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Literature summary for 1.5.3.25 extracted from

  • Akazawa, S.; Karino, T.; Yoshida, N.; Katsuragi, T.; Tani, Y.
    Functional analysis of fructosyl-amino acid oxidases of Aspergillus oryzae (2004), Appl. Environ. Microbiol., 70, 5882-5890 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.5.3.25

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
 Nalpha-benzyloxycarbonyl-L-lysine pH 8.5, temperature not specified in the publication Aspergillus oryzae
0.51
-
 Nalpha-benzyloxycarbonyl-L-lysine isoform FAOD-Ao1, pH 8.5, temperature not specified in the publication Aspergillus oryzae
1.38
-
 fructosyl L-valine pH 8.5, temperature not specified in the publication Aspergillus oryzae

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome
-
 Aspergillus oryzae 5777
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
 gel filtration Aspergillus oryzae
98000
-
 gel filtration Aspergillus oryzae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae Q60G78
-
-
Aspergillus oryzae Q60G79
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fructosyl L-valine + O2 + H2O
-
 Aspergillus oryzae glucosone + L-valine + H2O2
-
 ?
additional information no substrates: fructosyl L-valine, L-Pipecolate, sarcosine, and L-proline Aspergillus oryzae ?
-
-
additional information no substrates: L-Pipecolate, sarcosine, and L-proline Aspergillus oryzae ?
-
-
S: Nepsilon-fructosyl Nalpha-benzyloxycarbonyl-L-lysine + O2 + H2O
-
 Aspergillus oryzae glucosone + Nalpha-benzyloxycarbonyl-L-lysine + H2O2
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 48000, SDS-PAGE Aspergillus oryzae
monomer 1 * 49000, SDS-PAGE Aspergillus oryzae

Synonyms

Synonyms Comment Organism
FAOAo1
-
 Aspergillus oryzae
FAOAo2
-
 Aspergillus oryzae
FAOD-Ao1
-
 Aspergillus oryzae
FAOD-Ao2
-
 Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
 isoform FAOD-Ao1 Aspergillus oryzae
8.2
-
 isoform FAOD-Ao2 Aspergillus oryzae

General Information

General Information Comment Organism
physiological function conidia of the wild-type strain do not germinate on the medium containing fructosyl L-valine and NaNO2 as the sole carbon and nitrogen sources, respectively Aspergillus oryzae
physiological function wild-type strain grows on the medium containing fructosyl L-valine as the sole carbon and nitrogen sources, a strain lacking isoform FaD-Ao2 does not. Cconidia of the wild-type strain do not germinate on the medium containing fructosyl L-valine and NaNO2 as the sole carbon and nitrogen sources, respectively Aspergillus oryzae