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Literature summary for 1.1.99.29 extracted from

  • Staudigl, P.; Krondorfer, I.; Haltrich, D.; Peterbauer, C.K.
    Pyranose dehydrogenase from Agaricus campestris and Agaricus xanthoderma: characterization and applications in carbohydrate conversions (2013), Biomolecules, 3, 535-552.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Agaricus xanthodermus
expressed in Pichia pastoris Agaricus campestris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
ferrocenium ion with D-glucose as cosubstrate, at pH 8.5 and 30°C Agaricus xanthodermus
0.09
-
2,6-dichlorophenolindophenol with D-glucose as cosubstrate, at pH 4.0 and 30°C Agaricus xanthodermus
0.11
-
2,6-dichlorophenolindophenol with D-glucose as cosubstrate, at pH 4.0 and 30°C Agaricus campestris
0.12
-
1,4-benzoquinone with D-glucose as cosubstrate, at pH 4.0 and 30°C Agaricus campestris
0.35
-
D-glucose with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Agaricus campestris
0.49
-
D-glucose with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Agaricus xanthodermus
1.19
-
ferrocenium ion with D-glucose as cosubstrate, at pH 8.5 and 30°C Agaricus campestris
1.44
-
D-xylose with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Agaricus xanthodermus
3.25
-
1,4-benzoquinone with D-glucose as cosubstrate, at pH 4.0 and 30°C Agaricus xanthodermus
4.16
-
L-arabinose with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Agaricus xanthodermus
4.19
-
D-xylose with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Agaricus campestris
4.23
-
L-arabinose with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Agaricus campestris
7.13
-
D-galactose with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Agaricus campestris
53.16
-
lactose with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Agaricus campestris
74.99
-
D-galactose with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Agaricus xanthodermus
293.8
-
lactose with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Agaricus xanthodermus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61800
-
-
Agaricus campestris
62300
-
-
Agaricus xanthodermus
75000
-
1 * 75000, SDS-PAGE Agaricus xanthodermus
98000
-
1 * 98000, SDS-PAGE Agaricus campestris

Organism

Organism UniProt Comment Textmining
Agaricus campestris V5NDL4
-
-
Agaricus xanthodermus V5NC32
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Agaricus xanthodermus
glycoprotein
-
Agaricus campestris

Purification (Commentary)

Purification (Comment) Organism
phenyl Sepharose column chromatography, phenyl source column chromatography, DEAE Sepharose chromatography and gel filtration Agaricus xanthodermus
phenyl Sepharose column chromatography, phenyl source column chromatography, DEAE Sepharose chromatography and gel filtration Agaricus campestris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactose + ferrocenium ion
-
Agaricus xanthodermus 2-dehydro-D-galactose + ferrocene
-
?
D-galactose + ferrocenium ion
-
Agaricus campestris 2-dehydro-D-galactose + ferrocene
-
?
D-glucose + 1,4-benzoquinone
-
Agaricus xanthodermus 2-dehydro-D-glucose + ?
-
?
D-glucose + 1,4-benzoquinone
-
Agaricus campestris 2-dehydro-D-glucose + ?
-
?
D-glucose + 2,6-dichlorophenolindophenol
-
Agaricus xanthodermus 2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol
-
?
D-glucose + 2,6-dichlorophenolindophenol
-
Agaricus campestris 2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol
-
?
D-glucose + ferrocenium ion
-
Agaricus xanthodermus 2-dehydro-D-glucose + ferrocene
-
?
D-glucose + ferrocenium ion
-
Agaricus campestris 2-dehydro-D-glucose + ferrocene
-
?
D-xylose + ferrocenium ion
-
Agaricus xanthodermus 2-dehydro-D-xylose + ferrocene
-
?
D-xylose + ferrocenium ion
-
Agaricus campestris 2-dehydro-D-xylose + ferrocene
-
?
L-arabinose + ferrocenium ion
-
Agaricus xanthodermus 2-dehydro-L-arabinose + ferrocene
-
?
L-arabinose + ferrocenium ion
-
Agaricus campestris 2-dehydro-L-arabinose + ferrocene
-
?
lactose + ferrocenium ion 48% of the lactose is converted and yields 88% lactobionic acid and 12% 2-dehydrolactose Agaricus campestris ? + ferrocene
-
?
lactose + ferrocenium ion the enzyme converts lactose to nearly 100%, producing 69% lactobionic acid and 31% 2-dehydrolactose Agaricus xanthodermus ? + ferrocene
-
?

Subunits

Subunits Comment Organism
monomer 1 * 75000, SDS-PAGE Agaricus xanthodermus
monomer 1 * 98000, SDS-PAGE Agaricus campestris
monomer 1 * 61800, calculated from amino acid sequence Agaricus campestris
monomer 1 * 62300, calculated from amino acid sequence Agaricus xanthodermus

Synonyms

Synonyms Comment Organism
PDH
-
Agaricus xanthodermus
PDH
-
Agaricus campestris

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.05
-
L-arabinose with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Agaricus campestris
3.12
-
lactose with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Agaricus campestris
4.1
-
D-glucose with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Agaricus campestris
5.23
-
D-galactose with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Agaricus campestris
6.37
-
D-xylose with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Agaricus campestris
7.65
-
2,6-dichlorophenolindophenol with D-glucose as cosubstrate, at pH 4.0 and 30°C Agaricus xanthodermus
10.56
-
2,6-dichlorophenolindophenol with D-glucose as cosubstrate, at pH 4.0 and 30°C Agaricus campestris
12.89
-
1,4-benzoquinone with D-glucose as cosubstrate, at pH 4.0 and 30°C Agaricus xanthodermus
13.02
-
D-glucose with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Agaricus xanthodermus
19.92
-
ferrocenium ion with D-glucose as cosubstrate, at pH 8.5 and 30°C Agaricus campestris
22.07
-
ferrocenium ion with D-glucose as cosubstrate, at pH 8.5 and 30°C Agaricus xanthodermus
22.9
-
L-arabinose with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Agaricus xanthodermus
24.65
-
lactose with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Agaricus xanthodermus
24.77
-
D-galactose with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Agaricus xanthodermus
29.16
-
D-xylose with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Agaricus xanthodermus
34.82
-
1,4-benzoquinone with D-glucose as cosubstrate, at pH 4.0 and 30°C Agaricus campestris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
using 1,4-benzoquinone as cosubstrate Agaricus xanthodermus
5
-
using 1,4-benzoquinone as cosubstrate Agaricus campestris
8
-
using ferrocenium ion as cosubstrate Agaricus xanthodermus
8
-
using ferrocenium ion as cosubstrate Agaricus campestris

Cofactor

Cofactor Comment Organism Structure
FAD
-
Agaricus xanthodermus
FAD
-
Agaricus campestris

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.1
-
lactose with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Agaricus xanthodermus
0.1
-
lactose with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Agaricus campestris
0.7
-
D-galactose with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Agaricus campestris
0.7
-
L-arabinose with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Agaricus campestris
1.5
-
D-xylose with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Agaricus campestris
4
-
1,4-benzoquinone with D-glucose as cosubstrate, at pH 4.0 and 30°C Agaricus xanthodermus
5
-
D-galactose with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Agaricus xanthodermus
5.5
-
L-arabinose with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Agaricus xanthodermus
11.7
-
D-glucose with ferrocenium ion as cosubstrate ,at pH 7.5 and 30°C Agaricus campestris
16.7
-
ferrocenium ion with D-glucose as cosubstrate, at pH 8.5 and 30°C Agaricus campestris
20.3
-
D-xylose with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Agaricus xanthodermus
26.6
-
D-glucose with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C Agaricus xanthodermus
85
-
2,6-dichlorophenolindophenol with D-glucose as cosubstrate, at pH 4.0 and 30°C Agaricus xanthodermus
96
-
2,6-dichlorophenolindophenol with D-glucose as cosubstrate, at pH 4.0 and 30°C Agaricus campestris
302.8
-
1,4-benzoquinone with D-glucose as cosubstrate, at pH 4.0 and 30°C Agaricus campestris
735.7
-
ferrocenium ion with D-glucose as cosubstrate, at pH 8.5 and 30°C Agaricus xanthodermus