Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris | Agaricus xanthodermus |
expressed in Pichia pastoris | Agaricus campestris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
ferrocenium ion | with D-glucose as cosubstrate, at pH 8.5 and 30°C | Agaricus xanthodermus | |
0.09 | - |
2,6-dichlorophenolindophenol | with D-glucose as cosubstrate, at pH 4.0 and 30°C | Agaricus xanthodermus | |
0.11 | - |
2,6-dichlorophenolindophenol | with D-glucose as cosubstrate, at pH 4.0 and 30°C | Agaricus campestris | |
0.12 | - |
1,4-benzoquinone | with D-glucose as cosubstrate, at pH 4.0 and 30°C | Agaricus campestris | |
0.35 | - |
D-glucose | with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Agaricus campestris | |
0.49 | - |
D-glucose | with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Agaricus xanthodermus | |
1.19 | - |
ferrocenium ion | with D-glucose as cosubstrate, at pH 8.5 and 30°C | Agaricus campestris | |
1.44 | - |
D-xylose | with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Agaricus xanthodermus | |
3.25 | - |
1,4-benzoquinone | with D-glucose as cosubstrate, at pH 4.0 and 30°C | Agaricus xanthodermus | |
4.16 | - |
L-arabinose | with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Agaricus xanthodermus | |
4.19 | - |
D-xylose | with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Agaricus campestris | |
4.23 | - |
L-arabinose | with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Agaricus campestris | |
7.13 | - |
D-galactose | with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Agaricus campestris | |
53.16 | - |
lactose | with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Agaricus campestris | |
74.99 | - |
D-galactose | with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Agaricus xanthodermus | |
293.8 | - |
lactose | with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Agaricus xanthodermus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61800 | - |
- |
Agaricus campestris |
62300 | - |
- |
Agaricus xanthodermus |
75000 | - |
1 * 75000, SDS-PAGE | Agaricus xanthodermus |
98000 | - |
1 * 98000, SDS-PAGE | Agaricus campestris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agaricus campestris | V5NDL4 | - |
- |
Agaricus xanthodermus | V5NC32 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Agaricus xanthodermus |
glycoprotein | - |
Agaricus campestris |
Purification (Comment) | Organism |
---|---|
phenyl Sepharose column chromatography, phenyl source column chromatography, DEAE Sepharose chromatography and gel filtration | Agaricus xanthodermus |
phenyl Sepharose column chromatography, phenyl source column chromatography, DEAE Sepharose chromatography and gel filtration | Agaricus campestris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose + ferrocenium ion | - |
Agaricus xanthodermus | 2-dehydro-D-galactose + ferrocene | - |
? | |
D-galactose + ferrocenium ion | - |
Agaricus campestris | 2-dehydro-D-galactose + ferrocene | - |
? | |
D-glucose + 1,4-benzoquinone | - |
Agaricus xanthodermus | 2-dehydro-D-glucose + ? | - |
? | |
D-glucose + 1,4-benzoquinone | - |
Agaricus campestris | 2-dehydro-D-glucose + ? | - |
? | |
D-glucose + 2,6-dichlorophenolindophenol | - |
Agaricus xanthodermus | 2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-glucose + 2,6-dichlorophenolindophenol | - |
Agaricus campestris | 2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-glucose + ferrocenium ion | - |
Agaricus xanthodermus | 2-dehydro-D-glucose + ferrocene | - |
? | |
D-glucose + ferrocenium ion | - |
Agaricus campestris | 2-dehydro-D-glucose + ferrocene | - |
? | |
D-xylose + ferrocenium ion | - |
Agaricus xanthodermus | 2-dehydro-D-xylose + ferrocene | - |
? | |
D-xylose + ferrocenium ion | - |
Agaricus campestris | 2-dehydro-D-xylose + ferrocene | - |
? | |
L-arabinose + ferrocenium ion | - |
Agaricus xanthodermus | 2-dehydro-L-arabinose + ferrocene | - |
? | |
L-arabinose + ferrocenium ion | - |
Agaricus campestris | 2-dehydro-L-arabinose + ferrocene | - |
? | |
lactose + ferrocenium ion | 48% of the lactose is converted and yields 88% lactobionic acid and 12% 2-dehydrolactose | Agaricus campestris | ? + ferrocene | - |
? | |
lactose + ferrocenium ion | the enzyme converts lactose to nearly 100%, producing 69% lactobionic acid and 31% 2-dehydrolactose | Agaricus xanthodermus | ? + ferrocene | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 75000, SDS-PAGE | Agaricus xanthodermus |
monomer | 1 * 98000, SDS-PAGE | Agaricus campestris |
monomer | 1 * 61800, calculated from amino acid sequence | Agaricus campestris |
monomer | 1 * 62300, calculated from amino acid sequence | Agaricus xanthodermus |
Synonyms | Comment | Organism |
---|---|---|
PDH | - |
Agaricus xanthodermus |
PDH | - |
Agaricus campestris |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.05 | - |
L-arabinose | with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Agaricus campestris | |
3.12 | - |
lactose | with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Agaricus campestris | |
4.1 | - |
D-glucose | with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Agaricus campestris | |
5.23 | - |
D-galactose | with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Agaricus campestris | |
6.37 | - |
D-xylose | with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Agaricus campestris | |
7.65 | - |
2,6-dichlorophenolindophenol | with D-glucose as cosubstrate, at pH 4.0 and 30°C | Agaricus xanthodermus | |
10.56 | - |
2,6-dichlorophenolindophenol | with D-glucose as cosubstrate, at pH 4.0 and 30°C | Agaricus campestris | |
12.89 | - |
1,4-benzoquinone | with D-glucose as cosubstrate, at pH 4.0 and 30°C | Agaricus xanthodermus | |
13.02 | - |
D-glucose | with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Agaricus xanthodermus | |
19.92 | - |
ferrocenium ion | with D-glucose as cosubstrate, at pH 8.5 and 30°C | Agaricus campestris | |
22.07 | - |
ferrocenium ion | with D-glucose as cosubstrate, at pH 8.5 and 30°C | Agaricus xanthodermus | |
22.9 | - |
L-arabinose | with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Agaricus xanthodermus | |
24.65 | - |
lactose | with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Agaricus xanthodermus | |
24.77 | - |
D-galactose | with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Agaricus xanthodermus | |
29.16 | - |
D-xylose | with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Agaricus xanthodermus | |
34.82 | - |
1,4-benzoquinone | with D-glucose as cosubstrate, at pH 4.0 and 30°C | Agaricus campestris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
using 1,4-benzoquinone as cosubstrate | Agaricus xanthodermus |
5 | - |
using 1,4-benzoquinone as cosubstrate | Agaricus campestris |
8 | - |
using ferrocenium ion as cosubstrate | Agaricus xanthodermus |
8 | - |
using ferrocenium ion as cosubstrate | Agaricus campestris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Agaricus xanthodermus | |
FAD | - |
Agaricus campestris |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
lactose | with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Agaricus xanthodermus | |
0.1 | - |
lactose | with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Agaricus campestris | |
0.7 | - |
D-galactose | with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Agaricus campestris | |
0.7 | - |
L-arabinose | with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Agaricus campestris | |
1.5 | - |
D-xylose | with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Agaricus campestris | |
4 | - |
1,4-benzoquinone | with D-glucose as cosubstrate, at pH 4.0 and 30°C | Agaricus xanthodermus | |
5 | - |
D-galactose | with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Agaricus xanthodermus | |
5.5 | - |
L-arabinose | with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Agaricus xanthodermus | |
11.7 | - |
D-glucose | with ferrocenium ion as cosubstrate ,at pH 7.5 and 30°C | Agaricus campestris | |
16.7 | - |
ferrocenium ion | with D-glucose as cosubstrate, at pH 8.5 and 30°C | Agaricus campestris | |
20.3 | - |
D-xylose | with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Agaricus xanthodermus | |
26.6 | - |
D-glucose | with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C | Agaricus xanthodermus | |
85 | - |
2,6-dichlorophenolindophenol | with D-glucose as cosubstrate, at pH 4.0 and 30°C | Agaricus xanthodermus | |
96 | - |
2,6-dichlorophenolindophenol | with D-glucose as cosubstrate, at pH 4.0 and 30°C | Agaricus campestris | |
302.8 | - |
1,4-benzoquinone | with D-glucose as cosubstrate, at pH 4.0 and 30°C | Agaricus campestris | |
735.7 | - |
ferrocenium ion | with D-glucose as cosubstrate, at pH 8.5 and 30°C | Agaricus xanthodermus |