KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
NADPH | pH 6.5, 30°C | Zea mays | |
0.058 | - |
NADH | pH 6.5, 30°C | Zea mays | |
0.06 | - |
D-erythro-sphinganine | pH 6.5, 30°C | Zea mays |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | associated with endoplasmic reticulum | Zea mays | 5783 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
shoot | - |
Zea mays | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-erythro-eicosasphinganine + NADH + H+ + O2 | - |
Zea mays | 4-hydroxyeicosasphinganine + NAD+ + H2O | - |
? | |
D-erythro-sphinganine + NADH + H+ + O2 | - |
Zea mays | 4-hydroxysphinganine + NAD+ + H2O | free 4-hydroxysphinganine is produced in excised corn shoots by the direct hydroxylation of sphinganine and not from the breakdown of complex sphingolipids | ? | |
D-erythro-sphinganine + NADPH + H+ + O2 | - |
Zea mays | 4-hydroxysphinganine + NADP+ + H2O | - |
? | |
additional information | DL-threo-sphinganine is not an effective substrate. Individual ceramide species having 2-, 6-,8-, or 24-carbon fatty acyl chains amide-linked to sphinganine are hydroxylated with similar efficiency as free sphinganine | Zea mays | ? | - |
? | |
N-acetylsphinganine + NADPH + H+ + O2 | - |
Zea mays | N-acetyl-4-hydroxysphinganine + NADP+ + H2O | - |
? | |
N-hexanoylsphinganine + NADPH + H+ + O2 | - |
Zea mays | N-hexanoyl-4-hydroxysphinganine + NADP+ + H2O | - |
? | |
N-octanoylsphinganine + NADPH + H+ + O2 | - |
Zea mays | N-octanoyl-4-hydroxysphinganine + NADP+ + H2O | - |
? |
Organism | Comment | Expression |
---|---|---|
Zea mays | sphinganine hydroxylase activity in microsomes isolated from corn shoots treated with fumonisin B1 increases more than 3fold compared to controls | up |