biosynthesis
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
best substrate
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme is probably involved in nicotine biosynthesis
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
enzyme is probably involved in nicotine biosynthesis
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
the enzyme has high affinity with putrescine but low catalytic activity, suggesting that it is a rate-limiting enzyme
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme is probably involved in nicotine biosynthesis
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
enzyme is probably involved in nicotine biosynthesis
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
enzyme is probably involved in nicotine biosynthesis
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme possibly may play an additional, subtle role in alkaloid metabolsim in leaf tissue
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme is probably involved in nicotine biosynthesis
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme is probably involved in nicotine biosynthesis
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme is probably involved in nicotine biosynthesis
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
?
additional information
?
-
-
requirement for substrate activity: two amino groups in a trans conformation, separated by four carbon atoms
-
-
?
additional information
?
-
-
substrate with little activity: 1,4 -diamino-cis-but-2-ene, not: aliphatic monoamines, 1,3-diaminopropane, 1,5-diaminopentane, N,N`-dimethyl-1,4-diamino-trans-but-2-ene, 1,3-diamino-2-hydroxypropane, 1,3-diaminoacetone, 1,4-diaminobut-2-yne, N-carbamoylputrescine
-
-
?
additional information
?
-
inactive with n-butylamine, 1,6-diaminohexane and 1,7-diaminoheptane, the PMT enzyme of potato appears to be specific for putrescine, it does not accept other mono-, di- or tri-amines
-
-
?
additional information
?
-
-
inactive with n-butylamine, 1,6-diaminohexane and 1,7-diaminoheptane, the PMT enzyme of potato appears to be specific for putrescine, it does not accept other mono-, di- or tri-amines
-
-
?
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S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme is probably involved in nicotine biosynthesis
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
enzyme is probably involved in nicotine biosynthesis
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme is probably involved in nicotine biosynthesis
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
enzyme is probably involved in nicotine biosynthesis
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
the enzyme has high affinity with putrescine but low catalytic activity, suggesting that it is a rate-limiting enzyme
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme is probably involved in nicotine biosynthesis
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
enzyme is probably involved in nicotine biosynthesis
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
enzyme is probably involved in nicotine biosynthesis
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme possibly may play an additional, subtle role in alkaloid metabolsim in leaf tissue
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme is probably involved in nicotine biosynthesis
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme is probably involved in nicotine biosynthesis
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme catalyzes the initial reaction in the biosynthesis of tropane and pyrrolidine alkaloids from putrescine
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
enzyme is probably involved in nicotine biosynthesis
-
?
S-adenosyl-L-methionine + putrescine
S-adenosyl-L-homocysteine + N-methylputrescine
-
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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1,6-diaminohexane
weaker inhibitor
1,7-Diaminoheptane
weaker inhibitor
2-(aminomethyl)pyridine
-
-
4-(aminomethyl)pyridine
-
-
Ca2+
28% inhibition by 5 mM
Cr2+
31% inhibition by 5 mM
Cu2+
73% inhibition by 5 mM
EDTA
23% inhibition by 5 mM
Fe2+
33% inhibition by 5 mM
Fe3+
14% inhibition by 5 mM
Mg2+
28% inhibition by 5 mM
Mn2+
26% inhibition by 5 mM
N-methyl-1,3-diaminopropane
Ni2+
71% inhibition by 5 mM
p-chloromercuribenzoate
-
strong
S-adenosyl-L-homocysteine
Zn2+
27% inhibition by 5 mM
1,3-diaminopropane
-
Ag+
51% inhibition by 5 mM
cadaverine
-
cadaverine
strong inhibitor
n-butylamine
strong inhibitor; strong inhibitor
n-butylamine
strong inhibitor
n-butylamine
strong inhibitor; strong inhibitor
n-butylamine
strong inhibitor
n-butylamine
-
strong inhibitor
n-butylamine
strong inhibitor; strong inhibitor
n-butylamine
strong inhibitor
n-butylamine
strong inhibitor
n-butylamine
strong inhibitor
n-butylamine
-
strong inhibitor
n-butylamine
strong inhibitor; strong inhibitor
n-butylamine
strong inhibitor
n-butylamine
strong inhibitor; strong inhibitor
n-butylamine
strong inhibitor; strong inhibitor; strong inhibitor; strong inhibitor
n-butylamine
strong inhibitor
n-butylamine
strong inhibitor
n-butylamine
strong inhibitor
n-butylamine
strong inhibitor
n-butylamine
strong inhibitor
N-methyl-1,3-diaminopropane
-
N-methyl-1,3-diaminopropane
-
N-methyl-1,3-diaminopropane
-
N-methyl-1,3-diaminopropane
-
N-methyl-1,3-diaminopropane
-
-
N-methyl-1,3-diaminopropane
-
N-methyl-1,3-diaminopropane
-
N-methyl-1,3-diaminopropane
-
N-methyl-1,3-diaminopropane
-
N-methyl-1,3-diaminopropane
-
-
N-methyl-1,3-diaminopropane
-
N-methyl-1,3-diaminopropane
-
N-methyl-1,3-diaminopropane
-
N-methyl-1,3-diaminopropane
-
N-methyl-1,3-diaminopropane
-
N-methyl-1,3-diaminopropane
-
N-methyl-1,3-diaminopropane
-
N-methyl-1,3-diaminopropane
-
N-methyl-1,3-diaminopropane
-
N-methylputrescine
inhibitor with approximately competitive interaction kinetics to putrescine; inhibitor with approximately competitive interaction kinetics to putrescine
N-methylputrescine
inhibitor with approximately competitive interaction kinetics to putrescine
N-methylputrescine
inhibitor with approximately competitive interaction kinetics to putrescine; inhibitor with approximately competitive interaction kinetics to putrescine
N-methylputrescine
inhibitor with approximately competitive interaction kinetics to putrescine
N-methylputrescine
-
inhibitor with approximately competitive interaction kinetics to putrescine
N-methylputrescine
inhibitor with approximately competitive interaction kinetics to putrescine; inhibitor with approximately competitive interaction kinetics to putrescine
N-methylputrescine
inhibitor with approximately competitive interaction kinetics to putrescine
N-methylputrescine
inhibitor with approximately competitive interaction kinetics to putrescine
N-methylputrescine
inhibitor with approximately competitive interaction kinetics to putrescine
N-methylputrescine
-
inhibitor with approximately competitive interaction kinetics to putrescine
N-methylputrescine
inhibitor with approximately competitive interaction kinetics to putrescine; inhibitor with approximately competitive interaction kinetics to putrescine
N-methylputrescine
inhibitor with approximately competitive interaction kinetics to putrescine
N-methylputrescine
inhibitor with approximately competitive interaction kinetics to putrescine; inhibitor with approximately competitive interaction kinetics to putrescine
N-methylputrescine
inhibitor with approximately competitive interaction kinetics to putrescine; inhibitor with approximately competitive interaction kinetics to putrescine; inhibitor with approximately competitive interaction kinetics to putrescine; inhibitor with approximately competitive interaction kinetics to putrescine
N-methylputrescine
inhibitor with approximately competitive interaction kinetics to putrescine
N-methylputrescine
inhibitor with approximately competitive interaction kinetics to putrescine
N-methylputrescine
inhibitor with approximately competitive interaction kinetics to putrescine
N-methylputrescine
inhibitor with approximately competitive interaction kinetics to putrescine
N-methylputrescine
inhibitor with approximately competitive interaction kinetics to putrescine
S-adenosyl-L-homocysteine
-
competitive inhibitor
S-adenosyl-L-homocysteine
-
-
additional information
no enzyme activity without the addition of S-adenosyl-L-methionine
-
additional information
-
no enzyme activity without the addition of S-adenosyl-L-methionine
-
additional information
-
monoamines p.e. cyclohexylamine, exo-2-aminonorborane, n-butylamine, 4-methylcyclohexylamine, cyclopentylamine; not: 1,3-diaminopropane, 2-(2-aminoethyl)pyridine, 2-aminopyridine, 3-aminopyridine, 4-aminopyridine
-
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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AbPMT1 is expressed more strongly than ABPMT2 in the root
expressed in Arabidopsis thaliana roots or in the vascular tissues, including pericycles and endodermis, of the Hyoscyamus niger hairy root cultures
-
expressed in Escherichia coli
expressed in Escherichia coli M15[pREP4] cells
expressed in Escherichia coli Rosetta-gami (DE3), transformed with a pET-21d vector encoding pmt
-
expressed in Escherichia coli, three genes isolated from Nicotiana sylvestris: NsPMT1, NsPMT2, NsPMT3
expressed in roots and leaves
-
expressed in Rosetta-gami (DE3) cells
expressed in xylem-tissues of roots, three genes isolated from Nicotiana sylvestris: NsPMT1, NsPMT2, NsPMT3, a highly conserved NsPMT 5'-flanking region is sufficient for tissue-specific and jasmonate-responsive expression of all three PMT genes
into pETat-32a(+) vector and expressed in Escherichia coli BL21trxB(DE3)
into vector pET21d and transferred into Escherichia coli Rosetta-gami (DE3) cells
pmt1 and pmt2 cloned into pET-21d vector, PMT1 expressed in Escherichia coli Rosetta-gami (DE3), PMT2 expressed in Escherichia coli BL21 (DE3) and Rosetta-gami (DE3), pmt1 yields active enzymes, while pmt2 expression results in insoluble protein
T1 progeny of transgenic Atropa belladonna, in which putrescine N-methyltransferase (EC 2.1.1.53) from Nicotiana tabacum (NtPMT) and hyoscyamine 6beta-hydroxylase (EC 1.14.11.14) from Hyoscyamus niger (HnH6H) are overexpressed, are established to investigate tropane alkaloids biosynthesis and distribution in ripe fruits, leaves, stems, primary roots and secondary roots under field conditions. Both NtPMT and HnH6H are detected at the transcriptional level in transgenic plants, whereas they are not detected in wild-type plants. Metabolically engineering Atropa belladonna based on overexpressing NtPMT and HnH6H shows a chemotype of rich scopolamine that greatly improves the commercial and pharmaceutical properties of Atropa belladonna
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tobacco pmt gene with a selectable marker (nptII) gene and the T-DNA of pRiA4 of Agrobacterium rhizogenes introduced into Hyoscyamus niger leaf explants using disarmed Agrobacterium tumefaciens C58C1 strain
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AbPMT1 is expressed more strongly than ABPMT2 in the root
AbPMT1 is expressed more strongly than ABPMT2 in the root
expressed in Escherichia coli
-
expressed in Escherichia coli
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expressed in Escherichia coli
expressed in Escherichia coli
expressed in Escherichia coli
expressed in Escherichia coli
expressed in Escherichia coli
expressed in Escherichia coli
expressed in Escherichia coli
expressed in Escherichia coli
expressed in Escherichia coli
expressed in Escherichia coli
expressed in Escherichia coli
expressed in Escherichia coli
expressed in Escherichia coli
expressed in Escherichia coli
expressed in Escherichia coli
expressed in Escherichia coli
expressed in Escherichia coli
expressed in Rosetta-gami (DE3) cells
expressed in Rosetta-gami (DE3) cells
expressed in Rosetta-gami (DE3) cells
expressed in Rosetta-gami (DE3) cells
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expressed in Rosetta-gami (DE3) cells
expressed in Rosetta-gami (DE3) cells
into vector pET21d and transferred into Escherichia coli Rosetta-gami (DE3) cells
into vector pET21d and transferred into Escherichia coli Rosetta-gami (DE3) cells
into vector pET21d and transferred into Escherichia coli Rosetta-gami (DE3) cells
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into vector pET21d and transferred into Escherichia coli Rosetta-gami (DE3) cells
into vector pET21d and transferred into Escherichia coli Rosetta-gami (DE3) cells
into vector pET21d and transferred into Escherichia coli Rosetta-gami (DE3) cells
tobacco pmt gene with a selectable marker (nptII) gene and the T-DNA of pRiA4 of Agrobacterium rhizogenes introduced into Hyoscyamus niger leaf explants using disarmed Agrobacterium tumefaciens C58C1 strain
-
tobacco pmt gene with a selectable marker (nptII) gene and the T-DNA of pRiA4 of Agrobacterium rhizogenes introduced into Hyoscyamus niger leaf explants using disarmed Agrobacterium tumefaciens C58C1 strain
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2006
Hyoscyamus niger, Nicotiana tabacum
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brenda
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Datura stramonium
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Putrescine N-methyltransferase--the start for alkaloids
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Atropa belladonna
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Nicotiana tabacum
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Physalis divaricata
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Hyoscyamus niger
brenda