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Literature summary for 1.14.14.22 extracted from

  • Ohshiro, T.; Ishii, Y.; Matsubara, T.; Ueda, K.; Izumi, Y.; Kino, K.; Kirimura, K.
    Dibenzothiophene desulfurizing enzymes from moderately thermophilic bacterium Bacillus subtilis WU-S2B: purification, characterization and overexpression (2005), J. Biosci. Bioeng., 100, 266-273.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene bdsA, recombinant overexpression in Escherichia coli, coexpression with chaperone groEL/groES genes Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Bacillus subtilis
4-chloromercuribenzoate
-
Bacillus subtilis
Ag+
-
Bacillus subtilis
biphenyl
-
Bacillus subtilis
Co2+
-
Bacillus subtilis
Cu2+
-
Bacillus subtilis
EDTA
-
Bacillus subtilis
Hg2+
-
Bacillus subtilis
Ni2+
-
Bacillus subtilis
Zn2+
-
Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
4 * 45000, SDS-PAGE Bacillus subtilis
174000
-
recombinant enzyme, gel filtration Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 Bacillus subtilis
-
2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
gene bdsA
-
Bacillus subtilis WU-S2B
-
gene bdsA
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 82.6fold from strain WU-S2B by three different steps of anion exchange chromatography and one step of hydrophobic interaction chromatography, gel filtration, and another step of anion exchange chromatography, recombinant enzyme from Escherichia coli 17.0fold to homogeneity by anion exchange and hydrophobic interaction chromatography, and again anion exchange chromatography Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
855
-
purified native enzyme, pH 7.0, 50┬░C Bacillus subtilis
875
-
purified recombinant enzyme, pH 7.0, 50┬░C Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2
-
Bacillus subtilis 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O
-
?
additional information coupled reaction with flavin reductase (TdsD) from thermophilic Paenibacillus sp. strain A11-2 Bacillus subtilis ?
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 45000, SDS-PAGE Bacillus subtilis

Synonyms

Synonyms Comment Organism
BdsA
-
Bacillus subtilis
DBT sulfone monooxygenase
-
Bacillus subtilis

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
50
-
-
Bacillus subtilis

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
50
-
purified enzyme, 30 min, 30% activity remaning Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Bacillus subtilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10 purified enzyme, stable at Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
FMNH2
-
Bacillus subtilis