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Literature summary for 1.14.14.137 extracted from

  • Liu, S.; Lv, Y.; Wan, X.R.; Li, L.M.; Hu, B.; Li, L.
    Cloning and expression analysis of cDNAs encoding ABA 8'-hydroxylase in peanut plants in response to osmotic stress (2014), PLoS ONE, 9, e97025 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene CYP707A1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, real-time quantitative RT-PCR enzyme expression analysis. Functional expression of AhCYP707A1 cDNA in yeast with recombinant AhCYP707A1 ABA 8’-hydroxylase catalytic activity in the microsomal fractions Arachis hypogaea
gene CYP707A2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, real-time quantitative RT-PCR enzyme expression analysis Arachis hypogaea

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Arachis hypogaea
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(+)-abscisate + [reduced NADPH-hemoprotein reductase] + O2 Arachis hypogaea
-
8'-hydroxyabscisate + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Organism

Organism UniProt Comment Textmining
Arachis hypogaea U6NHS3 cv. Yueyou 7
-
Arachis hypogaea U6NJF1 cv. Yueyou 7
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Arachis hypogaea
-
additional information isozymes AhCYP707A1 and AhCYP707A2 are expressed ubiquitously in peanut roots, stems, and leaves with different transcript accumulation levels, including the higher expression of AhCYP707A1 in roots Arachis hypogaea
-
root
-
Arachis hypogaea
-
root high expression Arachis hypogaea
-
seedling
-
Arachis hypogaea
-
stem
-
Arachis hypogaea
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-abscisate + [reduced NADPH-hemoprotein reductase] + O2
-
Arachis hypogaea 8'-hydroxyabscisate + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Subunits

Subunits Comment Organism
? x * 53390, sequence calculation Arachis hypogaea
? x * 55060, sequence calculation Arachis hypogaea

Synonyms

Synonyms Comment Organism
ABA 8'-hydroxylase
-
Arachis hypogaea
AhCYP707A1
-
Arachis hypogaea
AhCYP707A2
-
Arachis hypogaea
CYP707A1
-
Arachis hypogaea
CYP707A2
-
Arachis hypogaea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at Arachis hypogaea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
assay at Arachis hypogaea

Cofactor

Cofactor Comment Organism Structure
cytochrome P-450
-
Arachis hypogaea
NADPH-hemoprotein reductase A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ Arachis hypogaea

pI Value

Organism Comment pI Value Maximum pI Value
Arachis hypogaea sequence calculation
-
9.16
Arachis hypogaea sequence calculation
-
9.22

Expression

Organism Comment Expression
Arachis hypogaea susceptibility to osmotic stress and the resistance to salt ions in peanut seedlings of CYP707A isozymes. The osmotic stress instead of ionic stress affects the expression of those genes and the biosynthesis of abscisic acid in peanut. No effect by Li+ additional information
Arachis hypogaea expression of AhCYP707A2 is significantly up-regulated by 20% PEG 6000 or 250 mmol/l NaCl in peanut roots, stems, and leaves, whereas the up-regulation of AhCYP707A1 transcript level by PEG 6000 or NaCl is observed only in roots instead of leaves and stems up

General Information

General Information Comment Organism
evolution the enzyme belongs to the cytochrome P450 CYP707A family. The expressions of isozymes AhCYP707A1 and AhCYP707A2 play an important role in abscisic acid catabolism in peanut, particularly in response to osmotic stress Arachis hypogaea
physiological function abscisic acid (ABA) catabolism is one of the determinants of endogenous ABA levels affecting numerous aspects of plant growth and abiotic-stress responses. The major ABA catabolic pathway is triggered by ABA 8’-hydroxylation catalysed by ABA 8'-hydroxylase Arachis hypogaea