BRENDA - Enzyme Database
show all sequences of 1.14.14.13

Biosynthesis of the unique amino acid side chain of butirosin: possible protective-group chemistry in an acyl carrier protein-mediated pathway

Li, Y.; Llewellyn, N.M.; Giri, R.; Huang, F.; Spencer, J.B.; Chem. Biol. 12, 665-675 (2005)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Bacillus circulans
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38700
-
x * 38700, calculated, x * 40000, SDS-PAGE of recombinant His-tagged protein
Bacillus circulans
40000
-
x * 38700, calculated, x * 40000, SDS-PAGE of recombinant His-tagged protein
Bacillus circulans
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus circulans
Q4H4E5
putative
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-(L-gamma-glutamylamino)butanoyl-[BtrI acyl-carrier protein] + FMNH2 + O2
presence of FMN and O2 are required
719270
Bacillus circulans
4-(L-gamma-glutamylamino)-(2S)-2-hydroxybutanoyl-[BtrI acyl-carrier protein] + FMN + H2O
-
-
-
?
butanoyl-[BtrI acyl-carrier protein] + FMNH2 + O2
-
719270
Bacillus circulans
(2S)-2-hydroxybutanoyl-[BtrI acyl-carrier protein] + FMN + H2O
-
-
-
?
additional information
no substrates: butanoate, butanoyl-CoA, L-glutamate, L-aspartate, gamma-aminobutanoate, and L-ornithine
719270
Bacillus circulans
?
-
-
-
-
octanoyl-[BtrI acyl-carrier protein] + FMNH2 + O2
-
719270
Bacillus circulans
(2S)-2-hydroxyoctanoyl-[BtrI acyl-carrier protein] + FMN + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 38700, calculated, x * 40000, SDS-PAGE of recombinant His-tagged protein
Bacillus circulans
Cofactor
Cofactor
Commentary
Organism
Structure
FMN
-
Bacillus circulans
additional information
no cofactor: FAD
Bacillus circulans
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Bacillus circulans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FMN
-
Bacillus circulans
additional information
no cofactor: FAD
Bacillus circulans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38700
-
x * 38700, calculated, x * 40000, SDS-PAGE of recombinant His-tagged protein
Bacillus circulans
40000
-
x * 38700, calculated, x * 40000, SDS-PAGE of recombinant His-tagged protein
Bacillus circulans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-(L-gamma-glutamylamino)butanoyl-[BtrI acyl-carrier protein] + FMNH2 + O2
presence of FMN and O2 are required
719270
Bacillus circulans
4-(L-gamma-glutamylamino)-(2S)-2-hydroxybutanoyl-[BtrI acyl-carrier protein] + FMN + H2O
-
-
-
?
butanoyl-[BtrI acyl-carrier protein] + FMNH2 + O2
-
719270
Bacillus circulans
(2S)-2-hydroxybutanoyl-[BtrI acyl-carrier protein] + FMN + H2O
-
-
-
?
additional information
no substrates: butanoate, butanoyl-CoA, L-glutamate, L-aspartate, gamma-aminobutanoate, and L-ornithine
719270
Bacillus circulans
?
-
-
-
-
octanoyl-[BtrI acyl-carrier protein] + FMNH2 + O2
-
719270
Bacillus circulans
(2S)-2-hydroxyoctanoyl-[BtrI acyl-carrier protein] + FMN + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 38700, calculated, x * 40000, SDS-PAGE of recombinant His-tagged protein
Bacillus circulans
General Information
General Information
Commentary
Organism
physiological function
involved in biosynthesis of the aminoglycoside antibiotic butirosin
Bacillus circulans
General Information (protein specific)
General Information
Commentary
Organism
physiological function
involved in biosynthesis of the aminoglycoside antibiotic butirosin
Bacillus circulans
Other publictions for EC 1.14.14.13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
719270
Li
Biosynthesis of the unique ami ...
Bacillus circulans
Chem. Biol.
12
665-675
2005
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