BRENDA - Enzyme Database
show all sequences of 1.14.13.79

Characterisation of two genes encoding cytochrome P450 monooxygenases involved in gibberellin biosynthesis in apple (Malus x domestica Borkh.)

Wang, C.; Bai, M.; Tian, Y.; Tian, W.; J. Hortic. Sci. Biotechnol. 89, 329-337 (2014)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene MdKAO, DNA and amino acid sequence determination and analysis, the sequence contains 7 introns, location on chromosome 2, quantitative real-time PCR enzyme expression analysis
Malus domestica
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ent-kaur-16-en-19-oate + 3 NADPH + 3 H+ + 3 O2
Malus domestica
overall reaction
gibberellin A12 + 3 NADP+ + 4 H2O
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Malus domestica
M9R757
cv. Fuji
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
the enzyme level of MdKAO is significantly lower in the columnar parent, and in F1 progeny with the columnar phenotype, compared to standard parent and F1 progeny with the standard phenotype
Malus domestica
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ent-kaur-16-en-19-oate + 3 NADPH + 3 H+ + 3 O2
overall reaction
745483
Malus domestica
gibberellin A12 + 3 NADP+ + 4 H2O
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
KAO
-
Malus domestica
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Malus domestica
NADPH
-
Malus domestica
Cloned(Commentary) (protein specific)
Commentary
Organism
gene MdKAO, DNA and amino acid sequence determination and analysis, the sequence contains 7 introns, location on chromosome 2, quantitative real-time PCR enzyme expression analysis
Malus domestica
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Malus domestica
NADPH
-
Malus domestica
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ent-kaur-16-en-19-oate + 3 NADPH + 3 H+ + 3 O2
Malus domestica
overall reaction
gibberellin A12 + 3 NADP+ + 4 H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
the enzyme level of MdKAO is significantly lower in the columnar parent, and in F1 progeny with the columnar phenotype, compared to standard parent and F1 progeny with the standard phenotype
Malus domestica
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ent-kaur-16-en-19-oate + 3 NADPH + 3 H+ + 3 O2
overall reaction
745483
Malus domestica
gibberellin A12 + 3 NADP+ + 4 H2O
-
-
-
?
General Information
General Information
Commentary
Organism
physiological function
lower expression of the MdKAO gene is responsible for the reduced concentrations of bioactive gibberellins in the apical tissue of columnar apple stems
Malus domestica
General Information (protein specific)
General Information
Commentary
Organism
physiological function
lower expression of the MdKAO gene is responsible for the reduced concentrations of bioactive gibberellins in the apical tissue of columnar apple stems
Malus domestica
Other publictions for EC 1.14.13.79
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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3
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2
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1
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2
1
1
2
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745483
Wang
-
Characterisation of two genes ...
Malus domestica
J. Hortic. Sci. Biotechnol.
89
329-337
2014
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1
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1
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1
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1
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1
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1
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1
1
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746078
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Arabidopsis thaliana
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2
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2
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2
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2
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2
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15
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2
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4
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4
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2
4
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2
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2
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2
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15
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2
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8
8
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