BRENDA - Enzyme Database
show all sequences of 1.14.14.56

Metabolism of 1,8-cineole by human cytochrome P450 enzymes: identification of a new hydroxylated metabolite

Duisken, M.; Sandner, F.; Bloemeke, B.; Hollender, J.; Biochim. Biophys. Acta 1722, 304-311 (2005)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.012
-
1,8-cineole
pH 7.4, 37°C, formation of 2-hydroxy-1,8-cineole by isoform CYP3A4
Homo sapiens
0.019
-
1,8-cineole
pH 7.4, 37°C, formation of 3-hydroxy-1,8-cineole by isoform CYP3A4
Homo sapiens
0.141
-
1,8-cineole
pH 7.4, 37°C, formation of 3-hydroxy-1,8-cineole by isoform CYP3A5
Homo sapiens
0.599
-
1,8-cineole
pH 7.4, 37°C, formation of 2-hydroxy-1,8-cineole by isoform CYP3A5
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Homo sapiens
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,8-cineole + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
717270
Homo sapiens
2alpha-hydroxy-1,8-cineole + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1,8-cineole + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
717270
Homo sapiens
3alpha-hydroxy-1,8-cineole + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.012
-
1,8-cineole
pH 7.4, 37°C, formation of 2-hydroxy-1,8-cineole by isoform CYP3A4
Homo sapiens
0.019
-
1,8-cineole
pH 7.4, 37°C, formation of 3-hydroxy-1,8-cineole by isoform CYP3A4
Homo sapiens
0.141
-
1,8-cineole
pH 7.4, 37°C, formation of 3-hydroxy-1,8-cineole by isoform CYP3A5
Homo sapiens
0.599
-
1,8-cineole
pH 7.4, 37°C, formation of 2-hydroxy-1,8-cineole by isoform CYP3A5
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Homo sapiens
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,8-cineole + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
717270
Homo sapiens
2alpha-hydroxy-1,8-cineole + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1,8-cineole + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
717270
Homo sapiens
3alpha-hydroxy-1,8-cineole + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.056
-
1,8-cineole
pH 7.4, 37°C, formation of 3-hydroxy-1,8-cineole by isoform CYP3A4
Homo sapiens
0.08
-
1,8-cineole
pH 7.4, 37°C, formation of 2-hydroxy-1,8-cineole by isoform CYP3A4
Homo sapiens
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.056
-
1,8-cineole
pH 7.4, 37°C, formation of 3-hydroxy-1,8-cineole by isoform CYP3A4
Homo sapiens
0.08
-
1,8-cineole
pH 7.4, 37°C, formation of 2-hydroxy-1,8-cineole by isoform CYP3A4
Homo sapiens
Other publictions for EC 1.14.14.56
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
717270
Duisken
Metabolism of 1,8-cineole by h ...
Homo sapiens
Biochim. Biophys. Acta
1722
304-311
2005
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1
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4
1
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1
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1
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1
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1
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2
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2
2
717531
Miyazawa
Oxidation of 1,8-cineole, the ...
Rattus norvegicus
Drug Metab. Dispos.
29
200-205
2001
2
-
1
-
-
-
1
2
1
-
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1
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1
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1
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2
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1
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1
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1
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1
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1
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1
1
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718453
Miyazawa
Roles of cytochrome P450 3A en ...
Homo sapiens, Rattus norvegicus
Xenobiotica
31
713-723
2001
2
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2
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2
3
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2
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