BRENDA - Enzyme Database
show all sequences of 1.1.99.29

Pyranose dehydrogenase from Agaricus campestris and Agaricus xanthoderma: characterization and applications in carbohydrate conversions

Staudigl, P.; Krondorfer, I.; Haltrich, D.; Peterbauer, C.K.; Biomolecules 3, 535-552 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Pichia pastoris
Agaricus campestris
expressed in Pichia pastoris
Agaricus xanthodermus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.03
-
ferrocenium ion
with D-glucose as cosubstrate, at pH 8.5 and 30°C
Agaricus xanthodermus
0.09
-
2,6-dichlorophenolindophenol
with D-glucose as cosubstrate, at pH 4.0 and 30°C
Agaricus xanthodermus
0.11
-
2,6-dichlorophenolindophenol
with D-glucose as cosubstrate, at pH 4.0 and 30°C
Agaricus campestris
0.12
-
1,4-benzoquinone
with D-glucose as cosubstrate, at pH 4.0 and 30°C
Agaricus campestris
0.35
-
D-glucose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus campestris
0.49
-
D-glucose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus xanthodermus
1.19
-
ferrocenium ion
with D-glucose as cosubstrate, at pH 8.5 and 30°C
Agaricus campestris
1.44
-
D-xylose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus xanthodermus
3.25
-
1,4-benzoquinone
with D-glucose as cosubstrate, at pH 4.0 and 30°C
Agaricus xanthodermus
4.16
-
L-arabinose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus xanthodermus
4.19
-
D-xylose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus campestris
4.23
-
L-arabinose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus campestris
7.13
-
D-galactose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus campestris
53.16
-
lactose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus campestris
74.99
-
D-galactose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus xanthodermus
293.8
-
lactose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus xanthodermus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
61800
-
-
Agaricus campestris
62300
-
-
Agaricus xanthodermus
75000
-
1 * 75000, SDS-PAGE
Agaricus xanthodermus
98000
-
1 * 98000, SDS-PAGE
Agaricus campestris
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Agaricus campestris
V5NDL4
-
-
Agaricus xanthodermus
V5NC32
-
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
glycoprotein
-
Agaricus campestris
glycoprotein
-
Agaricus xanthodermus
Purification (Commentary)
Commentary
Organism
phenyl Sepharose column chromatography, phenyl source column chromatography, DEAE Sepharose chromatography and gel filtration
Agaricus campestris
phenyl Sepharose column chromatography, phenyl source column chromatography, DEAE Sepharose chromatography and gel filtration
Agaricus xanthodermus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-galactose + ferrocenium ion
-
740138
Agaricus xanthodermus
2-dehydro-D-galactose + ferrocene
-
-
-
?
D-galactose + ferrocenium ion
-
740138
Agaricus campestris
2-dehydro-D-galactose + ferrocene
-
-
-
?
D-glucose + 1,4-benzoquinone
-
740138
Agaricus xanthodermus
2-dehydro-D-glucose + ?
-
-
-
?
D-glucose + 1,4-benzoquinone
-
740138
Agaricus campestris
2-dehydro-D-glucose + ?
-
-
-
?
D-glucose + 2,6-dichlorophenolindophenol
-
740138
Agaricus xanthodermus
2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-glucose + 2,6-dichlorophenolindophenol
-
740138
Agaricus campestris
2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-glucose + ferrocenium ion
-
740138
Agaricus xanthodermus
2-dehydro-D-glucose + ferrocene
-
-
-
?
D-glucose + ferrocenium ion
-
740138
Agaricus campestris
2-dehydro-D-glucose + ferrocene
-
-
-
?
D-xylose + ferrocenium ion
-
740138
Agaricus xanthodermus
2-dehydro-D-xylose + ferrocene
-
-
-
?
D-xylose + ferrocenium ion
-
740138
Agaricus campestris
2-dehydro-D-xylose + ferrocene
-
-
-
?
L-arabinose + ferrocenium ion
-
740138
Agaricus xanthodermus
2-dehydro-L-arabinose + ferrocene
-
-
-
?
L-arabinose + ferrocenium ion
-
740138
Agaricus campestris
2-dehydro-L-arabinose + ferrocene
-
-
-
?
lactose + ferrocenium ion
48% of the lactose is converted and yields 88% lactobionic acid and 12% 2-dehydrolactose
740138
Agaricus campestris
? + ferrocene
-
-
-
?
lactose + ferrocenium ion
the enzyme converts lactose to nearly 100%, producing 69% lactobionic acid and 31% 2-dehydrolactose
740138
Agaricus xanthodermus
? + ferrocene
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 61800, calculated from amino acid sequence; 1 * 98000, SDS-PAGE
Agaricus campestris
monomer
1 * 62300, calculated from amino acid sequence; 1 * 75000, SDS-PAGE
Agaricus xanthodermus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.05
-
L-arabinose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus campestris
3.12
-
lactose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus campestris
4.1
-
D-glucose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus campestris
5.23
-
D-galactose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus campestris
6.37
-
D-xylose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus campestris
7.65
-
2,6-dichlorophenolindophenol
with D-glucose as cosubstrate, at pH 4.0 and 30°C
Agaricus xanthodermus
10.56
-
2,6-dichlorophenolindophenol
with D-glucose as cosubstrate, at pH 4.0 and 30°C
Agaricus campestris
12.89
-
1,4-benzoquinone
with D-glucose as cosubstrate, at pH 4.0 and 30°C
Agaricus xanthodermus
13.02
-
D-glucose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus xanthodermus
19.92
-
ferrocenium ion
with D-glucose as cosubstrate, at pH 8.5 and 30°C
Agaricus campestris
22.07
-
ferrocenium ion
with D-glucose as cosubstrate, at pH 8.5 and 30°C
Agaricus xanthodermus
22.9
-
L-arabinose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus xanthodermus
24.65
-
lactose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus xanthodermus
24.77
-
D-galactose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus xanthodermus
29.16
-
D-xylose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus xanthodermus
34.82
-
1,4-benzoquinone
with D-glucose as cosubstrate, at pH 4.0 and 30°C
Agaricus campestris
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
using 1,4-benzoquinone as cosubstrate
Agaricus campestris
5
-
using 1,4-benzoquinone as cosubstrate
Agaricus xanthodermus
8
-
using ferrocenium ion as cosubstrate
Agaricus campestris
8
-
using ferrocenium ion as cosubstrate
Agaricus xanthodermus
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
-
Agaricus campestris
FAD
-
Agaricus xanthodermus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Pichia pastoris
Agaricus campestris
expressed in Pichia pastoris
Agaricus xanthodermus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
-
Agaricus campestris
FAD
-
Agaricus xanthodermus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.03
-
ferrocenium ion
with D-glucose as cosubstrate, at pH 8.5 and 30°C
Agaricus xanthodermus
0.09
-
2,6-dichlorophenolindophenol
with D-glucose as cosubstrate, at pH 4.0 and 30°C
Agaricus xanthodermus
0.11
-
2,6-dichlorophenolindophenol
with D-glucose as cosubstrate, at pH 4.0 and 30°C
Agaricus campestris
0.12
-
1,4-benzoquinone
with D-glucose as cosubstrate, at pH 4.0 and 30°C
Agaricus campestris
0.35
-
D-glucose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus campestris
0.49
-
D-glucose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus xanthodermus
1.19
-
ferrocenium ion
with D-glucose as cosubstrate, at pH 8.5 and 30°C
Agaricus campestris
1.44
-
D-xylose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus xanthodermus
3.25
-
1,4-benzoquinone
with D-glucose as cosubstrate, at pH 4.0 and 30°C
Agaricus xanthodermus
4.16
-
L-arabinose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus xanthodermus
4.19
-
D-xylose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus campestris
4.23
-
L-arabinose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus campestris
7.13
-
D-galactose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus campestris
53.16
-
lactose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus campestris
74.99
-
D-galactose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus xanthodermus
293.8
-
lactose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus xanthodermus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
61800
-
-
Agaricus campestris
62300
-
-
Agaricus xanthodermus
75000
-
1 * 75000, SDS-PAGE
Agaricus xanthodermus
98000
-
1 * 98000, SDS-PAGE
Agaricus campestris
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
glycoprotein
-
Agaricus campestris
glycoprotein
-
Agaricus xanthodermus
Purification (Commentary) (protein specific)
Commentary
Organism
phenyl Sepharose column chromatography, phenyl source column chromatography, DEAE Sepharose chromatography and gel filtration
Agaricus campestris
phenyl Sepharose column chromatography, phenyl source column chromatography, DEAE Sepharose chromatography and gel filtration
Agaricus xanthodermus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-galactose + ferrocenium ion
-
740138
Agaricus xanthodermus
2-dehydro-D-galactose + ferrocene
-
-
-
?
D-galactose + ferrocenium ion
-
740138
Agaricus campestris
2-dehydro-D-galactose + ferrocene
-
-
-
?
D-glucose + 1,4-benzoquinone
-
740138
Agaricus xanthodermus
2-dehydro-D-glucose + ?
-
-
-
?
D-glucose + 1,4-benzoquinone
-
740138
Agaricus campestris
2-dehydro-D-glucose + ?
-
-
-
?
D-glucose + 2,6-dichlorophenolindophenol
-
740138
Agaricus xanthodermus
2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-glucose + 2,6-dichlorophenolindophenol
-
740138
Agaricus campestris
2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-glucose + ferrocenium ion
-
740138
Agaricus xanthodermus
2-dehydro-D-glucose + ferrocene
-
-
-
?
D-glucose + ferrocenium ion
-
740138
Agaricus campestris
2-dehydro-D-glucose + ferrocene
-
-
-
?
D-xylose + ferrocenium ion
-
740138
Agaricus xanthodermus
2-dehydro-D-xylose + ferrocene
-
-
-
?
D-xylose + ferrocenium ion
-
740138
Agaricus campestris
2-dehydro-D-xylose + ferrocene
-
-
-
?
L-arabinose + ferrocenium ion
-
740138
Agaricus xanthodermus
2-dehydro-L-arabinose + ferrocene
-
-
-
?
L-arabinose + ferrocenium ion
-
740138
Agaricus campestris
2-dehydro-L-arabinose + ferrocene
-
-
-
?
lactose + ferrocenium ion
48% of the lactose is converted and yields 88% lactobionic acid and 12% 2-dehydrolactose
740138
Agaricus campestris
? + ferrocene
-
-
-
?
lactose + ferrocenium ion
the enzyme converts lactose to nearly 100%, producing 69% lactobionic acid and 31% 2-dehydrolactose
740138
Agaricus xanthodermus
? + ferrocene
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 61800, calculated from amino acid sequence; 1 * 98000, SDS-PAGE
Agaricus campestris
monomer
1 * 62300, calculated from amino acid sequence; 1 * 75000, SDS-PAGE
Agaricus xanthodermus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.05
-
L-arabinose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus campestris
3.12
-
lactose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus campestris
4.1
-
D-glucose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus campestris
5.23
-
D-galactose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus campestris
6.37
-
D-xylose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus campestris
7.65
-
2,6-dichlorophenolindophenol
with D-glucose as cosubstrate, at pH 4.0 and 30°C
Agaricus xanthodermus
10.56
-
2,6-dichlorophenolindophenol
with D-glucose as cosubstrate, at pH 4.0 and 30°C
Agaricus campestris
12.89
-
1,4-benzoquinone
with D-glucose as cosubstrate, at pH 4.0 and 30°C
Agaricus xanthodermus
13.02
-
D-glucose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus xanthodermus
19.92
-
ferrocenium ion
with D-glucose as cosubstrate, at pH 8.5 and 30°C
Agaricus campestris
22.07
-
ferrocenium ion
with D-glucose as cosubstrate, at pH 8.5 and 30°C
Agaricus xanthodermus
22.9
-
L-arabinose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus xanthodermus
24.65
-
lactose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus xanthodermus
24.77
-
D-galactose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus xanthodermus
29.16
-
D-xylose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus xanthodermus
34.82
-
1,4-benzoquinone
with D-glucose as cosubstrate, at pH 4.0 and 30°C
Agaricus campestris
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
using 1,4-benzoquinone as cosubstrate
Agaricus campestris
5
-
using 1,4-benzoquinone as cosubstrate
Agaricus xanthodermus
8
-
using ferrocenium ion as cosubstrate
Agaricus campestris
8
-
using ferrocenium ion as cosubstrate
Agaricus xanthodermus
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.1
-
lactose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus campestris
0.1
-
lactose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus xanthodermus
0.7
-
D-galactose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus campestris
0.7
-
L-arabinose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus campestris
1.5
-
D-xylose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus campestris
4
-
1,4-benzoquinone
with D-glucose as cosubstrate, at pH 4.0 and 30°C
Agaricus xanthodermus
5
-
D-galactose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus xanthodermus
5.5
-
L-arabinose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus xanthodermus
11.7
-
D-glucose
with ferrocenium ion as cosubstrate ,at pH 7.5 and 30°C
Agaricus campestris
16.7
-
ferrocenium ion
with D-glucose as cosubstrate, at pH 8.5 and 30°C
Agaricus campestris
20.3
-
D-xylose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus xanthodermus
26.6
-
D-glucose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus xanthodermus
85
-
2,6-dichlorophenolindophenol
with D-glucose as cosubstrate, at pH 4.0 and 30°C
Agaricus xanthodermus
96
-
2,6-dichlorophenolindophenol
with D-glucose as cosubstrate, at pH 4.0 and 30°C
Agaricus campestris
302.8
-
1,4-benzoquinone
with D-glucose as cosubstrate, at pH 4.0 and 30°C
Agaricus campestris
735.7
-
ferrocenium ion
with D-glucose as cosubstrate, at pH 8.5 and 30°C
Agaricus xanthodermus
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.1
-
lactose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus campestris
0.1
-
lactose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus xanthodermus
0.7
-
D-galactose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus campestris
0.7
-
L-arabinose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus campestris
1.5
-
D-xylose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus campestris
4
-
1,4-benzoquinone
with D-glucose as cosubstrate, at pH 4.0 and 30°C
Agaricus xanthodermus
5
-
D-galactose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus xanthodermus
5.5
-
L-arabinose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus xanthodermus
11.7
-
D-glucose
with ferrocenium ion as cosubstrate ,at pH 7.5 and 30°C
Agaricus campestris
16.7
-
ferrocenium ion
with D-glucose as cosubstrate, at pH 8.5 and 30°C
Agaricus campestris
20.3
-
D-xylose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus xanthodermus
26.6
-
D-glucose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
Agaricus xanthodermus
85
-
2,6-dichlorophenolindophenol
with D-glucose as cosubstrate, at pH 4.0 and 30°C
Agaricus xanthodermus
96
-
2,6-dichlorophenolindophenol
with D-glucose as cosubstrate, at pH 4.0 and 30°C
Agaricus campestris
302.8
-
1,4-benzoquinone
with D-glucose as cosubstrate, at pH 4.0 and 30°C
Agaricus campestris
735.7
-
ferrocenium ion
with D-glucose as cosubstrate, at pH 8.5 and 30°C
Agaricus xanthodermus
Other publictions for EC 1.1.99.29
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739926
Graf
Characterization of three pyra ...
Leucoagaricus meleagris
Appl. Microbiol. Biotechnol.
101
2879-2891
2017
-
-
1
-
-
-
-
36
-
-
1
-
-
8
-
1
1
-
-
-
-
-
36
1
2
-
1
36
1
-
-
1
-
-
-
-
-
3
3
-
-
-
-
-
-
37
-
-
3
-
-
-
3
3
-
-
-
-
36
3
3
-
3
36
3
-
-
-
-
-
-
-
36
36
740401
Gonaus
Analysis of Agaricus meleagris ...
Leucoagaricus meleagris
Enzyme Microb. Technol.
99
57-66
2017
-
-
1
-
6
-
-
40
-
-
1
-
-
2
-
1
1
-
-
-
-
-
4
1
-
-
-
40
-
-
-
1
-
-
-
-
-
1
1
-
6
-
-
-
-
40
-
-
1
-
-
-
1
1
-
-
-
-
4
1
-
-
-
40
-
-
-
-
-
-
-
-
-
-
741362
Gonaus
Transcription analysis of pyra ...
Agaricus bisporus, Agaricus bisporus DSM 3056
Protein Expr. Purif.
119
36-44
2016
-
-
1
-
-
-
-
13
-
-
-
-
-
6
-
-
1
-
-
1
-
1
17
1
1
-
-
13
1
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
13
-
-
-
-
-
-
-
1
-
1
-
1
17
1
1
-
-
13
1
1
1
-
-
-
-
-
13
13
740437
Graf
Reaction of pyranose dehydroge ...
Leucoagaricus meleagris
FEBS J.
282
4218-4241
2015
-
-
1
-
8
-
-
-
-
-
1
-
-
1
-
1
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
8
-
-
-
-
-
-
-
1
-
-
-
1
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
741112
Yakovleva
Engineering of pyranose dehydr ...
Leucoagaricus meleagris
Phys. Chem. Chem. Phys.
17
9074-9081
2015
-
-
1
-
-
-
-
-
1
-
-
-
-
3
-
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
739955
Krondorfer
Agaricus meleagris pyranose de ...
Leucoagaricus meleagris
Arch. Biochem. Biophys.
558
111-119
2014
-
-
-
-
1
-
-
-
-
-
1
-
-
3
-
1
-
-
-
-
-
-
1
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
740391
Cruys-Bagger
A pyranose dehydrogenase-based ...
Leucoagaricus meleagris
Enzyme Microb. Technol.
58-59
68-74
2014
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
741223
Graf
Pyranose dehydrogenase ligand ...
Leucoagaricus meleagris
PLoS Comput. Biol.
10
e1003995
2014
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
741303
Krondorfer
Engineering of pyranose dehydr ...
Leucoagaricus meleagris
PLoS ONE
9
e91145
2014
-
-
1
-
1
-
-
14
-
-
1
-
-
5
-
1
1
-
-
-
-
-
6
1
-
-
-
12
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
14
-
-
1
-
-
-
1
1
-
-
-
-
6
1
-
-
-
12
-
-
-
-
-
-
-
-
12
12
725611
Graf
Molecular dynamics simulations ...
Leucoagaricus meleagris
J. Comput. Aided Mol. Des.
27
295-304
2013
-
-
-
-
-
-
-
-
-
-
-
1
-
5
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726317
Tan
The 1.6 A crystal structure of ...
Leucoagaricus meleagris
PLoS ONE
8
e53567
2013
-
-
1
1
-
-
-
-
-
-
-
4
-
5
-
-
1
-
-
-
-
-
5
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
4
-
-
-
1
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
739847
Yakovleva
Further insights into the cata ...
Leucoagaricus meleagris
Anal. Chem.
85
9852-9858
2013
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740138
Staudigl
Pyranose dehydrogenase from Ag ...
Agaricus campestris, Agaricus xanthodermus
Biomolecules
3
535-552
2013
-
-
2
-
-
-
-
16
-
-
4
-
-
7
-
2
2
-
-
-
-
-
14
2
-
-
-
16
4
-
-
2
-
-
-
-
-
2
2
-
-
-
-
-
-
16
-
-
4
-
-
-
2
2
-
-
-
-
14
2
-
-
-
16
4
-
-
-
-
-
-
-
16
16
724070
Sygmund
Simple and efficient expressio ...
Leucoagaricus meleagris
Appl. Microbiol. Biotechnol.
94
695-704
2012
-
-
1
-
-
-
-
16
-
-
3
7
-
6
-
1
1
-
-
-
2
-
7
1
-
-
-
16
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
16
-
-
3
7
-
-
1
1
-
-
2
-
7
1
-
-
-
16
-
-
-
-
-
-
-
-
16
16
724834
Yakovleva
-
Recombinant pyranose dehydroge ...
Leucoagaricus meleagris
Electrochem. Commun.
24
120-122
2012
-
-
1
-
-
-
-
-
1
-
-
1
-
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
710969
Pisanelli
Heterologous expression of an ...
Leucoagaricus meleagris, Leucoagaricus meleagris CCBAS 907
Appl. Microbiol. Biotechnol.
86
599-606
2010
-
-
1
-
-
-
-
16
-
-
1
-
-
10
-
1
1
-
-
-
1
-
12
-
-
-
-
16
3
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
16
-
-
1
-
-
-
1
1
-
-
1
-
12
-
-
-
-
16
3
-
-
1
-
-
-
-
16
16
711340
Zafar
Wiring of pyranose dehydrogena ...
Leucoagaricus meleagris
Bioelectrochemistry
80
38-42
2010
-
1
-
-
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
724043
Peterbauer
Pyranose dehydrogenases: Bioch ...
Agaricus xanthodermus, Chlorophyllum rhacodes, Leucoagaricus meleagris
Appl. Microbiol. Biotechnol.
85
837-848
2010
-
-
-
-
-
-
-
14
3
-
3
44
-
5
-
3
-
-
-
-
-
-
44
3
-
-
-
14
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
14
3
-
3
44
-
-
3
-
-
-
-
-
44
3
-
-
-
14
-
-
-
-
-
-
-
-
14
14
695788
Peterbauer
-
Pyranose dehydrogenases: bioch ...
Agaricus bisporus, Agaricus campestris, Agaricus xanthodermus, Chlorophyllum rhacodes, Leucoagaricus meleagris
Appl. Microbiol. Biotechnol.
146
491-499
2009
-
-
-
-
-
-
-
14
-
-
-
-
-
7
-
5
-
-
-
-
14
-
155
5
-
-
-
14
-
-
-
5
-
-
-
-
-
-
7
-
-
-
-
-
-
42
-
-
-
-
-
-
7
-
-
-
42
-
155
7
-
-
-
42
-
-
-
-
-
-
-
-
-
-
686261
Kittl
Molecular cloning of three pyr ...
Leucoagaricus meleagris
Curr. Genet.
53
117-127
2008
-
1
1
-
-
-
-
-
1
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687883
Sygmund
Characterization of pyranose d ...
Leucoagaricus meleagris
J. Biotechnol.
133
334-342
2008
1
1
-
-
-
-
9
20
1
11
2
-
-
3
-
1
1
-
-
2
-
1
21
1
1
-
1
-
2
1
1
1
-
4
-
1
1
-
1
-
-
-
-
9
-
20
1
11
2
-
-
-
1
1
-
2
-
1
21
1
1
-
1
-
2
1
1
4
-
-
-
-
-
-
686372
Tasca
-
Amperometric biosensors for de ...
Agaricus xanthodermus, Leucoagaricus meleagris
Electroanalysis
19
294-302
2007
-
2
-
-
-
-
-
18
2
-
-
-
-
2
-
-
2
-
-
-
-
-
18
-
-
-
-
-
2
4
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
18
2
-
-
-
-
-
-
2
-
-
-
-
18
-
-
-
-
-
2
4
-
-
-
-
-
-
-
-
686685
Kujawa
Properties of pyranose dehydro ...
Agaricus xanthodermus
FEBS J.
274
879-894
2007
-
1
-
-
-
-
-
21
1
-
1
-
-
4
-
1
1
-
-
-
-
1
22
1
1
-
-
-
3
1
1
1
-
1
-
-
1
-
1
-
-
-
-
-
-
21
1
-
1
-
-
-
1
1
-
-
-
1
22
1
1
-
-
-
3
1
1
1
-
-
-
-
-
-
675469
Sedmera
-
New biotransformations of some ...
Leucoagaricus meleagris
J. Mol. Catal. B
41
32-42
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
644548
Volc
-
C-3 oxidation of non-reducing ...
Leucoagaricus meleagris, Leucoagaricus meleagris CCBAS 907
J. Mol. Catal. B
17
91-100
2002
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
1
1
1
14
-
1
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
1
14
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
644547
Volc
Screening of basidiomycete fun ...
Chlorophyllum rhacodes
Arch. Microbiol.
176
178-186
2001
-
-
-
-
-
1
-
4
1
-
2
-
-
7
-
1
1
-
-
3
1
1
37
1
2
-
-
-
2
-
-
2
-
1
-
-
-
-
2
-
-
1
-
-
-
4
1
-
2
-
-
-
1
1
-
3
1
1
37
1
2
-
-
-
2
-
-
1
-
-
-
-
-
-
644546
Volc
Double oxidation of D-xylose t ...
Agaricus bisporus, Agaricus bisporus 306
Carbohydr. Res.
329
219-225
2000
-
1
-
-
-
-
-
-
1
-
-
-
-
4
-
-
1
-
-
3
-
-
10
-
1
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
3
-
-
10
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
644545
Volc
-
C-2 and C-3 oxidation of D-Glc ...
Agaricus bisporus, Agaricus bisporus U3
Carbohydr. Res.
310
151-156
1998
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
1
-
-
1
-
-
10
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
10
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
644544
Volc
Pyranose 2-dehydrogenase, a no ...
Agaricus bisporus
Arch. Microbiol.
167
119-125
1997
1
-
-
-
-
1
5
-
1
-
2
1
-
3
-
1
1
1
-
2
1
1
30
1
1
-
-
-
2
1
-
1
-
1
-
1
-
-
1
-
-
1
-
5
-
-
1
-
2
1
-
-
1
1
-
2
1
1
30
1
1
-
-
-
2
1
-
1
-
-
-
-
-
-