Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | at 1 mM, 86% inhibition | Bacillus sp. (in: Bacteria) | |
Ag2+ | at 1 mM, 60% inhibition | Bacillus sp. (in: Bacteria) | |
Hg2+ | at 1 mM, 70% inhibition | Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | - |
NADP+ | oxidative deamination at 30°C | Bacillus sp. (in: Bacteria) | |
0.044 | - |
NADPH | reductive amination at 30°C | Bacillus sp. (in: Bacteria) | |
3.13 | - |
2-oxoglutarate | reductive amination at 30°C | Bacillus sp. (in: Bacteria) | |
5.96 | - |
NH3 | reductive amination at 30°C | Bacillus sp. (in: Bacteria) | |
6.06 | - |
L-glutamate | oxidative deamination at 30°C | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
alpha6, 6 * 52000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
315000 | - |
gel filtration | Bacillus sp. (in: Bacteria) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + NADP+ + H2O | Bacillus sp. (in: Bacteria) | - |
2-oxoglutarate + NADPH + NH3 | - |
r | |
L-glutamate + NADP+ + H2O | Bacillus sp. (in: Bacteria) KSM-635 | - |
2-oxoglutarate + NADPH + NH3 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
- |
- |
Bacillus sp. (in: Bacteria) KSM-635 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus sp. (in: Bacteria) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
416 | - |
reductive amination at 30°C | Bacillus sp. (in: Bacteria) |
1140 | - |
reductive amination at pH 7.5 and 60°C | Bacillus sp. (in: Bacteria) |
Storage Stability | Organism |
---|---|
-20°C, phosphate buffer at pH 7.0, 0.2 mM 2-mercaptoethanol, 20 h, complete activity loss | Bacillus sp. (in: Bacteria) |
5°C, phosphate buffer at pH 7.0, 2 mM 2-mercaptoethanol, 6 months, no activity loss | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NH3 + NADH + H+ | NH4Cl used in enzyme assay | Bacillus sp. (in: Bacteria) | L-glutamate + H2O + NAD+ | - |
r | |
2-oxoglutarate + NH3 + NADH + H+ | NH4Cl used in enzyme assay | Bacillus sp. (in: Bacteria) KSM-635 | L-glutamate + H2O + NAD+ | - |
r | |
L-glutamate + NADP+ + H2O | - |
Bacillus sp. (in: Bacteria) | 2-oxoglutarate + NADPH + NH3 | - |
r | |
L-glutamate + NADP+ + H2O | - |
Bacillus sp. (in: Bacteria) KSM-635 | 2-oxoglutarate + NADPH + NH3 | - |
r |
Subunits | Comment | Organism |
---|---|---|
homohexamer | alpha6, 6 * 52000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
reductive amination and oxidative deamination | Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
irreversible inactivation | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
reductive amination | Bacillus sp. (in: Bacteria) |
8.5 | - |
oxidative deamination | Bacillus sp. (in: Bacteria) |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Bacillus sp. (in: Bacteria) | |
NADPH | - |
Bacillus sp. (in: Bacteria) |