General Stability | Organism |
---|---|
glycerol stabilizes | Sus scrofa |
thiols required for stability during isolation | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
S-adenosyl-L-methionine | - |
Sus scrofa | |
0.2 | - |
1,2,3,4-tetrahydroisoquinoline | - |
Sus scrofa | |
0.83 | - |
nicotinamide | - |
Sus scrofa | |
1.3 | - |
Quinoline | - |
Sus scrofa | |
1.5 | - |
Isoquinoline | - |
Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
1 * 27000, SDS-PAGE | Sus scrofa |
27000 | 30000 | gel filtration, SDS-PAGE | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Sus scrofa | enzyme may function in detoxificating of numerous alkaloids | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
assay | Sus scrofa |
Storage Stability | Organism |
---|---|
-20°C, 20% glycerol w/v, stable for 1 month, partially purified enzyme | Sus scrofa |
4°C, 20% glycerol w/v, pH 7.6, 30% loss of activity, purified enzyme | Sus scrofa |
4°C, 20% w/v glycerol, stable for 1 week, partially purified enzyme | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme may function in detoxificating of numerous alkaloids | Sus scrofa | ? | - |
? | |
S-adenosyl-L-methionine + 1,2,3,4-tetrahydroisoquinoline | - |
Sus scrofa | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + 3-acetylpyridine | - |
Sus scrofa | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + isoquinoline | - |
Sus scrofa | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + nicotinamide | - |
Sus scrofa | S-adenosyl-L-homocysteine + 1-methylnicotinamide | - |
? | |
S-adenosyl-L-methionine + quinoline | - |
Sus scrofa | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + thionicotinamide | - |
Sus scrofa | S-adenosyl-L-homocysteine + 1-methylthionicotinamide | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 27000, SDS-PAGE | Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Sus scrofa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.7 | 9.8 | about 50% of maximum activity at pH 5.7 and 9.8 | Sus scrofa |