Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
T387A | the mutant shows 15fold increased turnover at limiting O2 concentrations compared to the wild type enzyme | Homo sapiens |
T387N | the mutant shows about 2fold reduced turnover at limiting O2 concentrations compared to the wild type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0007 | - |
2-oxoglutarate | mutant enzyme T387N, at pH 7.0 and 37°C | Homo sapiens | |
0.0008 | - |
DLDLEALAPYIPADDDFQL | wild type isoform PHD2, at pH 7.0 and 37°C | Homo sapiens | |
0.0009 | - |
2-oxoglutarate | wild type isoform PHD2, at pH 7.0 and 37°C | Homo sapiens | |
0.0014 | - |
DLDLEALAPYIPADDDFQL | mutant enzyme T387N, at pH 7.0 and 37°C | Homo sapiens | |
0.0041 | - |
DLDLEALAPYIPADDDFQL | mutant enzyme T387A, at pH 7.0 and 37°C | Homo sapiens | |
0.012 | - |
2-oxoglutarate | mutant enzyme T387A, at pH 7.0 and 37°C | Homo sapiens | |
0.1 | - |
O2 | mutant enzyme T387A, at pH 7.0 and 37°C | Homo sapiens | |
0.53 | - |
O2 | wild type isoform PHD2, at pH 7.0 and 37°C | Homo sapiens | |
0.76 | - |
O2 | mutant enzyme T387N, at pH 7.0 and 37°C | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hypoxia-inducible factor-L-proline + 2-oxoglutarate + O2 | Homo sapiens | - |
hypoxia-inducible factor-trans-4-hydroxy-L-proline + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9GZT9 | - |
- |
Purification (Comment) | Organism |
---|---|
GSTrap column chromatography and Hitrap benzamidine column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DLDLEALAPYIPADDDFQL + 2-oxoglutarate + O2 | - |
Homo sapiens | DLDLEALA-((4R)-4-hydroxy-L-proline)-YIPADDDFQL + succinate + CO2 | - |
? | |
hypoxia-inducible factor-L-proline + 2-oxoglutarate + O2 | - |
Homo sapiens | hypoxia-inducible factor-trans-4-hydroxy-L-proline + succinate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 27785, QStar-XL hybrid quadrupole-TOF mass spectrometry | Homo sapiens |
? | x * 27787, calculated from amino acid sequence | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
Hif-prolyl hydroxylase | - |
Homo sapiens |
PHD2 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
DLDLEALAPYIPADDDFQL | mutant enzyme T387N, at pH 7.0 and 37°C | Homo sapiens | |
0.017 | - |
2-oxoglutarate | mutant enzyme T387N, at pH 7.0 and 37°C | Homo sapiens | |
0.032 | - |
DLDLEALAPYIPADDDFQL | wild type isoform PHD2, at pH 7.0 and 37°C | Homo sapiens | |
0.038 | - |
2-oxoglutarate | wild type isoform PHD2, at pH 7.0 and 37°C | Homo sapiens | |
0.063 | - |
O2 | mutant enzyme T387N, at pH 7.0 and 37°C | Homo sapiens | |
0.133 | - |
O2 | wild type isoform PHD2, at pH 7.0 and 37°C | Homo sapiens | |
0.483 | - |
O2 | mutant enzyme T387A, at pH 7.0 and 37°C | Homo sapiens | |
0.548 | - |
2-oxoglutarate | mutant enzyme T387A, at pH 7.0 and 37°C | Homo sapiens | |
0.733 | - |
DLDLEALAPYIPADDDFQL | mutant enzyme T387A, at pH 7.0 and 37°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0083 | - |
O2 | mutant enzyme T387N, at pH 7.0 and 37°C | Homo sapiens | |
0.25 | - |
O2 | wild type isoform PHD2, at pH 7.0 and 37°C | Homo sapiens | |
5 | - |
O2 | mutant enzyme T387A, at pH 7.0 and 37°C | Homo sapiens | |
11.7 | - |
DLDLEALAPYIPADDDFQL | mutant enzyme T387N, at pH 7.0 and 37°C | Homo sapiens | |
25 | - |
2-oxoglutarate | mutant enzyme T387N, at pH 7.0 and 37°C | Homo sapiens | |
40 | - |
DLDLEALAPYIPADDDFQL | wild type isoform PHD2, at pH 7.0 and 37°C | Homo sapiens | |
45 | - |
2-oxoglutarate | mutant enzyme T387A, at pH 7.0 and 37°C | Homo sapiens | |
45 | - |
2-oxoglutarate | wild type isoform PHD2, at pH 7.0 and 37°C | Homo sapiens | |
183 | - |
DLDLEALAPYIPADDDFQL | mutant enzyme T387A, at pH 7.0 and 37°C | Homo sapiens |