Cloned (Comment) | Organism |
---|---|
gene cyp707A1, quantitative RT-PCR expression analysis | Citrus sinensis x Citrus trifoliata |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-abscisate + [reduced NADPH-hemoprotein reductase] + O2 | Citrus sinensis x Citrus trifoliata | - |
8'-hydroxyabscisate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrus sinensis x Citrus trifoliata | - |
Poncirus trifoliata x Citrus sinensis | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Citrus sinensis x Citrus trifoliata | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-abscisate + [reduced NADPH-hemoprotein reductase] + O2 | - |
Citrus sinensis x Citrus trifoliata | 8'-hydroxyabscisate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ABA 8'-hydroxylase | - |
Citrus sinensis x Citrus trifoliata |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P-450 | - |
Citrus sinensis x Citrus trifoliata | |
NADPH-hemoprotein reductase | A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ | Citrus sinensis x Citrus trifoliata |
Organism | Comment | Expression |
---|---|---|
Citrus sinensis x Citrus trifoliata | drought represses CsCYP707A expression 11.1 times with respect to control values. CsCYP707A1 expression slightly increases in roots of citrus subjected to soil flooding whereas it strongly decreases in response to water deficit | down |
Citrus sinensis x Citrus trifoliata | moderate induction of CsCYP707A by depletion of abscisic acid, strong induction by drought of both abscisic acid biosynthesis and catabolism, i.e. CsNCED1, 9-cis-neoxanthin epoxycarotenoid dioxygenase1, and CsCYP707A. Soil flooding induces a 2.5fold increase in CsCYP707A expression with respect to control values. Stress release in soil-flooded seedlings reduces CsCYP707A expression to control values but rewatering induces its expression up to 4.2fold. CsCYP707A1 expression slightly increases in roots of citrus subjected to soil flooding whereas it strongly decreases in response to water deficit | up |
General Information | Comment | Organism |
---|---|---|
metabolism | comparison of abscisic acid (ABA) metabolism and signaling in roots of flooded and water stressed plants of Carrizo citrange reveals that the hormone depletion is linked to the upregulation of CsAOG, involved in abscisic acid glycosyl ester (ABAGE) synthesis, and to a moderate induction of catabolism (CsCYP707A, an ABA 8'-hydroxylase) and buildup of dehydrophaseic acid (DPA). Drought strongly induces both ABA biosynthesis and catabolism (CsNCED1, 9-cis-neoxanthin epoxycarotenoid dioxygenase1, and CsCYP707A) rendering a significant hormone accumulation. In roots of flooded plants, restoration of control abscisic acid levels after stress release is associated to the upregulation of CsBGLU18 (an abscisic acid beta--glycosidase) that cleaves ABAGE | Citrus sinensis x Citrus trifoliata |
physiological function | abscisic acid catabolism is represented by the induction of abscisic acid 8'-hydroxylase CYP707A1, which catalyzes the production of phaseic acid | Citrus sinensis x Citrus trifoliata |