Application | Comment | Organism |
---|---|---|
biotechnology | enzymatic activity of P450SMO makes it an attractive biocatalyst for asymmetric synthesis of enantiopure sulfoxides | Rhodococcus sp. |
Cloned (Comment) | Organism |
---|---|
into the pET28a (+) vector and expressed in Escherichia coli BL21 (DE3) | Rhodococcus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
imidazole | at 20 mM, only half of P450SMO activity remains | Rhodococcus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the C-terminal reductase portion of P450SMO comprises a [2Fe2S] ferredoxin center | Rhodococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
87190 | - |
sequence analysis | Rhodococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
gel filtration | Rhodococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-ethoxycoumarin + NADPH + H+ + O2 | mediates the O-dealkylation | Rhodococcus sp. | 7-hydroxycoumarin + NADP+ + H2O + ? | - |
? | |
additional information | no hydroxylation activity towards naphthalene, indene, ethyl benzene, and m-xylene | Rhodococcus sp. | ? | - |
? | |
p-chlorothioanisole + [reduced NADPH-hemoprotein reductase] + O2 | - |
Rhodococcus sp. | ? | - |
? | |
p-fluorothioanisole + [reduced NADPH-hemoprotein reductase] + O2 | - |
Rhodococcus sp. | ? | - |
? | |
p-methoxythioanisole + [reduced NADPH-hemoprotein reductase] + O2 | - |
Rhodococcus sp. | ? | - |
? | |
p-tolyl methyl sulfide + [reduced NADPH-hemoprotein reductase] + O2 | - |
Rhodococcus sp. | ? | - |
? | |
phenyl ethyl sulfide + [reduced NADPH-hemoprotein reductase] + O2 | - |
Rhodococcus sp. | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cytochrome P450 monooxygenase | - |
Rhodococcus sp. |
P450SMO | - |
Rhodococcus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
toward p-chlorothioanisole | Rhodococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
toward p-chlorothioanisole | Rhodococcus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | the C-terminal reductase portion of P450SMO comprises an FMN-binding | Rhodococcus sp. | |
NADH | the C-terminal reductase portion of P450SMO comprises an NADH-binding | Rhodococcus sp. | |
NADPH | - |
Rhodococcus sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rhodococcus sp. | - |
- |
5.17 |