Cloned (Comment) | Organism |
---|---|
gene TaCYP707A1B, cloning from seedlings, expression analysis, ectopic expression of genes TaNCED2A and TaCYP707A1B in Arabidopsis thaliana Col-0 ecotype, using Agrobacterium tumefaciens AGL1 transformation method, results in altered seed abscisic acid level and dormancy with no effect on leaf abscisic acid content and transpirational water loss, phenotypes, overview. Quantitative and semiquantitative real-time and RT-PCR expression analysis | Triticum aestivum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of Tricticum aestivum mutant lines cyp707a1, cyp707a2, and cyp707a1/cyp707a2, phenotypes, overview | Triticum aestivum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-abscisate + [reduced NADPH-hemoprotein reductase] + O2 | Triticum aestivum | - |
8'-hydroxyabscisate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Triticum aestivum | B8QBY2 | cv. AC Domain | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | spatiotemporal expression pattern of TaCYP707A1B gene in hexaploid wheat | Triticum aestivum | - |
silique | CYP707A1 transcripts are highly abundant in the silique | Triticum aestivum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-abscisate + [reduced NADPH-hemoprotein reductase] + O2 | - |
Triticum aestivum | 8'-hydroxyabscisate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ABA 8'-hydroxylase | - |
Triticum aestivum |
ABA8'OH | - |
Triticum aestivum |
CYP707A1 | - |
Triticum aestivum |
cytochrome P450 monooxygenase 707A1 | - |
Triticum aestivum |
TaABA8'OH1 | - |
Triticum aestivum |
TaCYP707A1B | - |
Triticum aestivum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P-450 | - |
Triticum aestivum | |
NADPH-hemoprotein reductase | A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ | Triticum aestivum |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a member of the CYP707A family | Triticum aestivum |
malfunction | deletion in TaABA8'OH1 leads to increased abscisic acid level in the spikes and therefore increased drought sensitivity, phenotype, overview. Germination of the cyp707a1 cyp707a2 seeds appear not to be affected by the cold treatment, which induces early germination phenotype in the control wild-type and TaCYP707A1B-C seeds of transgenic Arabidospis thaliana, similar rate of transpirational water loss between the wild-type and mutant TaNCED2A-OE, cyp707a1/cyp707a2 and TaCYP707A1B-C lines during the entire period of dehydration | Triticum aestivum |
metabolism | biosynthesis and catabolism of abscisic acid (ABA) in plants are primarily regulated by 9-cis-epoxycarotenoid dioxygenases (NCEDs) and ABA 8'-hydroxylase (ABA8'OH), respectively | Triticum aestivum |
physiological function | the abscisic acid catabolic enzyme ABA8'OH is encoded by members of the cytochrome P450 monooxygenase 707A (CYP707A) gene family, which play important roles in regulating seed abscisic acid level during seed development and therefore dormancy. Role of the B genome copy of the cytochrome P450 monooxygenase 707A1 (CYP707A1) gene of hexaploid wheat (TaCYP707A1B), which encodes ABA8'OH, in regulating seed dormancy and leaf dehydration tolerance | Triticum aestivum |