Literature summary for 1.11.1.8 extracted from

Kaur, P.; Patil, H.; Bhanushali, P.; Badgujar, S.; Gupta, A.
Purification of recombinant human thyroid peroxidase (hTPO) from AD293 mammalian cells (2018), Int. J. Biol. Macromol., 106, 87-94 .

Search for more literature for 1.11.1.8

Application

Application	Comment	Organism
synthesis	expression and purification of recombinant protein from AD-293 cells unsing the aminoterminal cDNA sequence of luciferase protein from Gaussia princeps for secretion. A simplified purification procedure consists of a couple of anionexchange chromatography columns	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expression in AD-293 cell	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
180	-	pH 7.4, 25°C	Homo sapiens

Storage Stability

Storage Stability	Organism
-70°C, purified recombinant protein treated with 600 microM hemin, stable for for 4 years	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
guaiacol + H2O2	-	Homo sapiens	3,3'-dimethoxy-4,4'-biphenoquinone + H2O	-	?

Subunits

Subunits	Comment	Organism
?	x * 105000, SDS-PAGE of recombinant protein	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
heme	heme prosthetic group of TPO is stable and gives a A412/A280ratio of 0.38	Homo sapiens

pI Value

Organism	Comment	pI Value Maximum	pI Value
Homo sapiens	calculated	-	6.1

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Release 2023.1 (January 2023)