Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.1 extracted from

  • Loring, H.S.; Thompson, P.R.
    Kinetic mechanism of nicotinamide N-methyltransferase (2018), Biochemistry, 57, 5524-5532 .
    View publication on PubMed
Search for more literature for 2.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1-methylnicotinamide product inhibition Homo sapiens
S-adenosyl-L-homocysteine product inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 nicotinamide pH 8.6, 37°C Homo sapiens
0.01
-
 S-adenosyl-L-methionine pH 8.6, 37°C Homo sapiens
0.01
-
 Quinoline pH 8.6, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P40261
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information NNMT uses a rapid equilibrium ordered mechanism, where NNMT first binds S-adenosyl-L-methionine, which is followed by nicotinamide. Methyl transfer occurs, and methylated nicotinamide and S-adenosylhomocysteine are released consecutively Homo sapiens ?
-
-
S-adenosyl-L-methionine + nicotinamide
-
 Homo sapiens S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
 ?
S-adenosyl-L-methionine + quinoline
-
 Homo sapiens S-adenosyl-L-homocysteine + N-methylquinoline
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.008
-
 S-adenosyl-L-methionine pH 8.6, 37°C Homo sapiens
0.011
-
 Quinoline pH 8.6, 37°C Homo sapiens
0.014
-
 nicotinamide pH 8.6, 37°C Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0012
-
 substrate S-adenosyl-L-methionine, pH 8.6, 37°C Homo sapiens S-adenosyl-L-homocysteine
0.002
-
 substrate quinoline, pH 8.6, 37°C Homo sapiens S-adenosyl-L-homocysteine
0.023
-
 substrate quinoline, pH 8.6, 37°C Homo sapiens 1-methylnicotinamide

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.75
-
 S-adenosyl-L-methionine pH 8.6, 37°C Homo sapiens
1.1
-
 Quinoline pH 8.6, 37°C Homo sapiens
4.7
-
 nicotinamide pH 8.6, 37°C Homo sapiens