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Literature summary for 1.5.3.25 extracted from

  • Capuano, E.; Fedele, F.; Mennella, C.; Visciano, M.; Napolitano, A.; Lanzuise, S.; Ruocco, M.; Lorito, M.; del Castillo, M.D.; Fogliano, V.
    Studies on the effect of amadoriase from Aspergillus fumigatus on peptide and protein glycation in vitro (2007), J. Agric. Food Chem., 55, 4189-4195 .
    View publication on PubMed
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Application

Application Comment Organism
synthesis addition of amadoriase I during peptide glycation reaction reduces the formation of Amadori products up to 80%. The effect is more evident for hydrophobic peptides. In protein-glucose systems, the effect is dependent on the molecular weight and steric hindrance being negligible for BSA and at a maximum for insulin Aspergillus fumigatus

Cloned(Commentary)

Cloned (Comment) Organism
expression of isoform amadoriase I in Escherichai coli Aspergillus fumigatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5
-
 deoxy-D-fructosyl-AcPhe-Lys pH 7.4, 37°C Aspergillus fumigatus
1.7
-
 deoxy-D-fructosyl-AcGly-Lys pH 7.4, 37°C Aspergillus fumigatus
4.2
-
 Nepsilon-(1-deoxy-D-fructos-1-yl)-L-lysine pH 7.4, 37°C Aspergillus fumigatus
8.6
-
 deoxy-D-fructosyl-Lys-Phe pH 7.4, 37°C Aspergillus fumigatus
10.3
-
 deoxy-D-fructosyl-Lys-Ser pH 7.4, 37°C Aspergillus fumigatus

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxy-D-fructosyl-AcGly-Lys + O2 + H2O
-
 Aspergillus fumigatus ? + L-lysine + H2O2
-
 ?
deoxy-D-fructosyl-AcPhe-Lys + O2 + H2O
-
 Aspergillus fumigatus ? + L-lysine + H2O2
-
 ?
deoxy-D-fructosyl-Lys-Phe + O2 + H2O
-
 Aspergillus fumigatus ? + L-lysine + H2O2
-
 ?
deoxy-D-fructosyl-Lys-Ser + O2 + H2O
-
 Aspergillus fumigatus ? + L-lysine + H2O2
-
 ?
Nepsilon-(1-deoxy-D-fructos-1-yl)-L-lysine + O2 + H2O
-
 Aspergillus fumigatus ? + L-lysine + H2O2
-
 ?

Subunits

Subunits Comment Organism
? x * 53000, SDS-PAGE, recombinant isoform amadoriase I Aspergillus fumigatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.3
-
 deoxy-D-fructosyl-Lys-Ser pH 7.4, 37°C Aspergillus fumigatus
0.5
-
 deoxy-D-fructosyl-Lys-Phe pH 7.4, 37°C Aspergillus fumigatus
1.1
-
 Nepsilon-(1-deoxy-D-fructos-1-yl)-L-lysine pH 7.4, 37°C Aspergillus fumigatus
1.4
-
 deoxy-D-fructosyl-AcGly-Lys pH 7.4, 37°C Aspergillus fumigatus
1.8
-
 deoxy-D-fructosyl-AcPhe-Lys pH 7.4, 37°C Aspergillus fumigatus