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Literature summary for 1.5.3.25 extracted from

  • Fujiwara, M.; Sumitani, J.; Koga, S.; Yoshioka, I.; Kouzuma, T.; Imamura, S.; Kawaguchi, T.; Arai, M.
    Alteration of substrate specificity of fructosyl-amino acid oxidase from Fusarium oxysporum (2007), Appl. Microbiol. Biotechnol., 74, 813-819 .
No PubMed abstract available
Search for more literature for 1.5.3.25

Protein Variants

Protein Variants Comment Organism
K373M catalytic turnover for fructosyl valine greatly decreases, whereas that for Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine does not Fusarium oxysporum f. sp. raphani
K373R mutant has lost the activity on substrate fructosyl valine Fusarium oxysporum f. sp. raphani
K373T catalytic turnover for fructosyl valine greatly decreases, whereas that for Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine does not Fusarium oxysporum f. sp. raphani
K373V catalytic turnover for fructosyl valine greatly decreases, whereas that for Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine does not Fusarium oxysporum f. sp. raphani
K373W catalytic turnover for fructosyl valine greatly decreases, whereas that for Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine does not Fusarium oxysporum f. sp. raphani

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 20 mM, 14% residual activity Fusarium oxysporum f. sp. raphani
additional information no inhibiting: EDTA Fusarium oxysporum f. sp. raphani
Ni2+ 20 mM, 26% residual activity Fusarium oxysporum f. sp. raphani
Zn2+ 20 mM, 9% residual activity Fusarium oxysporum f. sp. raphani

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine wild-type, presence of 20 mM MgCl2, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
0.19
-
 Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine wild-type, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
0.22
-
 Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine mutant K373W, presence of 20 mM MgCl2, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
0.5
-
 Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine mutant K373V, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
0.88
-
 fructosyl L-valine wild-type, presence of 20 mM MgCl2, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
0.98
-
 fructosyl L-alanine wild-type, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
0.98
-
 Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine mutant K373T, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
1.19
-
 Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine mutant K373W, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
1.37
-
 fructosyl L-valine wild-type, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
1.51
-
 Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine mutant K373M, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
2.42
-
 Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine mutant K373R, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
6.55
-
 epsilon-fructosyl-aminocaproate wild-type, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
9.63
-
 gamma-fructosyl-aminobutanoate wild-type, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
14.3
-
 fructosyl L-valine mutant K373W, presence of 20 mM MgCl2, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
17.6
-
 fructosyl L-valine mutant K373V, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
18.4
-
 fructosyl L-valine mutant K373T, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
19
-
 fructosyl L-valine mutant K373M, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
20.8
-
 fructosyl L-valine mutant K373W, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
27.8
-
 epsilon-fructosyl-aminocaproate mutant K373W, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
29.4
-
 fructosyl L-alanine mutant K373W, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
29.9
-
 fructosyl L-valine mutant K373R, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
47
-
 gamma-fructosyl-aminobutanoate mutant K373W, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ slight activation Fusarium oxysporum f. sp. raphani
Mg2+ slight activation Fusarium oxysporum f. sp. raphani
Mn2+ slight activation Fusarium oxysporum f. sp. raphani

Organism

Organism UniProt Comment Textmining
Fusarium oxysporum f. sp. raphani
-
-
-
Fusarium oxysporum f. sp. raphani NBRC 9972
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
epsilon-fructosyl-aminocaproate + O2 + H2O
-
 Fusarium oxysporum f. sp. raphani glucosone + aminocaproate + H2O2
-
 ?
epsilon-fructosyl-aminocaproate + O2 + H2O
-
 Fusarium oxysporum f. sp. raphani NBRC 9972 glucosone + aminocaproate + H2O2
-
 ?
fructosyl L-alanine + O2 + H2O
-
 Fusarium oxysporum f. sp. raphani glucosone + L-alanine + H2O2
-
 ?
fructosyl L-alanine + O2 + H2O
-
 Fusarium oxysporum f. sp. raphani NBRC 9972 glucosone + L-alanine + H2O2
-
 ?
fructosyl L-valine + O2 + H2O
-
 Fusarium oxysporum f. sp. raphani glucosone + L-valine + H2O2
-
 ?
fructosyl L-valine + O2 + H2O
-
 Fusarium oxysporum f. sp. raphani NBRC 9972 glucosone + L-valine + H2O2
-
 ?
gamma-fructosyl-aminobutanoate + O2 + H2O
-
 Fusarium oxysporum f. sp. raphani glucosone + aminobutanoate + H2O2
-
 ?
gamma-fructosyl-aminobutanoate + O2 + H2O
-
 Fusarium oxysporum f. sp. raphani NBRC 9972 glucosone + aminobutanoate + H2O2
-
 ?
Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine + O2 + H2O
-
 Fusarium oxysporum f. sp. raphani glucosone + N-alpha-benzyloxycarbonyl-L-lysine + H2O2
-
 ?
Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine + O2 + H2O
-
 Fusarium oxysporum f. sp. raphani NBRC 9972 glucosone + N-alpha-benzyloxycarbonyl-L-lysine + H2O2
-
 ?
Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-valine + O2 + H2O
-
 Fusarium oxysporum f. sp. raphani glucosone + N-alpha-benzyloxycarbonyl-L-valine + H2O2
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.04
-
 fructosyl L-valine mutant K373W, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
0.15
-
 fructosyl L-valine mutant K373M, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
0.29
-
 fructosyl L-valine mutant K373T, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
0.3
-
 fructosyl L-valine mutant K373V, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
0.51
-
 fructosyl L-valine mutant K373R, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
0.7
-
 fructosyl L-alanine mutant K373W, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
1.3
-
 fructosyl L-valine mutant K373W, presence of 20 mM MgCl2, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
1.55
-
 gamma-fructosyl-aminobutanoate mutant K373W, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
2.91
-
 epsilon-fructosyl-aminocaproate mutant K373W, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
8.27
-
 Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine mutant K373W, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
9.73
-
 gamma-fructosyl-aminobutanoate wild-type, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
10.6
-
 epsilon-fructosyl-aminocaproate wild-type, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
10.6
-
 Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine mutant K373W, presence of 20 mM MgCl2, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
10.9
-
 fructosyl L-valine wild-type, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
14.1
-
 Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine mutant K373T, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
15.5
-
 Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine wild-type, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
16
-
 Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine wild-type, presence of 20 mM MgCl2, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
16.1
-
 fructosyl L-valine wild-type, presence of 20 mM MgCl2, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
16.2
-
 Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine mutant K373V, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
17.3
-
 Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine mutant K373M, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
18.1
-
 Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine mutant K373R, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
21
-
 fructosyl L-alanine wild-type, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.002
-
 fructosyl L-valine mutant K373W, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
0.008
-
 fructosyl L-valine mutant K373M, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
0.016
-
 fructosyl L-valine mutant K373T, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
0.017
-
 fructosyl L-valine mutant K373R, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
0.017
-
 fructosyl L-valine mutant K373V, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
0.03
-
 gamma-fructosyl-aminobutanoate mutant K373W, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
0.088
-
 fructosyl L-valine mutant K373W, presence of 20 mM MgCl2, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
0.11
-
 epsilon-fructosyl-aminocaproate mutant K373W, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
0.24
-
 fructosyl L-alanine mutant K373W, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
1.01
-
 gamma-fructosyl-aminobutanoate wild-type, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
1.62
-
 epsilon-fructosyl-aminocaproate wild-type, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
6.96
-
 Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine mutant K373W, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
7.45
-
 Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine mutant K373R, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
7.97
-
 fructosyl L-valine wild-type, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
11.4
-
 Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine mutant K373M, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
14.4
-
 Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine mutant K373T, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
18.2
-
 fructosyl L-valine wild-type, presence of 20 mM MgCl2, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
21.4
-
 fructosyl L-alanine wild-type, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
32.4
-
 Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine mutant K373V, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
47.4
-
 Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine mutant K373W, presence of 20 mM MgCl2, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
80
-
 Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine wild-type, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani
106
-
 Nepsilon-fructosyl-Nalpha-benzyloxycarbonyl-L-lysine wild-type, presence of 20 mM MgCl2, pH 8.0, temperature not specified in the publication Fusarium oxysporum f. sp. raphani