BRENDA - Enzyme Database
show all sequences of 1.14.11.31

Dioxygenases catalyze the O-demethylation steps of morphine biosynthesis in opium poppy.

Hagel, J.M.; Facchini, P.J.; Nat. Chem. Biol. 6, 273-275 (2010)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
His6-tagged protein is produced in Escherichia coli
Papaver somniferum
Inhibitors
Inhibitors
Commentary
Organism
Structure
thebaine
-
Papaver somniferum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0154
-
oripavine
pH 7.4, 30C
Papaver somniferum
0.0164
-
2-oxoglutarate
pH 7.4, 30C
Papaver somniferum
0.0203
-
thebaine
pH 7.4, 30C
Papaver somniferum
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
required
Papaver somniferum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
oripavine + 2-oxoglutarate + O2
Papaver somniferum
a step of morphine biosynthesis
morphinone + formaldehyde + succinate + CO2
-
-
?
thebaine + 2-oxoglutarate + O2
Papaver somniferum
a step of morphine biosynthesis
neopinone + formaldehyde + succinate + CO2
spontaneous conversion to codeinone
-
?
Organism
Organism
UniProt
Commentary
Textmining
Papaver somniferum
-
high-morphine varieties (i.e. L, 11 and 40) and a mutant variety (i.e. T) that accumulates high levels of thebaine and oripavine, but lacks morphine and codeine
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Papaver somniferum
Reaction
Reaction
Commentary
Organism
Reaction ID
thebaine + 2-oxoglutarate + O2 = neopinone + formaldehyde + succinate + CO2
spontaneous conversion of neopinione to codeinone
Papaver somniferum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
additional information
no activity with (S)-reticuline, (S)-scoulerine, papaverine, (S)-corytuberine, pavine, salutaridine, noscapine, codeine
700357
Papaver somniferum
?
-
-
-
?
oripavine + 2-oxoglutarate + O2
a step of morphine biosynthesis
700357
Papaver somniferum
morphinone + formaldehyde + succinate + CO2
-
-
-
?
oripavine + 2-oxoglutarate + O2
alkyl hydroxylation proceeds through a radical mechanism involving an iron-oxo intermediate, followed by the elimination of formaldehyde
700357
Papaver somniferum
morphinone + formaldehyde + succinate + CO2
-
-
-
?
thebaine + 2-oxoglutarate + O2
a step of morphine biosynthesis
700357
Papaver somniferum
neopinone + formaldehyde + succinate + CO2
spontaneous conversion to codeinone
-
-
?
thebaine + 2-oxoglutarate + O2
85% of the activity with oripavine. Alkyl hydroxylation proceeds through a radical mechanism involving an iron-oxo intermediate, followed by the elimination of formaldehyde
700357
Papaver somniferum
neopinone + formaldehyde + succinate + CO2
spontaneous conversion to codeinone
-
-
?
Synonyms
Synonyms
Commentary
Organism
T6ODM
-
Papaver somniferum
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Papaver somniferum
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0081
-
2-oxoglutarate
pH 7.4, 30C
Papaver somniferum
0.017
-
thebaine
pH 7.4, 30C
Papaver somniferum
0.0191
-
oripavine
pH 7.4, 30C
Papaver somniferum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Papaver somniferum
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.518
-
thebaine
pH 7.4, 30C
Papaver somniferum
Cloned(Commentary) (protein specific)
Commentary
Organism
His6-tagged protein is produced in Escherichia coli
Papaver somniferum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
thebaine
-
Papaver somniferum
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.518
-
thebaine
pH 7.4, 30C
Papaver somniferum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0154
-
oripavine
pH 7.4, 30C
Papaver somniferum
0.0164
-
2-oxoglutarate
pH 7.4, 30C
Papaver somniferum
0.0203
-
thebaine
pH 7.4, 30C
Papaver somniferum
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
required
Papaver somniferum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
oripavine + 2-oxoglutarate + O2
Papaver somniferum
a step of morphine biosynthesis
morphinone + formaldehyde + succinate + CO2
-
-
?
thebaine + 2-oxoglutarate + O2
Papaver somniferum
a step of morphine biosynthesis
neopinone + formaldehyde + succinate + CO2
spontaneous conversion to codeinone
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Papaver somniferum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
additional information
no activity with (S)-reticuline, (S)-scoulerine, papaverine, (S)-corytuberine, pavine, salutaridine, noscapine, codeine
700357
Papaver somniferum
?
-
-
-
?
oripavine + 2-oxoglutarate + O2
a step of morphine biosynthesis
700357
Papaver somniferum
morphinone + formaldehyde + succinate + CO2
-
-
-
?
oripavine + 2-oxoglutarate + O2
alkyl hydroxylation proceeds through a radical mechanism involving an iron-oxo intermediate, followed by the elimination of formaldehyde
700357
Papaver somniferum
morphinone + formaldehyde + succinate + CO2
-
-
-
?
thebaine + 2-oxoglutarate + O2
a step of morphine biosynthesis
700357
Papaver somniferum
neopinone + formaldehyde + succinate + CO2
spontaneous conversion to codeinone
-
-
?
thebaine + 2-oxoglutarate + O2
85% of the activity with oripavine. Alkyl hydroxylation proceeds through a radical mechanism involving an iron-oxo intermediate, followed by the elimination of formaldehyde
700357
Papaver somniferum
neopinone + formaldehyde + succinate + CO2
spontaneous conversion to codeinone
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Papaver somniferum
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0081
-
2-oxoglutarate
pH 7.4, 30C
Papaver somniferum
0.017
-
thebaine
pH 7.4, 30C
Papaver somniferum
0.0191
-
oripavine
pH 7.4, 30C
Papaver somniferum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Papaver somniferum
General Information
General Information
Commentary
Organism
malfunction
virus-induced gene silencing of T6ODM in opium poppy efficiently blocks metabolism at thebaine
Papaver somniferum
General Information (protein specific)
General Information
Commentary
Organism
malfunction
virus-induced gene silencing of T6ODM in opium poppy efficiently blocks metabolism at thebaine
Papaver somniferum
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
492.9
-
2-oxoglutarate
pH 7.4, 30C
Papaver somniferum
837.1
-
thebaine
pH 7.4, 30C
Papaver somniferum
1243
-
oripavine
pH 7.4, 30C
Papaver somniferum
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
492.9
-
2-oxoglutarate
pH 7.4, 30C
Papaver somniferum
837.1
-
thebaine
pH 7.4, 30C
Papaver somniferum
1243
-
oripavine
pH 7.4, 30C
Papaver somniferum
Other publictions for EC 1.14.11.31
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743439
Hashemi
-
Production and gene expressio ...
Papaver orientale
Plant Cell
125
31-41
2016
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
1
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
743555
Pandey
Endophytes of opium poppy dif ...
Papaver somniferum
Planta
243
1097-1114
2016
-
-
-
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
725537
Farrow
Dioxygenases catalyze O-demeth ...
Papaver somniferum
J. Biol. Chem.
288
28997-29012
2013
-
-
1
-
-
-
-
-
-
1
-
1
-
4
-
-
-
-
-
-
-
-
10
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
10
-
1
-
-
-
1
-
-
-
-
3
3
-
-
-
700357
Hagel
Dioxygenases catalyze the O-de ...
Papaver somniferum
Nat. Chem. Biol.
6
273-275
2010
-
-
1
-
-
-
1
3
-
1
-
2
-
4
-
-
1
1
-
-
-
-
5
-
1
1
-
-
3
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
1
3
-
1
-
2
-
-
-
1
-
-
-
-
5
-
1
-
-
3
1
-
-
-
-
1
1
-
3
3