Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, functional expression of wild-type enzyme in Escherichia coli strain BL21(DE3), expression of a mutant cloning artifact that has a seven residue deletion at the C-terminus and bores an additional 33 vector-derived residues | Mentha x piperita |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
(-)-isopiperitenone | pH 5.5, recombinant enzyme | Mentha x piperita | |
0.0022 | - |
NADPH | pH 5.5, recombinant enzyme | Mentha x piperita |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34409 | - |
2 * 34409, sequence calculation, 2 * 38000, SDS-PAGE | Mentha x piperita |
38000 | - |
2 * 34409, sequence calculation, 2 * 38000, SDS-PAGE | Mentha x piperita |
60000 | - |
about, gel filtration | Mentha x piperita |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-isopiperitenone + NADPH + H+ | Mentha x piperita | pathways of monoterpene biosynthesis, overview | (+)-cis-isopulegone + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mentha x piperita | - |
cv. Black Mitcham | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type enzyme from Escherichia coli strain BL21(DE3) to over 60% homogeneity, and to over 90% purity for the truncated mutant enzyme | Mentha x piperita |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epidermis | - |
Mentha x piperita | - |
leaf | - |
Mentha x piperita | - |
plant oil gland | - |
Mentha x piperita | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-isopiperitenone + NADPH + H+ | pathways of monoterpene biosynthesis, overview | Mentha x piperita | (+)-cis-isopulegone + NADP+ | - |
? | |
(-)-isopiperitenone + NADPH + H+ | regiospecific and stereospecific reductase | Mentha x piperita | (+)-cis-isopulegone + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 34409, sequence calculation, 2 * 38000, SDS-PAGE | Mentha x piperita |
Synonyms | Comment | Organism |
---|---|---|
More | (-)-isopiperitenone reductase is a member of the short-chain dehydrogenase/reductase superfamily | Mentha x piperita |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
NADPH | pH 5.5, recombinant enzyme | Mentha x piperita | |
1.3 | - |
(-)-isopiperitenone | pH 5.5, recombinant enzyme | Mentha x piperita |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
wild-type enzyme | Mentha x piperita |
6.5 | - |
truncated mutant enzyme | Mentha x piperita |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 7.5 | 50% of maximal activity within this range, wild-type enzyme | Mentha x piperita |
5 | 8 | 50% of maximal activity within this range, deletion mutant enzyme | Mentha x piperita |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | dependent on | Mentha x piperita |