Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | 1 mM, 76% residual activity | Pyrobaculum calidifontis | |
isocitrate | 10 M, 64% of initial activity | Pyrobaculum calidifontis | |
KCl | 100 mM, 45% residual activity | Pyrobaculum calidifontis | |
NaCl | 100 mM, 59% residual activity | Pyrobaculum calidifontis | |
oxaloacetate | 10 mM, 63% residual activity | Pyrobaculum calidifontis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
NADPH | pH 9.0, 50°C | Pyrobaculum calidifontis | |
0.035 | - |
NADP+ | pH 9.5, 50°C | Pyrobaculum calidifontis | |
1.7 | - |
2-oxoglutarate | pH 9.0, 50°C | Pyrobaculum calidifontis | |
2.2 | - |
NH3 | pH 9.0, 50°C | Pyrobaculum calidifontis | |
3.4 | - |
L-glutamate | pH 9.5, 50°C | Pyrobaculum calidifontis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
270000 | - |
gel filtration | Pyrobaculum calidifontis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrobaculum calidifontis | A3MWK6 | - |
- |
Pyrobaculum calidifontis JCM 11548 | A3MWK6 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxobutanoate + NH3 + NADPH + H+ | 2.6% of the activity with 2-oxoglutarate | Pyrobaculum calidifontis | L-2-aminobutanoate + H2O + NADP+ | - |
r | |
2-oxobutanoate + NH3 + NADPH + H+ | 2.6% of the activity with 2-oxoglutarate | Pyrobaculum calidifontis JCM 11548 | L-2-aminobutanoate + H2O + NADP+ | - |
r | |
2-oxoglutarate + NH3 + NADPH + H+ | - |
Pyrobaculum calidifontis | L-glutamate + H2O + NADP+ | - |
r | |
2-oxoglutarate + NH3 + NADPH + H+ | - |
Pyrobaculum calidifontis JCM 11548 | L-glutamate + H2O + NADP+ | - |
r | |
2-oxovalerate + NH3 + NADPH + H+ | 4.2% of the activity with 2-oxoglutarate | Pyrobaculum calidifontis | L-valine + H2O + NADP+ | - |
r | |
2-oxovalerate + NH3 + NADPH + H+ | 4.2% of the activity with 2-oxoglutarate | Pyrobaculum calidifontis JCM 11548 | L-valine + H2O + NADP+ | - |
r | |
L-glutamate + H2O + NADP+ | - |
Pyrobaculum calidifontis | 2-oxoglutarate + NH3 + NADPH + H+ | - |
r | |
L-glutamate + H2O + NADP+ | - |
Pyrobaculum calidifontis JCM 11548 | 2-oxoglutarate + NH3 + NADPH + H+ | - |
r | |
L-norvaline + H2O + NADP+ | 3% of the activity with L-glutamate | Pyrobaculum calidifontis | 2-oxopentanoate + NH3 + NADPH + H+ | - |
r | |
L-norvaline + H2O + NADP+ | 3% of the activity with L-glutamate | Pyrobaculum calidifontis JCM 11548 | 2-oxopentanoate + NH3 + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 47700, calculated | Pyrobaculum calidifontis |
Synonyms | Comment | Organism |
---|---|---|
Pcal_1606 | - |
Pyrobaculum calidifontis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.9 | - |
NADP+ | pH 9.5, 50°C | Pyrobaculum calidifontis | |
13 | - |
L-glutamate | pH 9.5, 50°C | Pyrobaculum calidifontis | |
92 | - |
NADPH | pH 9.0, 50°C | Pyrobaculum calidifontis | |
95 | - |
2-oxoglutarate | pH 9.0, 50°C | Pyrobaculum calidifontis | |
100 | - |
NH3 | pH 9.0, 50°C | Pyrobaculum calidifontis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | about 17% of the activity with NADP+ | Pyrobaculum calidifontis | |
NADH | about 7% of the activity with NADPH | Pyrobaculum calidifontis | |
NADP+ | - |
Pyrobaculum calidifontis | |
NADPH | - |
Pyrobaculum calidifontis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.8 | - |
L-glutamate | pH 9.5, 50°C | Pyrobaculum calidifontis | |
45 | - |
NH3 | pH 9.0, 50°C | Pyrobaculum calidifontis | |
56 | - |
2-oxoglutarate | pH 9.0, 50°C | Pyrobaculum calidifontis | |
280 | - |
NADP+ | pH 9.5, 50°C | Pyrobaculum calidifontis | |
5400 | - |
NADPH | pH 9.0, 50°C | Pyrobaculum calidifontis |