Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.11.29 extracted from

  • Pektas, S.; Taabazuing, C.Y.; Knapp, M.J.
    Increased turnover at limiting O2 concentrations by the Thr387 -> Ala variant of HIF-prolyl hydroxylase PHD2 (2015), Biochemistry, 54, 2851-2857 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
T387A the mutant shows 15fold increased turnover at limiting O2 concentrations compared to the wild type enzyme Homo sapiens
T387N the mutant shows about 2fold reduced turnover at limiting O2 concentrations compared to the wild type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0007
-
2-oxoglutarate mutant enzyme T387N, at pH 7.0 and 37°C Homo sapiens
0.0008
-
DLDLEALAPYIPADDDFQL wild type isoform PHD2, at pH 7.0 and 37°C Homo sapiens
0.0009
-
2-oxoglutarate wild type isoform PHD2, at pH 7.0 and 37°C Homo sapiens
0.0014
-
DLDLEALAPYIPADDDFQL mutant enzyme T387N, at pH 7.0 and 37°C Homo sapiens
0.0041
-
DLDLEALAPYIPADDDFQL mutant enzyme T387A, at pH 7.0 and 37°C Homo sapiens
0.012
-
2-oxoglutarate mutant enzyme T387A, at pH 7.0 and 37°C Homo sapiens
0.1
-
O2 mutant enzyme T387A, at pH 7.0 and 37°C Homo sapiens
0.53
-
O2 wild type isoform PHD2, at pH 7.0 and 37°C Homo sapiens
0.76
-
O2 mutant enzyme T387N, at pH 7.0 and 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hypoxia-inducible factor-L-proline + 2-oxoglutarate + O2 Homo sapiens
-
hypoxia-inducible factor-trans-4-hydroxy-L-proline + succinate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9GZT9
-
-

Purification (Commentary)

Purification (Comment) Organism
GSTrap column chromatography and Hitrap benzamidine column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DLDLEALAPYIPADDDFQL + 2-oxoglutarate + O2
-
Homo sapiens DLDLEALA-((4R)-4-hydroxy-L-proline)-YIPADDDFQL + succinate + CO2
-
?
hypoxia-inducible factor-L-proline + 2-oxoglutarate + O2
-
Homo sapiens hypoxia-inducible factor-trans-4-hydroxy-L-proline + succinate + CO2
-
?

Subunits

Subunits Comment Organism
? x * 27785, QStar-XL hybrid quadrupole-TOF mass spectrometry Homo sapiens
? x * 27787, calculated from amino acid sequence Homo sapiens

Synonyms

Synonyms Comment Organism
Hif-prolyl hydroxylase
-
Homo sapiens
PHD2
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.015
-
DLDLEALAPYIPADDDFQL mutant enzyme T387N, at pH 7.0 and 37°C Homo sapiens
0.017
-
2-oxoglutarate mutant enzyme T387N, at pH 7.0 and 37°C Homo sapiens
0.032
-
DLDLEALAPYIPADDDFQL wild type isoform PHD2, at pH 7.0 and 37°C Homo sapiens
0.038
-
2-oxoglutarate wild type isoform PHD2, at pH 7.0 and 37°C Homo sapiens
0.063
-
O2 mutant enzyme T387N, at pH 7.0 and 37°C Homo sapiens
0.133
-
O2 wild type isoform PHD2, at pH 7.0 and 37°C Homo sapiens
0.483
-
O2 mutant enzyme T387A, at pH 7.0 and 37°C Homo sapiens
0.548
-
2-oxoglutarate mutant enzyme T387A, at pH 7.0 and 37°C Homo sapiens
0.733
-
DLDLEALAPYIPADDDFQL mutant enzyme T387A, at pH 7.0 and 37°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0083
-
O2 mutant enzyme T387N, at pH 7.0 and 37°C Homo sapiens
0.25
-
O2 wild type isoform PHD2, at pH 7.0 and 37°C Homo sapiens
5
-
O2 mutant enzyme T387A, at pH 7.0 and 37°C Homo sapiens
11.7
-
DLDLEALAPYIPADDDFQL mutant enzyme T387N, at pH 7.0 and 37°C Homo sapiens
25
-
2-oxoglutarate mutant enzyme T387N, at pH 7.0 and 37°C Homo sapiens
40
-
DLDLEALAPYIPADDDFQL wild type isoform PHD2, at pH 7.0 and 37°C Homo sapiens
45
-
2-oxoglutarate mutant enzyme T387A, at pH 7.0 and 37°C Homo sapiens
45
-
2-oxoglutarate wild type isoform PHD2, at pH 7.0 and 37°C Homo sapiens
183
-
DLDLEALAPYIPADDDFQL mutant enzyme T387A, at pH 7.0 and 37°C Homo sapiens