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Literature summary for 1.14.15.38 extracted from

  • Gu, T.; Zhou, C.; Sorensen, S.R.; Zhang, J.; He, J.; Yu, P.; Yan, X.; Li, S.
    The novel bacterial N-demethylase PdmAB is responsible for the initial step of N,N-dimethyl-substituted phenylurea herbicide degradation (2013), Appl. Environ. Microbiol., 79, 7846-7856 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.15.38

Application

Application Comment Organism
synthesis expression of PdmAB in Escherichia coli, Pseudomonas putida, and other sphingomonads results in a functional enzyme. Coexpression of a putative [3Fe-4S]-type ferredoxin from Sphingomonas sp. strain RW1 greatly enhances the catalytic activity of PdmAB in Escherichia coli Sphingobium sp. YBL2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Sphingobium sp. YBL2

Organism

Organism UniProt Comment Textmining
Sphingobium sp. YBL2 S5RR11 and S5SC42 S5RR11 i.e. subunit PudmA, S5SC42 i.e. subunit PudmB
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chlorotoluron + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 i.e. herbicide N'-(3-chloro-4-methylphenyl)-N,N-dimethylurea Sphingobium sp. YBL2 N-(3-chloro-4-methylphenyl)-N'-methylurea + formaldehyde + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
diuron + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
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 Sphingobium sp. YBL2 N-(3,4-dichlorophenyl)-N'-methylurea + formaldehyde + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
fenuron + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 i.e. herbicide N,N-dimethyl-N'-phenylurea Sphingobium sp. YBL2 N-methyl-N'-phenylurea + formaldehyde + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
fluometuron + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 i.e. herbicide N,N-dimethyl-N'-[3-(trifluoromethyl)phenyl]urea Sphingobium sp. YBL2 N-methyl-N'-[3-(trifluoromethyl)phenyl]urea + formaldehyde + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
isoproturon + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 i.e. herbicide N,N-dimethyl-N'-[4-(propan-2-yl)phenyl]urea Sphingobium sp. YBL2 N-methyl-N'-[4-(propan-2-yl)phenyl]urea + formaldehyde + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
metoxuron + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 i.e. herbicide N'-(3-chloro-4-methoxyphenyl)-N,N-dimethylurea Sphingobium sp. YBL2 N'-(3-chloro-4-methoxyphenyl)-N-methylurea + formaldehyde + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
monuron + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 i.e. herbicide N'-(4-chlorophenyl)-N,N-dimethylurea Sphingobium sp. YBL2 N-(4-chlorophenyl)-N'-methylurea + formaldehyde + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
additional information Escherichia coli cell suspensions expressing PudmAB can convert all the N,N-dimethyl-substituted phenylurea herbicides tested to their mono-N-demethylated derivatives but show no detectable activity for N-methoxy-N-methyl-substituted ones Sphingobium sp. YBL2 ?
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Synonyms

Synonyms Comment Organism
PudmA
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 Sphingobium sp. YBL2
PudmB
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 Sphingobium sp. YBL2

Cofactor

Cofactor Comment Organism Structure
additional information PdmAB has a low specificity for electron transport components and its optimal ferredoxin may be the [3Fe-4S] type Sphingobium sp. YBL2

General Information

General Information Comment Organism
physiological function the pdmAB genes are responsible for the initial mono-N-demethylation of N,N-dimethyl-substituted phenylurea herbicides. PdmAB is the oxygenase component of a bacterial Rieske non-heme iron oxygenase system. A subunit PudmA disruption strain does not show the ability to degrade isoproturon and other N,N-dimethyl substituted phenylurea herbicides, but it can still degrade the known metabolites of isoproturon Sphingobium sp. YBL2