Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2,3-didehydro-D-glucose + 1,4-benzoquinone
3-dehydro-D-glucose + hydroquinone
2-dehydro-D-xylose + 1,4-benzoquinone
2,3-didehydro-D-xylose + hydroquinone
2-dehydrolactose + acceptor
2,3-didehydrolactose + reduced acceptor
Q3L1D2
Substrates: -
Products: -
?
2-deoxy-D-galactose + acceptor
?
2-deoxy-D-glucose + 1,4-benzoquinone
?
-
Substrates: 49% of the activity with D-glucose
Products: -
?
2-deoxy-D-glucose + acceptor
?
2-deoxy-D-glucose + ferricenium ion
?
-
Substrates: 21% of the activity with D-glucose
Products: -
?
2-hydroxymethylphenyl-beta-D-glucopyranoside + acceptor
?
Substrates: salicin, relative activity towards D-glucose (100%): 78%
Products: -
?
3-dehydro-D-glucose + 1,4-benzoquinone
2,3-didehydro-D-glucose + hydroquinone
-
Substrates: -
Products: -
?
3-fluoro-3-deoxy-D-glucose + acceptor
? + reduced acceptor
Substrates: -
Products: -
?
4-hydroxyphenyl-beta-D-glucopyranoside + acceptor
?
Substrates: arbutin
Products: -
?
6-deoxy-D-glucose + acceptor
?
Substrates: relative activity towards D-glucose (100%): 105%
Products: -
?
a pyranoside + acceptor
3,4-didehydropyranoside + reduced acceptor
Substrates: -
Products: -
?
a pyranoside + acceptor
a 3,4-didehydropyranoside + reduced acceptor
a pyranoside + acceptor
a 3-dehydropyranoside (or 3,4-didehydropyranoside) + reduced acceptor
a pyranoside + acceptor
a 3-dehydropyranoside + reduced acceptor
aldopyranose + 1,4-benzoquinone
2-aldoketose + hydroquinone
-
Substrates: more active as electron acceptor than 2,6-dichlorophenolindophenol or ferricyanide, less active than 3,5-di-tert-butyl-1,2-benzoquinone, several oligosaccharides and glycopyranosides are converted to the corresponding 2-aldoketoses/aldosuloses
Products: -
?
aldopyranose + 2,6-dichlorophenolindophenol
2-aldoketose + reduced 2,6-dichlorophenolindophenol
-
Substrates: 65.9% of the activity with 1,4-benzoquinone as electron acceptor, several oligosaccharides and glycopyranosides are converted to the corresponding 2-aldoketoses/aldosuloses
Products: -
?
aldopyranose + 3,5-di-tert-butyl-1,2-benzoquinone
2-aldoketose + reduced 3,5-di-tert-butyl-1,2-benzoquinone
aldopyranose + ferricyanide
2-aldoketose + ferrocyanide
-
Substrates: 10.4% of the activity with 1,4-benzoquinone as electron acceptor, several oligosaccharides and glycopyranosides are converted to the corresponding 2-aldoketoses/aldosuloses
Products: -
?
aldopyranose + quinone
2-aldoketose + hydroquinone
-
Substrates: C-2 specific sugar oxidoreductase, highly nonspecific, several oligosaccharides and glycopyranosides are converted to the corresponding 2-aldoketoses/aldosuloses
Products: -
?
alpha,alpha-trehalose + 1,4-benzoquinone
3,3'-didehydro-alpha,alpha-trehalose + hydroquinone
alpha,alpha-trehalose + acceptor
3,3'-didehydro-alpha,alpha-trehalose + reduced acceptor
Substrates: relative activity towards D-glucose (100%): 27%
Products: -
?
beta-lactose + acceptor
?
-
Substrates: -
Products: -
?
beta-lactose + ferricenium hexafluorophosphate
?
Q3L1D2
Substrates: -
Products: -
?
cellobiitol + acceptor
?
Substrates: relative activity towards D-glucose (100%): 77%
Products: -
?
cellobiose + 1,4-benzoquinone
?
cellobiose + 2,6-dichlorophenolindophenol
2-dehydro-cellobiose + reduced 2,6-dichlorophenolindophenol
-
Substrates: -
Products: -
?
cellobiose + acceptor
?
Substrates: relative activity towards D-glucose (100%): 70%
Products: -
?
cellobiose + Fe3+
?
-
Substrates: -
Products: -
?
cellobiose + ferricenium ion
?
-
Substrates: 7% of the activity with D-glucose
Products: -
?
cellobiose + ferricenium ion
? + ferrocene
Substrates: -
Products: -
?
cellobiose + ferrocenium ion
2-dehydro-cellobiose + ferrocene
cellotetraose + acceptor
?
Substrates: relative activity towards D-glucose (100%): 14%
Products: -
?
cellotriose + 1,4-benzoquinone
?
-
Substrates: 56% of the activity with D-glucose
Products: -
?
D-allose + 1,4-benzoquinone
2-dehydro-D-allose + hydroquinone
-
Substrates: 30% of the activity with D-glucose
Products: -
?
D-allose + acceptor
?
Substrates: relative activity towards D-glucose (100%): 15%
Products: -
?
D-allose + ferricenium ion
? + ferrocene
Substrates: -
Products: -
?
D-arabino-2-hexosulose + 1,4-benzoquinone
?
-
Substrates: 59% of the activity with D-glucose
Products: -
?
D-arabinose + 1,4-benzoquinone
2-dehydro-D-arabinose + hydroquinone
-
Substrates: 18% of the activity with D-glucose
Products: -
?
D-arabinose + ferricenium hexafluorophosphate
?
Q3L1D2
Substrates: -
Products: -
?
D-cellobiose + acceptor
?
-
Substrates: -
Products: -
?
D-fructose + acceptor
?
Substrates: -
Products: -
?
D-fructose + Fe3+
?
-
Substrates: -
Products: -
?
D-fructose + ferricenium ion
? + ferrocene
Substrates: -
Products: -
?
D-galactose + 1,4-benzoquinone
2-dehydro-D-galactose + hydroquinone
D-galactose + 1,4-benzoquinone
?
-
Substrates: 66% of the activity with D-glucose
Products: -
?
D-galactose + acceptor
2-dehydro-D-galactose + reduced acceptor
Substrates: relative activity towards D-glucase (100%): 99%
Products: -
?
D-galactose + benzoquinone
2,3-didehydro-D-galactose + hydroquinone
Q3L1D2
Substrates: -
Products: two main reaction products
?
D-galactose + benzoquinone
2-dehydro-D-galactose + hydroquinone
D-galactose + Fe3+
2-dehydro-D-galactose + Fe2+ + H+
-
Substrates: -
Products: -
?
D-galactose + ferricenium ion
?
-
Substrates: 60% of the activity with D-glucose
Products: -
?
D-galactose + ferricenium ion
? + ferrocene
D-galactose + ferrocenium ion
2-dehydro-D-galactose + ferrocene
D-glucono-1,5-lactone + 1,4-benzoquinone
?
D-glucono-1,5-lactone + acceptor
?
Substrates: relative activity towards D-glucose (100%): 42%
Products: -
?
D-glucono-1,5-lactone + ferricenium ion
?
-
Substrates: 8% of the activity with D-glucose
Products: -
?
D-glucosamine + acceptor
?
-
Substrates: -
Products: -
?
D-glucose
3-dehydro-D-glucose + Fe2+ + H+
-
Substrates: pH 8.5
Products: -
?
D-glucose + 1,4-benzoquinone
2,3-didehydro-D-glucose + hydroquinone
D-glucose + 1,4-benzoquinone
2-dehydro-D-glucose + ?
D-glucose + 1,4-benzoquinone
2-dehydro-D-glucose + hydroquinone
D-glucose + 1,4-benzoquinone
3-dehydro-D-glucose + hydroquinone
D-glucose + 1,4-benzoquinone
?
D-glucose + 2 1,4-benzoquinone
2,3-didehydro-D-glucose + 2 hydroquinone
-
Substrates: -
Products: -
?
D-glucose + 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
2-dehydro-D-glucose + reduced 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
D-glucose + 2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid
2-dehydro-D-glucose + ?
Substrates: -
Products: -
?
D-glucose + 2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid
3-dehydro-D-glucose + ?
Substrates: -
Products: -
?
D-glucose + 2,5-chloro-1,4-benzoquinone
?
-
Substrates: pH 5
Products: -
?
D-glucose + 2,6-dichloroindophenol
?
D-glucose + 2,6-dichlorophenolindophenol
2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol
D-glucose + 2-(hydroxymethyl)-6-methoxy-1,4-benzoquinone
?
Q3L1D2
Substrates: -
Products: -
?
D-glucose + 2-chloro-1,4-benzoquinone
?
D-glucose + 3,5-di-t-butyl-1,2-benzoquinone
?
Q3L1D2
Substrates: -
Products: -
?
D-glucose + 3,5-di-tert-butyl-1,4-benzoquinone
?
-
Substrates: pH 6
Products: -
?
D-glucose + 3-ethylbenzthiazolin-6-sulfonic acid cation radical
?
Q3L1D2
Substrates: -
Products: -
?
D-glucose + acceptor
2,3-didehydro-D-glucose + reduced acceptor
-
Substrates: dioxidation at C2 and C3
Products: -
?
D-glucose + acceptor
2-dehydro-D-glucose + reduced acceptor
D-glucose + acceptor
3-dehydro-D-glucose + reduced acceptor
D-glucose + acceptor
?
Substrates: relative activity towards D-glucose (100%): 100%
Products: -
?
D-glucose + benzoquinone
2,3-didehydro-D-glucose + hydroquinone
D-glucose + CuSO4
?
Q3L1D2
Substrates: -
Products: -
?
D-glucose + dimethylaminomethyl-ferricenium
?
Q3L1D2
Substrates: -
Products: -
?
D-glucose + Fe3+
3-dehydro-D-glucose + Fe2+ + H+
Q3L1D2
Substrates: -
Products: -
?
D-glucose + ferricenium ion
2-dehydro-D-glucose + ferrocene
D-glucose + ferricenium ion
3-dehydro-D-glucose + ferrocene
D-glucose + ferricyanide
?
Q3L1D2
Substrates: -
Products: -
?
D-glucose + ferrocenium ion
2-dehydro-D-glucose + ferrocene
D-glucose + ferrocenium ion
3-dehydro-D-glucose + ferrocene
D-glucose + methyl-1,4-benzoquinone
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
Substrates: -
Products: -
?
D-glucose + tetrachloro-1,4-benzoquinone
?
Q3L1D2
Substrates: -
Products: -
?
D-glucose + tetrafluoro-1,4-benzoquinone
?
Q3L1D2
Substrates: -
Products: -
?
D-gulose + acceptor
?
Substrates: relative activity towards D-glucose (100%): 7%
Products: -
?
D-lyxose + 1,4-benzoquinone
?
-
Substrates: 5% of the activity with D-glucose
Products: -
?
D-lyxose + ferricenium ion
?
-
Substrates: 5% of the activity with D-glucose
Products: -
?
D-maltoheptaose + acceptor
?
-
Substrates: -
Products: -
?
D-maltopentaose + acceptor
?
-
Substrates: -
Products: -
?
D-maltose + Fe3+
?
-
Substrates: -
Products: -
?
D-maltose + ferricenium hexafluorophosphate
?
Q3L1D2
Substrates: -
Products: -
?
D-maltose + ferricenium ion
? + ferrocene
Substrates: -
Products: -
?
D-maltotriose + acceptor
?
-
Substrates: -
Products: -
?
D-maltotriose + Fe3+
?
-
Substrates: -
Products: -
?
D-maltotriose + ferricenium hexafluorophosphate
?
Q3L1D2
Substrates: -
Products: -
?
D-mannose + 1,4-benzoquinone
?
-
Substrates: 32% of the activity with D-glucose
Products: -
?
D-mannose + acceptor
2-dehydro-D-mannose + reduced acceptor
Substrates: -
Products: -
?
D-mannose + Fe3+
?
-
Substrates: -
Products: -
?
D-mannose + ferricenium ion
?
-
Substrates: 26% of the activity with D-glucose
Products: -
?
D-mannose + ferricenium ion
? + ferrocene
D-mannose + ferrocenium ion
2-dehydro-D-mannose + ferrocene
D-ribose + 1,4-benzoquinone
2-dehydro-D-ribose + hydroquinone
-
Substrates: 34% of the activity with D-glucose
Products: -
?
D-ribose + acceptor
?
Substrates: relative activity towards D-glucose (100%): 19%
Products: -
?
D-ribose + Fe3+
?
-
Substrates: -
Products: -
?
D-ribose + ferricenium ion
? + ferrocene
Substrates: -
Products: -
?
D-ribulose + acceptor
?
Substrates: relative activity towards D-glucose (100%): 17%
Products: -
?
D-sorbose + acceptor
?
Substrates: -
Products: -
?
D-tagatose + acceptor
?
Substrates: relative activity towards D-glucoase (100%): 67%
Products: -
?
D-talose + acceptor
?
Substrates: relative activity towards D-glucose (100%): 10%
Products: -
?
D-talose + Fe3+
?
-
Substrates: -
Products: -
?
D-talose + ferricenium ion
? + ferrocene
Substrates: -
Products: -
?
D-trehalose + acceptor
?
-
Substrates: -
Products: -
?
D-xylobiose + ferricenium ion
?
-
Substrates: -
Products: -
r
D-xylose + 1,4-benzoquinone
2-dehydro-D-xylose + hydroquinone
D-xylose + 1,4-benzoquinone
?
-
Substrates: 72% of the activity with D-glucose
Products: -
?
D-xylose + 2 1,4-benzoquinone
2,3-didehydro-D-xylose + 2 hydroquinone
Substrates: -
Products: -
?
D-xylose + Fe3+
?
-
Substrates: -
Products: -
?
D-xylose + ferricenium hexafluorophosphate
?
Q3L1D2
Substrates: -
Products: -
?
D-xylose + ferricenium ion
?
-
Substrates: 81% of the activity with D-glucose
Products: -
?
D-xylose + ferricenium ion
? + ferrocene
D-xylose + ferrocenium ion
2-dehydro-D-xylose + ferrocene
D-xylose + ferrocenium ion
? + ferrocene
Substrates: -
Products: -
?
erlose + 1,4-benzoquinone
3-dehydroerlose + hydroquinone
gentiobiose + ferricenium hexafluorophosphate
?
Q3L1D2
Substrates: -
Products: -
?
L-arabinose + 1,4-benzoquinone
2-dehydro-L-arabinose + hydroquinone
-
Substrates: 113% of the activity with D-glucose
Products: -
?
L-arabinose + 1,4-benzoquinone
?
-
Substrates: 54% of the activity with D-glucose
Products: -
?
L-arabinose + Fe3+
?
-
Substrates: -
Products: -
?
L-arabinose + ferricenium ion
?
-
Substrates: 72% of the activity with D-glucose
Products: -
?
L-arabinose + ferricenium ion
? + ferrocene
Substrates: -
Products: -
?
L-arabinose + ferrocenium ion
2-dehydro-L-arabinose + ferrocene
L-arabinose + ferrocenium ion
? + ferrocene
Substrates: -
Products: -
?
L-glucose + 1,4-benzoquinone
2-dehydro-L-glucose + hydroquinone
-
Substrates: 61% of the activity with D-glucose
Products: -
?
L-glucose + acceptor
?
Substrates: relative activity towards D-glucose (100%): 42%
Products: -
?
L-glucose + ferricenium ion
?
-
Substrates: 11% of the activity with D-glucose
Products: -
?
L-sorbose + 1,4-benzoquinone
2-dehydro-L-sorbose + hydroquinone
-
Substrates: 10% of the activity with D-glucose
Products: -
?
L-sorbose + acceptor
?
Substrates: relative activity towards D-glucose (100%): 5%
Products: -
?
L-sorbose + Fe3+
?
-
Substrates: -
Products: -
?
L-sorbose + ferricenium ion
? + ferrocene
Substrates: -
Products: -
?
L-xylobiose + Fe3+
?
-
Substrates: -
Products: -
?
lactose + 1,4-benzoquinone
?
-
Substrates: 21% of the activity with D-glucose
Products: -
?
lactose + acceptor
2-dehydrolactose + reduced acceptor
Q3L1D2
Substrates: -
Products: -
?
lactose + acceptor
?
Substrates: relative activity towards D-glucose (100%): 15%
Products: -
?
lactose + acceptor
lactobiono-1,5-lactone + 2-dehydrolactose + 2,3-didehydrolactose + reduced acceptor
lactose + acceptor
lactobiono-1,5-lactone + reduced acceptor
Q3L1D2
Substrates: -
Products: -
?
lactose + Fe3+
?
-
Substrates: -
Products: -
?
lactose + ferricenium ion
? + ferrocene
lactose + ferrocenium ion
2-dehydro-lactose + ferrocene
Substrates: -
Products: -
?
lactose + ferrocenium ion
? + ferrocene
maltose + 1,4-benzoquinone
?
maltose + ferricenium ion
?
-
Substrates: 45% of the activity with D-glucose
Products: -
?
maltose + ferricenium ion
? + ferrocene
Substrates: -
Products: -
?
maltose + ferrocenium ion
2-dehydro-maltose + ferrocene
Substrates: -
Products: -
?
maltotetraose + acceptor
?
Substrates: relative activity towards D-glucose (100%): 9%
Products: -
?
maltotriose + 1,4-benzoquinone
?
maltotriose + acceptor
?
Substrates: relative activity towards D-glucose (100%): 12%
Products: -
?
maltotriose + ferricenium ion
?
maltotriose + ferricenium ion
? + ferrocene
melezitose + acceptor
?
Substrates: relative activity towards D-glucose (100%): 13%
Products: -
?
melibiose + acceptor
?
Substrates: relative activity towards D-glucose (100%): 48%
Products: -
?
melizitose + 1,4-benzoquinone
3-dehydromelizitose + hydroquinone
methyl alpha-D-glucopyranoside + acceptor
?
Substrates: relative activity towards D-glucose (100%): 107%
Products: -
?
methyl alpha-D-mannopyranoside + acceptor
?
Substrates: relative activity towards D-glucose (100%): 5%
Products: -
?
methyl beta-D-glucopyranoside + acceptor
?
Substrates: relative activity towards D-glucose (100%): 58%
Products: -
?
methyl-alpha-D-galactopyranose + acceptor
?
Substrates: relative activity towards D-glucose (100%): 17%
Products: -
?
methyl-alpha-D-galactopyranoside + 1,4-benzoquinone
?
-
Substrates: 11% of the activity with D-glucose
Products: -
?
methyl-alpha-D-galactopyranoside + 1,4-benzoquinone
methyl-alpha-3-dehydro-D-galactopyranoside + hydroquinone
methyl-alpha-D-galactopyranoside + ferricenium ion
?
-
Substrates: 8% of the activity with D-glucose
Products: -
?
methyl-alpha-D-glucopyranoside + 1,4-benzoquinone
?
-
Substrates: 141% of the activity with D-glucose
Products: -
?
methyl-alpha-D-glucopyranoside + 1,4-benzoquinone
methyl-alpha-3-dehydro-D-glucopyranoside + hydroquinone
methyl-alpha-D-glucopyranoside + ferricenium ion
?
-
Substrates: 143% of the activity with D-glucose
Products: -
?
methyl-alpha-D-glucopyranoside + ferricenium ion
? + ferrocene
Substrates: -
Products: -
?
methyl-beta-D-glucopyranoside + 1,4-benzoquinone
?
-
Substrates: 30% of the activity with D-glucose
Products: -
?
methyl-beta-D-glucopyranoside + 1,4-benzoquinone
methyl-beta-3-dehydro-D-glucopyranoside + hydroquinone
methyl-beta-D-glucopyranoside + ferricenium ion
?
-
Substrates: 17% of the activity with D-glucose
Products: -
?
palatinose + acceptor
?
Substrates: relative activity towards D-glucose (100%): 89%
Products: -
?
pyranose + acceptor
2,3-didehydropyranose + reduced acceptor
pyranose + acceptor
2-dehydropyranose (or 3-dehydropyranose or 2,3-didehydropyranose) + reduced acceptor
pyranose + acceptor
2-dehydropyranose + reduced acceptor
pyranose + acceptor
3-dehydropyranose + reduced acceptor
pyranoside + acceptor
3,4-didehydropyranoside + reduced acceptor
raffinose + ferrocenium ion
?
Substrates: -
Products: -
?
sucrose + 1,4-benzoquinone
3'-dehydrosucrose + hydroquinone
-
Substrates: quinone-dependent PDH oxidizes sucrose exclusively at C-3 of its terminal glucopyranosyl moiety, 11% of the activity with D-glucose
Products: oxidation of sucrose at C-3 of its terminal glucopyranosyl moiety
?
sucrose + 1,4-benzoquinone
?
sucrose + acceptor
3'-dehydrosucrose + reduced acceptor
Substrates: relative activity towards D-glucose (100%): 46%
Products: -
?
sucrose + acceptor
?
-
Substrates: -
Products: -
?
sucrose + ferricenium ion
?
-
Substrates: 6% of the activity with D-glucose
Products: -
?
trehalose + 1,4-benzoquinone
?
trehalose + ferricenium ion
?
-
Substrates: 8% of the activity with D-glucose
Products: -
?
xylobiose + acceptor
?
Substrates: -
Products: -
?
xylobiose + ferricenium ion
? + ferrocene
Substrates: -
Products: -
?
xylooligosaccharide + acceptor
?
additional information
?
-
2,3-didehydro-D-glucose + 1,4-benzoquinone
3-dehydro-D-glucose + hydroquinone
Q3L1D2
Substrates: -
Products: -
?
2,3-didehydro-D-glucose + 1,4-benzoquinone
3-dehydro-D-glucose + hydroquinone
-
Substrates: -
Products: -
?
2,3-didehydro-D-glucose + 1,4-benzoquinone
3-dehydro-D-glucose + hydroquinone
Substrates: -
Products: -
?
2-dehydro-D-xylose + 1,4-benzoquinone
2,3-didehydro-D-xylose + hydroquinone
Q3L1D2
Substrates: -
Products: -
?
2-dehydro-D-xylose + 1,4-benzoquinone
2,3-didehydro-D-xylose + hydroquinone
-
Substrates: -
Products: -
?
2-dehydro-D-xylose + 1,4-benzoquinone
2,3-didehydro-D-xylose + hydroquinone
Substrates: -
Products: -
?
2-deoxy-D-galactose + acceptor
?
-
Substrates: -
Products: -
?
2-deoxy-D-galactose + acceptor
?
Substrates: relative activity towards D-glucose (100%): 31%
Products: -
?
2-deoxy-D-glucose + acceptor
?
-
Substrates: -
Products: -
?
2-deoxy-D-glucose + acceptor
?
Substrates: relative activity towards D-glucose (100%): 27%
Products: -
?
a pyranoside + acceptor
a 3,4-didehydropyranoside + reduced acceptor
Q3L1D2
Substrates: -
Products: -
?
a pyranoside + acceptor
a 3,4-didehydropyranoside + reduced acceptor
-
Substrates: -
Products: -
?
a pyranoside + acceptor
a 3,4-didehydropyranoside + reduced acceptor
Substrates: -
Products: -
?
a pyranoside + acceptor
a 3-dehydropyranoside (or 3,4-didehydropyranoside) + reduced acceptor
-
Substrates: -
Products: -
?
a pyranoside + acceptor
a 3-dehydropyranoside (or 3,4-didehydropyranoside) + reduced acceptor
-
Substrates: -
Products: -
?
a pyranoside + acceptor
a 3-dehydropyranoside (or 3,4-didehydropyranoside) + reduced acceptor
-
Substrates: -
Products: -
?
a pyranoside + acceptor
a 3-dehydropyranoside (or 3,4-didehydropyranoside) + reduced acceptor
-
Substrates: -
Products: -
?
a pyranoside + acceptor
a 3-dehydropyranoside + reduced acceptor
Substrates: -
Products: -
?
a pyranoside + acceptor
a 3-dehydropyranoside + reduced acceptor
Q3L1D2
Substrates: -
Products: -
?
a pyranoside + acceptor
a 3-dehydropyranoside + reduced acceptor
-
Substrates: -
Products: -
?
a pyranoside + acceptor
a 3-dehydropyranoside + reduced acceptor
Substrates: -
Products: -
?
a pyranoside + acceptor
a 3-dehydropyranoside + reduced acceptor
Substrates: -
Products: -
?
a pyranoside + acceptor
a 3-dehydropyranoside + reduced acceptor
Substrates: -
Products: 3-dehydropyranoside or 3,4-didehydropyranoside
?
aldopyranose + 3,5-di-tert-butyl-1,2-benzoquinone
2-aldoketose + reduced 3,5-di-tert-butyl-1,2-benzoquinone
-
Substrates: 4.3fold higher activity than with 1,4-benzoquinone as electron acceptor, several oligosaccharides and glycopyranosides are converted to the corresponding 2-aldoketoses/aldosuloses
Products: -
?
aldopyranose + 3,5-di-tert-butyl-1,2-benzoquinone
2-aldoketose + reduced 3,5-di-tert-butyl-1,2-benzoquinone
Q3L1D2
Substrates: -
Products: -
?
alpha,alpha-trehalose + 1,4-benzoquinone
3,3'-didehydro-alpha,alpha-trehalose + hydroquinone
-
Substrates: quinone-dependent PDH oxidizes alpha,alpha-trehalose at C-3 and C-3, 5% of the activity with D-glucose
Products: with 3-dehydro-alpha,alpha-trehalose as reaction intermediate
?
alpha,alpha-trehalose + 1,4-benzoquinone
3,3'-didehydro-alpha,alpha-trehalose + hydroquinone
-
Substrates: quinone-dependent PDH oxidizes alpha,alpha-trehalose at C-3 and C-3, 5% of the activity with D-glucose
Products: with 3-dehydro-alpha,alpha-trehalose as reaction intermediate
?
cellobiose + 1,4-benzoquinone
?
-
Substrates: 94% of the activity with D-glucose
Products: -
?
cellobiose + 1,4-benzoquinone
?
-
Substrates: 13% of the activity with D-glucose
Products: -
?
cellobiose + ferrocenium ion
2-dehydro-cellobiose + ferrocene
Substrates: -
Products: -
?
cellobiose + ferrocenium ion
2-dehydro-cellobiose + ferrocene
Substrates: -
Products: -
?
cellobiose + ferrocenium ion
2-dehydro-cellobiose + ferrocene
Substrates: -
Products: -
?
D-galactose + 1,4-benzoquinone
2-dehydro-D-galactose + hydroquinone
-
Substrates: 88% of the activity with D-glucose
Products: -
?
D-galactose + 1,4-benzoquinone
2-dehydro-D-galactose + hydroquinone
-
Substrates: PDH oxidizes D-galactose exclusively at C-2 to produce D-lyxo-hexos-2-ulose
Products: 2-keto-D-galactose
?
D-galactose + 1,4-benzoquinone
2-dehydro-D-galactose + hydroquinone
-
Substrates: quinone-dependent PDH oxidizes D-galactose exclusively at C-2
Products: -
?
D-galactose + 1,4-benzoquinone
2-dehydro-D-galactose + hydroquinone
-
Substrates: quinone-dependent PDH oxidizes D-galactose exclusively at C-2
Products: -
?
D-galactose + 1,4-benzoquinone
2-dehydro-D-galactose + hydroquinone
-
Substrates: PDH oxidizes D-galactose exclusively at C-2 to produce D-lyxo-hexos-2-ulose
Products: 2-keto-D-galactose
?
D-galactose + acceptor
?
-
Substrates: -
Products: -
?
D-galactose + acceptor
?
Substrates: -
Products: -
?
D-galactose + benzoquinone
2-dehydro-D-galactose + hydroquinone
Q3L1D2
Substrates: -
Products: two main reaction products
?
D-galactose + benzoquinone
2-dehydro-D-galactose + hydroquinone
-
Substrates: -
Products: no 2,3-didehydro-d-galactose is detectable
?
D-galactose + ferricenium ion
? + ferrocene
Substrates: -
Products: -
?
D-galactose + ferricenium ion
? + ferrocene
Substrates: -
Products: -
?
D-galactose + ferricenium ion
? + ferrocene
Substrates: -
Products: -
?
D-galactose + ferrocenium ion
2-dehydro-D-galactose + ferrocene
Substrates: -
Products: -
?
D-galactose + ferrocenium ion
2-dehydro-D-galactose + ferrocene
Substrates: -
Products: -
?
D-galactose + ferrocenium ion
2-dehydro-D-galactose + ferrocene
Substrates: -
Products: -
?
D-galactose + ferrocenium ion
2-dehydro-D-galactose + ferrocene
Substrates: -
Products: -
?
D-galactose + ferrocenium ion
2-dehydro-D-galactose + ferrocene
Substrates: -
Products: -
?
D-galactose + ferrocenium ion
2-dehydro-D-galactose + ferrocene
-
Substrates: -
Products: -
?
D-galactose + ferrocenium ion
2-dehydro-D-galactose + ferrocene
Substrates: -
Products: -
?
D-glucono-1,5-lactone + 1,4-benzoquinone
?
-
Substrates: 118% of the activity with D-glucose
Products: -
?
D-glucono-1,5-lactone + 1,4-benzoquinone
?
-
Substrates: 20% of the activity with D-glucose
Products: -
?
D-glucose + 1,4-benzoquinone
2,3-didehydro-D-glucose + hydroquinone
Q3L1D2
Substrates: -
Products: -
?
D-glucose + 1,4-benzoquinone
2,3-didehydro-D-glucose + hydroquinone
-
Substrates: -
Products: -
?
D-glucose + 1,4-benzoquinone
2,3-didehydro-D-glucose + hydroquinone
Substrates: -
Products: -
?
D-glucose + 1,4-benzoquinone
2-dehydro-D-glucose + ?
Substrates: -
Products: -
?
D-glucose + 1,4-benzoquinone
2-dehydro-D-glucose + ?
Substrates: -
Products: -
?
D-glucose + 1,4-benzoquinone
2-dehydro-D-glucose + ?
Substrates: -
Products: -
?
D-glucose + 1,4-benzoquinone
2-dehydro-D-glucose + ?
Substrates: -
Products: -
?
D-glucose + 1,4-benzoquinone
2-dehydro-D-glucose + hydroquinone
Substrates: -
Products: -
?
D-glucose + 1,4-benzoquinone
2-dehydro-D-glucose + hydroquinone
-
Substrates: -
Products: D-arabino-2-hexosulose, 2-ketoglucose
?
D-glucose + 1,4-benzoquinone
2-dehydro-D-glucose + hydroquinone
Substrates: -
Products: -
?
D-glucose + 1,4-benzoquinone
3-dehydro-D-glucose + hydroquinone
-
Substrates: PDH catalyzes the C-2 and C-3 oxidation of D-glucose, quinone-dependent
Products: or 2-dehydro-D-glucose or 2,3-didehydro-D-glucose, products are 2-keto-D-glucose and, preferentially, 3-keto-D-glucose, both are subsequently oxidized by PDH to 2,3-diketo-D-glucose
?
D-glucose + 1,4-benzoquinone
3-dehydro-D-glucose + hydroquinone
-
Substrates: quinone-dependent PDH catalyzes C-2 or, preferentially, C-3 dehydrogenation of D-glucose
Products: or 2-dehydro-D-glucose or 2,3-didehydro-D-glucose
?
D-glucose + 1,4-benzoquinone
3-dehydro-D-glucose + hydroquinone
-
Substrates: quinone-dependent PDH catalyzes C-2 or, preferentially, C-3 dehydrogenation of D-glucose
Products: or 2-dehydro-D-glucose or 2,3-didehydro-D-glucose
?
D-glucose + 1,4-benzoquinone
3-dehydro-D-glucose + hydroquinone
-
Substrates: PDH catalyzes the C-2 and C-3 oxidation of D-glucose, quinone-dependent
Products: or 2-dehydro-D-glucose or 2,3-didehydro-D-glucose, products are 2-keto-D-glucose and, preferentially, 3-keto-D-glucose, both are subsequently oxidized by PDH to 2,3-diketo-D-glucose
?
D-glucose + 1,4-benzoquinone
3-dehydro-D-glucose + hydroquinone
-
Substrates: oxidizes D-glucose exclusively at C-3, D-glucose is the preferred electron donor susbtrate
Products: -
?
D-glucose + 1,4-benzoquinone
3-dehydro-D-glucose + hydroquinone
-
Substrates: simultaneous C-2 and C-3 oxidation of free D-glucose
Products: or 2-dehydro-D-glucose
?
D-glucose + 1,4-benzoquinone
3-dehydro-D-glucose + hydroquinone
-
Substrates: simultaneous C-2 and C-3 oxidation of free D-glucose
Products: or 2-dehydro-D-glucose
?
D-glucose + 1,4-benzoquinone
?
Substrates: -
Products: -
?
D-glucose + 1,4-benzoquinone
?
-
Substrates: -
Products: -
?
D-glucose + 1,4-benzoquinone
?
Substrates: -
Products: -
?
D-glucose + 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
2-dehydro-D-glucose + reduced 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
Substrates: -
Products: -
?
D-glucose + 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
2-dehydro-D-glucose + reduced 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
Substrates: -
Products: -
?
D-glucose + 2,6-dichloroindophenol
?
Q3L1D2
Substrates: -
Products: -
?
D-glucose + 2,6-dichloroindophenol
?
-
Substrates: pH 4
Products: -
?
D-glucose + 2,6-dichlorophenolindophenol
2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol
Substrates: -
Products: -
?
D-glucose + 2,6-dichlorophenolindophenol
2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol
Substrates: -
Products: -
?
D-glucose + 2,6-dichlorophenolindophenol
2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol
Substrates: -
Products: -
?
D-glucose + 2,6-dichlorophenolindophenol
2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol
Substrates: -
Products: -
?
D-glucose + 2,6-dichlorophenolindophenol
2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol
-
Substrates: -
Products: -
?
D-glucose + 2,6-dichlorophenolindophenol
2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol
Substrates: -
Products: -
?
D-glucose + 2-chloro-1,4-benzoquinone
?
Q3L1D2
Substrates: -
Products: -
?
D-glucose + 2-chloro-1,4-benzoquinone
?
Substrates: -
Products: -
?
D-glucose + 2-chloro-1,4-benzoquinone
?
-
Substrates: -
Products: -
?
D-glucose + 2-chloro-1,4-benzoquinone
?
-
Substrates: pH 5
Products: -
?
D-glucose + 2-chloro-1,4-benzoquinone
?
Substrates: -
Products: -
?
D-glucose + acceptor
2-dehydro-D-glucose + reduced acceptor
Substrates: -
Products: -
?
D-glucose + acceptor
2-dehydro-D-glucose + reduced acceptor
Substrates: the enzyme oxidizes D-glucose at both the C2 and C3 position but the C2 position is the primary site of attack
Products: -
?
D-glucose + acceptor
3-dehydro-D-glucose + reduced acceptor
Q3L1D2
Substrates: -
Products: -
?
D-glucose + acceptor
3-dehydro-D-glucose + reduced acceptor
-
Substrates: -
Products: -
?
D-glucose + benzoquinone
2,3-didehydro-D-glucose + hydroquinone
Q3L1D2
Substrates: 3-oxo-D-glucose, 1-alpha-3-dehydro-d-glucopyranose, 1-beta-3-dehydro-d-glucopyranose, 1-alpha-3-dehydro-d-glucopyranose hydrate, 1-beta-3-dehydro-d-glucopyranose hydrate, 2-dehydro-d-glucose, 1-alpha-2,3-bis-dehydro-d-glucopyranose bis-hydrate as minor reaction products
Products: -
?
D-glucose + benzoquinone
2,3-didehydro-D-glucose + hydroquinone
-
Substrates: pH 3
Products: -
?
D-glucose + ferricenium ion
2-dehydro-D-glucose + ferrocene
Substrates: -
Products: -
?
D-glucose + ferricenium ion
2-dehydro-D-glucose + ferrocene
Substrates: -
Products: -
?
D-glucose + ferricenium ion
3-dehydro-D-glucose + ferrocene
Q3L1D2
Substrates: -
Products: -
?
D-glucose + ferricenium ion
3-dehydro-D-glucose + ferrocene
-
Substrates: -
Products: -
?
D-glucose + ferricenium ion
3-dehydro-D-glucose + ferrocene
-
Substrates: oxidizes D-glucose exclusively at C-3, D-glucose is the preferred electron donor susbtrate
Products: -
?
D-glucose + ferricenium ion
3-dehydro-D-glucose + ferrocene
Substrates: -
Products: -
?
D-glucose + ferricenium ion
3-dehydro-D-glucose + ferrocene
Substrates: -
Products: -
?
D-glucose + ferrocenium ion
2-dehydro-D-glucose + ferrocene
Substrates: ferrocenium ion is the preferred electron acceptor
Products: -
?
D-glucose + ferrocenium ion
2-dehydro-D-glucose + ferrocene
Substrates: -
Products: -
?
D-glucose + ferrocenium ion
2-dehydro-D-glucose + ferrocene
Substrates: -
Products: -
?
D-glucose + ferrocenium ion
2-dehydro-D-glucose + ferrocene
-
Substrates: -
Products: -
?
D-glucose + ferrocenium ion
2-dehydro-D-glucose + ferrocene
Substrates: -
Products: -
?
D-glucose + ferrocenium ion
2-dehydro-D-glucose + ferrocene
-
Substrates: -
Products: -
?
D-glucose + ferrocenium ion
3-dehydro-D-glucose + ferrocene
-
Substrates: -
Products: -
?
D-glucose + ferrocenium ion
3-dehydro-D-glucose + ferrocene
Substrates: -
Products: -
?
D-glucose + methyl-1,4-benzoquinone
?
Q3L1D2
Substrates: -
Products: -
?
D-glucose + methyl-1,4-benzoquinone
?
-
Substrates: pH 8
Products: -
?
D-maltose + acceptor
?
-
Substrates: -
Products: -
?
D-maltose + acceptor
?
Substrates: relative activity towards D-glucose (100%): 61%
Products: -
?
D-mannose + acceptor
?
-
Substrates: -
Products: -
?
D-mannose + acceptor
?
Substrates: relative activity towards D-glucose (100%): 24%
Products: -
?
D-mannose + ferricenium ion
? + ferrocene
Substrates: -
Products: -
?
D-mannose + ferricenium ion
? + ferrocene
Substrates: -
Products: -
?
D-mannose + ferrocenium ion
2-dehydro-D-mannose + ferrocene
Substrates: -
Products: -
?
D-mannose + ferrocenium ion
2-dehydro-D-mannose + ferrocene
Substrates: -
Products: -
?
D-xylose + 1,4-benzoquinone
2-dehydro-D-xylose + hydroquinone
-
Substrates: 116% of the activity with D-glucose
Products: -
?
D-xylose + 1,4-benzoquinone
2-dehydro-D-xylose + hydroquinone
-
Substrates: quinone-dependent PDH oxidizes D-xylose at C-2 to 2-keto-D-xylose and successively at C-3 to 2,3-diketo-D-xylose
Products: or 2,3-didehydro-D-xylose
?
D-xylose + 1,4-benzoquinone
2-dehydro-D-xylose + hydroquinone
-
Substrates: quinone-dependent PDH oxidizes D-xylose at C-2 to 2-keto-D-xylose and successively at C-3 to 2,3-diketo-D-xylose
Products: or 2,3-didehydro-D-xylose
?
D-xylose + 1,4-benzoquinone
2-dehydro-D-xylose + hydroquinone
Q3L1D2
Substrates: -
Products: -
?
D-xylose + 1,4-benzoquinone
2-dehydro-D-xylose + hydroquinone
-
Substrates: -
Products: -
?
D-xylose + 1,4-benzoquinone
2-dehydro-D-xylose + hydroquinone
Substrates: -
Products: -
?
D-xylose + acceptor
?
-
Substrates: -
Products: -
?
D-xylose + acceptor
?
Substrates: relative activity towards D-glucose (100%): 113%
Products: -
?
D-xylose + ferricenium ion
? + ferrocene
Substrates: -
Products: -
?
D-xylose + ferricenium ion
? + ferrocene
Substrates: -
Products: -
?
D-xylose + ferricenium ion
? + ferrocene
Substrates: -
Products: -
?
D-xylose + ferrocenium ion
2-dehydro-D-xylose + ferrocene
Substrates: -
Products: -
?
D-xylose + ferrocenium ion
2-dehydro-D-xylose + ferrocene
Substrates: -
Products: -
?
D-xylose + ferrocenium ion
2-dehydro-D-xylose + ferrocene
Substrates: -
Products: -
?
erlose + 1,4-benzoquinone
3-dehydroerlose + hydroquinone
-
Substrates: quinone-dependent PDH oxidizes erlose at exclusively C-3 of its terminal glucopyranosyl moiety, 9% of the activity with D-glucose
Products: -
?
erlose + 1,4-benzoquinone
3-dehydroerlose + hydroquinone
-
Substrates: quinone-dependent PDH oxidizes erlose at exclusively C-3 of its terminal glucopyranosyl moiety, 9% of the activity with D-glucose
Products: -
?
L-arabinose + acceptor
?
-
Substrates: -
Products: -
?
L-arabinose + acceptor
?
Substrates: relative activity towards D-glucose (100%): 100%
Products: -
?
L-arabinose + ferrocenium ion
2-dehydro-L-arabinose + ferrocene
Substrates: -
Products: -
?
L-arabinose + ferrocenium ion
2-dehydro-L-arabinose + ferrocene
Substrates: -
Products: -
?
L-arabinose + ferrocenium ion
2-dehydro-L-arabinose + ferrocene
Substrates: -
Products: -
?
L-fucose + acceptor
?
-
Substrates: -
Products: -
?
L-fucose + acceptor
?
Substrates: relative activity towards D-glucose (100%): 7%
Products: -
?
lactose + acceptor
lactobiono-1,5-lactone + 2-dehydrolactose + 2,3-didehydrolactose + reduced acceptor
Q3L1D2
Substrates: -
Products: -
?
lactose + acceptor
lactobiono-1,5-lactone + 2-dehydrolactose + 2,3-didehydrolactose + reduced acceptor
-
Substrates: -
Products: -
?
lactose + acceptor
lactobiono-1,5-lactone + 2-dehydrolactose + 2,3-didehydrolactose + reduced acceptor
Substrates: -
Products: -
?
lactose + ferricenium ion
? + ferrocene
Substrates: -
Products: -
?
lactose + ferricenium ion
? + ferrocene
Substrates: -
Products: -
?
lactose + ferrocenium ion
? + ferrocene
Substrates: 48% of the lactose is converted and yields 88% lactobionic acid and 12% 2-dehydrolactose
Products: -
?
lactose + ferrocenium ion
? + ferrocene
Substrates: the enzyme converts lactose to nearly 100%, producing 69% lactobionic acid and 31% 2-dehydrolactose
Products: -
?
lactose + ferrocenium ion
? + ferrocene
Substrates: -
Products: -
?
lactose + ferrocenium ion
? + ferrocene
-
Substrates: -
Products: -
?
maltose + 1,4-benzoquinone
?
-
Substrates: 93% of the activity with D-glucose
Products: -
?
maltose + 1,4-benzoquinone
?
-
Substrates: 64% of the activity with D-glucose
Products: -
?
maltotriose + 1,4-benzoquinone
?
-
Substrates: 70% of the activity with D-glucose
Products: -
?
maltotriose + 1,4-benzoquinone
?
-
Substrates: 21% of the activity with D-glucose
Products: -
?
maltotriose + ferricenium ion
?
-
Substrates: 14% of the activity with D-glucose
Products: -
?
maltotriose + ferricenium ion
?
-
Substrates: -
Products: -
r
maltotriose + ferricenium ion
? + ferrocene
Substrates: -
Products: -
?
maltotriose + ferricenium ion
? + ferrocene
Substrates: -
Products: -
?
melizitose + 1,4-benzoquinone
3-dehydromelizitose + hydroquinone
-
Substrates: quinone-dependent PDH oxidizes melezitose exclusively at C-3 of its terminal glucopyranosyl moiety, 7% of the activity with D-glucose
Products: -
?
melizitose + 1,4-benzoquinone
3-dehydromelizitose + hydroquinone
-
Substrates: quinone-dependent PDH oxidizes melezitose exclusively at C-3 of its terminal glucopyranosyl moiety, 7% of the activity with D-glucose
Products: -
?
methyl-alpha-D-galactopyranoside + 1,4-benzoquinone
methyl-alpha-3-dehydro-D-galactopyranoside + hydroquinone
-
Substrates: 23% of the activity with D-glucose
Products: -
?
methyl-alpha-D-galactopyranoside + 1,4-benzoquinone
methyl-alpha-3-dehydro-D-galactopyranoside + hydroquinone
-
Substrates: formation of the C-3 carbonyl derivative, 7% of the activity with D-glucose
Products: -
?
methyl-alpha-D-glucopyranoside + 1,4-benzoquinone
methyl-alpha-3-dehydro-D-glucopyranoside + hydroquinone
-
Substrates: 117% of the activity with D-glucose
Products: -
?
methyl-alpha-D-glucopyranoside + 1,4-benzoquinone
methyl-alpha-3-dehydro-D-glucopyranoside + hydroquinone
-
Substrates: formation of the C-3 carbonyl derivative, 126% of the activity with D-glucose
Products: -
?
methyl-beta-D-glucopyranoside + 1,4-benzoquinone
methyl-beta-3-dehydro-D-glucopyranoside + hydroquinone
-
Substrates: 69% of the activity with D-glucose
Products: -
?
methyl-beta-D-glucopyranoside + 1,4-benzoquinone
methyl-beta-3-dehydro-D-glucopyranoside + hydroquinone
-
Substrates: formation of the C-3 carbonyl derivative, 20% of the activity with D-glucose
Products: -
?
pyranose + acceptor
2,3-didehydropyranose + reduced acceptor
Substrates: -
Products: -
?
pyranose + acceptor
2,3-didehydropyranose + reduced acceptor
Q3L1D2
Substrates: -
Products: -
?
pyranose + acceptor
2,3-didehydropyranose + reduced acceptor
-
Substrates: -
Products: -
?
pyranose + acceptor
2,3-didehydropyranose + reduced acceptor
Substrates: -
Products: -
?
pyranose + acceptor
2,3-didehydropyranose + reduced acceptor
Substrates: -
Products: -
?
pyranose + acceptor
2-dehydropyranose (or 3-dehydropyranose or 2,3-didehydropyranose) + reduced acceptor
-
Substrates: -
Products: -
?
pyranose + acceptor
2-dehydropyranose (or 3-dehydropyranose or 2,3-didehydropyranose) + reduced acceptor
-
Substrates: -
Products: -
?
pyranose + acceptor
2-dehydropyranose (or 3-dehydropyranose or 2,3-didehydropyranose) + reduced acceptor
-
Substrates: -
Products: -
?
pyranose + acceptor
2-dehydropyranose (or 3-dehydropyranose or 2,3-didehydropyranose) + reduced acceptor
-
Substrates: -
Products: -
?
pyranose + acceptor
2-dehydropyranose + reduced acceptor
Substrates: -
Products: -
?
pyranose + acceptor
2-dehydropyranose + reduced acceptor
Q3L1D2
Substrates: -
Products: -
?
pyranose + acceptor
2-dehydropyranose + reduced acceptor
-
Substrates: -
Products: -
?
pyranose + acceptor
2-dehydropyranose + reduced acceptor
Substrates: -
Products: -
?
pyranose + acceptor
2-dehydropyranose + reduced acceptor
Substrates: -
Products: -
?
pyranose + acceptor
2-dehydropyranose + reduced acceptor
Substrates: -
Products: 2-dehydropyranose or 1-dehydropyranose or 3-dehydropyranose or 2,3-didehydropyranose
?
pyranose + acceptor
3-dehydropyranose + reduced acceptor
Substrates: -
Products: -
?
pyranose + acceptor
3-dehydropyranose + reduced acceptor
Q3L1D2
Substrates: -
Products: -
?
pyranose + acceptor
3-dehydropyranose + reduced acceptor
-
Substrates: -
Products: -
?
pyranose + acceptor
3-dehydropyranose + reduced acceptor
Substrates: -
Products: -
?
pyranose + acceptor
3-dehydropyranose + reduced acceptor
Substrates: -
Products: -
?
pyranoside + acceptor
3,4-didehydropyranoside + reduced acceptor
Q3L1D2
Substrates: -
Products: -
?
pyranoside + acceptor
3,4-didehydropyranoside + reduced acceptor
Substrates: -
Products: -
?
sucrose + 1,4-benzoquinone
?
-
Substrates: 63% of the activity with D-glucose
Products: -
?
sucrose + 1,4-benzoquinone
?
-
Substrates: 9% of the activity with D-glucose
Products: -
?
trehalose + 1,4-benzoquinone
?
-
Substrates: 54% of the activity with D-glucose
Products: -
?
trehalose + 1,4-benzoquinone
?
-
Substrates: 7% of the activity with D-glucose
Products: -
?
xylooligosaccharide + acceptor
?
Substrates: -
Products: -
?
xylooligosaccharide + acceptor
?
Substrates: activity of the PDH1 enzyme from Agaricus bisporus toward the tested xylooligosaccharides is higher than that measured for the PDH1 enzyme from Leucoagaricus meleagris. Activity values decrease with increasing length of the xylooligosaccharide and when using acidic rather than neutral substrates. The enzyme from Agaricus bisporus (AbPDH1) fully oxidizes all linear xylooligosaccharides, and 60-100% of all substituted xylooligosaccharides, after 24 h under the tested reaction conditions. Oxidation is restricted to the reducing and non-reducing xylopyranosyl residues
Products: -
?
additional information
?
-
-
Substrates: oxidative carbohydrate metabolism, pyranose 2-dehydrogenase may play an important role in fungal lignocellulose decomposition by interconnecting ligninolysis with degradation of cell wall polysaccharide components
Products: -
?
additional information
?
-
-
Substrates: enzyme is incapable of reducing O2 to H2O2, not: cytochrome c, NAD(P)+, nitroblue tetrazolium, low or no activity with D-altrose, D-fructose, D-lyxose, D-mannose, D-arabinitol, D-glucitol, D-mannitol or erythritol
Products: -
?
additional information
?
-
-
Substrates: with 1,4-benzoquinone as electron acceptor, PDH catalyzes the oxidation of numerous monosaccharides and oligosaccharides
Products: -
?
additional information
?
-
-
Substrates: with 1,4-benzoquinone as electron acceptor, PDH catalyzes the oxidation of numerous monosaccharides and oligosaccharides
Products: -
?
additional information
?
-
-
Substrates: broad selectivity for sugar substrates, O2 does not act as oxidant, not: D-allose, D-altrose, D-arabinose, D-ribose, D-fructose, L-sorbose, lactose, D-arabinitol, dulcitol, D-mannitol
Products: -
?
additional information
?
-
-
Substrates: extremely broad substrate tolerance
Products: -
?
additional information
?
-
-
Substrates: PDH can oxidise its substrate at the C-1, C-2 or C-3 as well as perform double oxidation at C-1,2, C-2,3 and it has no anomeric specificity. PDH does not show any detectable activity with oxygen
Products: -
?
additional information
?
-
Substrates: O2 is a poor electron acceptor
Products: -
?
additional information
?
-
-
Substrates: O2 is a poor electron acceptor
Products: -
?
additional information
?
-
-
Substrates: extremely broad substrate tolerance
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.0325 - 3.64
1,4-benzoquinone
0.079
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
with D-glucose as cosubstrate, in 100 mM sodium phosphate pH 7.5, at 30°C
0.0132 - 0.109
2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid
0.92
2,5-chloro-1,4-benzoquinone
-
at 30°C with 50 mM glucose as susbtrate
0.05
2,6-dichloroindophenol
Q3L1D2
at 30°C with 25 mM glucose as substrate
0.02 - 0.14
2,6-dichlorophenolindophenol
0.18
2-(hydroxymethyl)-6-methoxy-1,4-benzoquinone
Q3L1D2
at 30°C with 25 mM glucose as susbtrate
0.55 - 1.6
2-chloro-1,4-benzoquinone
12.14
2-deoxy-D-galactose
-
0.1 M ethanolamine buffer at pH 9.4
0.65
2-deoxy-D-glucose
-
0.1 M ethanolamine buffer at pH 9.4
0.12
2-methylcyclohexa-2,5-diene-1,4-dione
Q3L1D2
at 30°C with 25 mM glucose as susbtrate
0.02
3,5-di-t-butyl-1,2-benzoquinone
Q3L1D2
at 30°C with 25 mM glucose as susbtrate
0.22
3,5-di-tert-butyl-1,4-benzoquinone
-
at 30°C with 50 mM glucose as susbtrate
0.0017
3-ethylbenzthiazolin-6-sulfonic acid cation radical
Q3L1D2
at 30°C with 25 mM glucose as substrate
0.95
CuSO4
Q3L1D2
at 30°C with 25 mM glucose as susbtrate
17
D-arabinose
Q3L1D2
at 30°C with 0.4 mM ferricenium as electron acceptor
1.05
D-cellobiose
-
0.1 M ethanolamine buffer at pH 9.4
0.74
D-glucosamine
-
0.1 M ethanolamine buffer at pH 9.4
0.51
D-maltoheptaose
-
0.1 M ethanolamine buffer at pH 9.4
0.64
D-maltopentaose
-
0.1 M ethanolamine buffer at pH 9.4
137
D-sorbose
ferricenium ion as electron acceptor
0.05
D-trehalose
-
0.1 M ethanolamine buffer at pH 9.4
10.8
D-xylobiose
-
Fe3+ as electron acceptor
0.42
dimethylaminomethyl-ferricenium
Q3L1D2
at 30°C with 25 mM glucose as susbtrate
0.13
Fe3+
-
at 30°C with 50 mM glucose as susbtrate
0.12
ferricenium hexafluorophosphate
Q3L1D2
at 30°C with 25 mM glucose as susbtrate
0.0103 - 0.22
ferricenium ion
0.33
ferricyanide
Q3L1D2
at 30°C with 25 mM glucose as susbtrate
0.03 - 1.19
ferrocenium ion
17
gentiobiose
Q3L1D2
at 30°C with 0.4 mM ferricenium as electron acceptor
12.31
L-fucose
-
0.1 M ethanolamine buffer at pH 9.4
0.18
methyl-1,4-benzoquinone
-
at 30°C with 50 mM glucose as susbtrate
0.66 - 2
methyl-alpha-D-glucopyranoside
10.79
raffinose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C
0.35
sucrose
-
0.1 M ethanolamine buffer at pH 9.4
0.28
tetrachloro-1,4-benzoquinone
Q3L1D2
at 30°C with 25 mM glucose as susbtrate
0.39
tetrafluoro-1,4-benzoquinone
Q3L1D2
at 30°C with 25 mM glucose as susbtrate
0.0325
1,4-benzoquinone
isoform PDH2, with D-glucose as cosubstrate, at pH 4.0 and 30°C
0.12
1,4-benzoquinone
with D-glucose as cosubstrate, at pH 4.0 and 30°C
0.566
1,4-benzoquinone
with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30°C
1.03
1,4-benzoquinone
recombinant enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 2.0
1.1
1,4-benzoquinone
Q3L1D2
at 30°C with 25 mM glucose as susbtrate
1.38
1,4-benzoquinone
recombinant enzyme, at pH 4.0 and 30°C
1.38
1,4-benzoquinone
isoform PDH1, with D-glucose as cosubstrate, at pH 4.0 and 30°C
1.38
1,4-benzoquinone
wild type enzyme, with D-glucose as cosubstrate, at pH 4.0 and 30°C
1.82
1,4-benzoquinone
-
at 30°C with 50 mM glucose as susbtrate
1.82
1,4-benzoquinone
native enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 2.0
1.82
1,4-benzoquinone
native enzyme, at pH 3.0 and 30°C
1.88
1,4-benzoquinone
isoform PDH3, with D-glucose as cosubstrate, at pH 4.0 and 30°C
3.25
1,4-benzoquinone
with D-glucose as cosubstrate, at pH 4.0 and 30°C
3.64
1,4-benzoquinone
mutant enzyme H103Y, with D-glucose as cosubstrate, at pH 4.0 and 30°C
0.0132
2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid
isoform PDH3, with D-glucose as cosubstrate, at pH 4.0 and 30°C
0.0791
2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid
isoform PDH1, with D-glucose as cosubstrate, at pH 4.0 and 30°C
0.109
2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid
isoform PDH2, with D-glucose as cosubstrate, at pH 4.0 and 30°C
0.02
2,6-dichlorophenolindophenol
mutant enzyme H103Y, with D-glucose as cosubstrate, at pH 4.0 and 30°C
0.06
2,6-dichlorophenolindophenol
with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30°C
0.09
2,6-dichlorophenolindophenol
with D-glucose as cosubstrate, at pH 4.0 and 30°C
0.11
2,6-dichlorophenolindophenol
with D-glucose as cosubstrate, at pH 4.0 and 30°C
0.14
2,6-dichlorophenolindophenol
wild type enzyme, with D-glucose as cosubstrate, at pH 4.0 and 30°C
0.55
2-chloro-1,4-benzoquinone
-
at 30°C with 50 mM glucose as susbtrate
0.55
2-chloro-1,4-benzoquinone
native enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 5.0
0.55
2-chloro-1,4-benzoquinone
native enzyme, at pH 5.0 and 30°C
0.61
2-chloro-1,4-benzoquinone
recombinant enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 5.0
0.62
2-chloro-1,4-benzoquinone
recombinant enzyme, at pH 5.0 and 30°C
1.6
2-chloro-1,4-benzoquinone
Q3L1D2
at 30°C with 25 mM glucose as susbtrate
10.84
beta-lactose
-
0.1 M ethanolamine buffer at pH 9.4
120
beta-lactose
Q3L1D2
at 30°C with 0.4 mM ferricenium as electron acceptor
2.45
cellobiose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C
4.74
cellobiose
mutant enzyme N75G/N175Q, at pH 7.5 and 30°C
5.52
cellobiose
mutant enzyme N252Q, at pH 7.5 and 30°C
5.7
cellobiose
mutant enzyme N175Q/N252Q, at pH 7.5 and 30°C
5.72
cellobiose
mutant enzyme N75G/N175Q/N252Q, at pH 7.5 and 30°C
6.38
cellobiose
mutant enzyme N75G/N252Q, at pH 7.5 and 30°C
6.69
cellobiose
wild type enzyme, at pH 7.5 and 30°C
6.82
cellobiose
-
Fe3+ as electron acceptor
6.82
cellobiose
ferricenium ion as electron acceptor
6.82
cellobiose
with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication
7.1
cellobiose
mutant enzyme N75G, at pH 7.5 and 30°C
8.13
cellobiose
mutant enzyme N175Q, at pH 7.5 and 30°C
18.3
D-allose
isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
113
D-allose
isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
181
D-allose
isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
153
D-fructose
-
Fe3+ as electron acceptor
153
D-fructose
ferricenium ion as electron acceptor
153
D-fructose
with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication
0.64
D-galactose
isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
0.8
D-galactose
mutant enzyme N252Q, at pH 7.5 and 30°C
0.86
D-galactose
mutant enzyme N175Q/N252Q, at pH 7.5 and 30°C
1
D-galactose
wild type enzyme, at pH 7.5 and 30°C
1.02
D-galactose
recombinant enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5
1.05
D-galactose
-
Fe3+ as electron acceptor
1.05
D-galactose
ferricenium ion as electron acceptor
1.05
D-galactose
native enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5
1.05
D-galactose
using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C
1.05
D-galactose
with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication
1.07
D-galactose
using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C
1.07
D-galactose
wild type enzyme, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C
1.1
D-galactose
mutant enzyme N175Q, at pH 7.5 and 30°C
1.14
D-galactose
mutant enzyme N75G/N252Q, at pH 7.5 and 30°C
2.19
D-galactose
mutant enzyme N75G, at pH 7.5 and 30°C
3.09
D-galactose
mutant enzyme N75G/N175Q, at pH 7.5 and 30°C
3.55
D-galactose
mutant enzyme N75G/N175Q/N252Q, at pH 7.5 and 30°C
4.19
D-galactose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C
5.29
D-galactose
mutant enzyme H103Y, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C
7.13
D-galactose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
7.16
D-galactose
-
0.1 M ethanolamine buffer at pH 9.4
11
D-galactose
Q3L1D2
at 30°C with 0.4 mM ferricenium as electron acceptor
29.7
D-galactose
isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
29.8
D-galactose
-
25°C, ferricenium ion as electron acceptor
40.1
D-galactose
isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
74.99
D-galactose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
0.35
D-glucose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
0.44
D-glucose
Q3L1D2
at 0.1 M ethanolamine buffer at pH 9.4
0.49
D-glucose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
0.53
D-glucose
isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
0.58
D-glucose
mutant enzyme N252Q, at pH 7.5 and 30°C
0.65
D-glucose
-
0.1 M ethanolamine buffer at pH 9.4
0.69
D-glucose
using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C
0.69
D-glucose
wild type enzyme, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C
0.72
D-glucose
mutant enzyme N175Q/N252Q, at pH 7.5 and 30°C
0.78
D-glucose
Q3L1D2
at 30°C with 0.4 mM ferricenium as electron acceptor
0.82
D-glucose
-
Fe3+ as electron acceptor
0.82
D-glucose
ferricenium ion as electron acceptor
0.82
D-glucose
native enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5
0.82
D-glucose
using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C
0.82
D-glucose
with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication
0.82
D-glucose
isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
0.86
D-glucose
recombinant enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5
0.87
D-glucose
wild type enzyme, at pH 7.5 and 30°C
0.88
D-glucose
mutant enzyme N75G/N252Q, at pH 7.5 and 30°C
0.97
D-glucose
mutant enzyme N175Q, at pH 7.5 and 30°C
1.5
D-glucose
mutant enzyme N75G/N175Q, at pH 7.5 and 30°C
1.54
D-glucose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C
1.82
D-glucose
-
25°C, ferricenium ion as electron acceptor
1.85
D-glucose
mutant enzyme N75G, at pH 7.5 and 30°C
1.94
D-glucose
mutant enzyme N75G/N175Q/N252Q, at pH 7.5 and 30°C
3.28
D-glucose
isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
3.85
D-glucose
mutant enzyme H103Y, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C
0.34
D-maltose
-
0.1 M ethanolamine buffer at pH 9.4
8.61
D-maltose
-
Fe3+ as electron acceptor
8.61
D-maltose
ferricenium ion as electron acceptor
8.61
D-maltose
with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication
12
D-maltose
Q3L1D2
at 30°C with 0.4 mM ferricenium as electron acceptor
0.64
D-maltotriose
-
0.1 M ethanolamine buffer at pH 9.4
100
D-maltotriose
Q3L1D2
at 30°C with 0.4 mM ferricenium as electron acceptor
0.62
D-mannose
-
0.1 M ethanolamine buffer at pH 9.4
34.9
D-mannose
isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
74.2
D-mannose
wild type enzyme, at pH 7.5 and 30°C
83.7
D-mannose
mutant enzyme N252Q, at pH 7.5 and 30°C
87.9
D-mannose
mutant enzyme N175Q/N252Q, at pH 7.5 and 30°C
94
D-mannose
isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
108
D-mannose
-
Fe3+ as electron acceptor
108
D-mannose
ferricenium ion as electron acceptor
108
D-mannose
with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication
109.1
D-mannose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C
116
D-mannose
mutant enzyme N75G/N252Q, at pH 7.5 and 30°C
142
D-mannose
mutant enzyme N175Q, at pH 7.5 and 30°C
185
D-mannose
isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
227
D-mannose
mutant enzyme N75G, at pH 7.5 and 30°C
355
D-mannose
mutant enzyme N75G/N175Q, at pH 7.5 and 30°C
380
D-mannose
mutant enzyme N75G/N175Q/N252Q, at pH 7.5 and 30°C
59.7
D-ribose
-
Fe3+ as electron acceptor
59.7
D-ribose
ferricenium ion as electron acceptor
59.7
D-ribose
with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication
79.1
D-talose
-
Fe3+ as electron acceptor
79.1
D-talose
ferricenium ion as electron acceptor
79.1
D-talose
with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication
1.03
D-xylose
recombinant enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5
1.44
D-xylose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
1.92
D-xylose
using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C
1.93
D-xylose
-
Fe3+ as electron acceptor
1.93
D-xylose
ferricenium ion as electron acceptor
1.93
D-xylose
native enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5
1.93
D-xylose
using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C
1.93
D-xylose
with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication
2.39
D-xylose
isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
4.19
D-xylose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
4.75
D-xylose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C
5.84
D-xylose
-
0.1 M ethanolamine buffer at pH 9.4
9.34
D-xylose
isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
17
D-xylose
isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
25
D-xylose
Q3L1D2
at 30°C with 0.4 mM ferricenium as electron acceptor
29.2
D-xylose
-
25°C, ferricenium ion as electron acceptor
0.0103
ferricenium ion
isoform PDH3, with D-glucose as cosubstrate, at pH 4.0 and 30°C
0.0201
ferricenium ion
isoform PDH2, at pH 8.5 and 30°C
0.13
ferricenium ion
native enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 8.5
0.161
ferricenium ion
isoform PDH1, with D-glucose as cosubstrate, at pH 4.0 and 30°C
0.22
ferricenium ion
recombinant enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 8.5
0.03
ferrocenium ion
with D-glucose as cosubstrate, at pH 8.5 and 30°C
0.1
ferrocenium ion
mutant enzyme H103Y, with D-glucose as cosubstrate, at pH 8.5 and 30°C
0.1
ferrocenium ion
mutant enzyme N75G/N175Q/N252Q, at pH 7.5 and 30°C
0.11
ferrocenium ion
mutant enzyme N75G/N175Q, at pH 7.5 and 30°C
0.13
ferrocenium ion
native enzyme, at pH 8.5 and 30°C
0.16
ferrocenium ion
recombinant enzyme, at pH 8.5 and 30°C
0.16
ferrocenium ion
wild type enzyme, with D-glucose as cosubstrate, at pH 8.5 and 30°C
0.17
ferrocenium ion
with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30°C
0.24
ferrocenium ion
mutant enzyme N75G, at pH 7.5 and 30°C
0.27
ferrocenium ion
wild type enzyme, at pH 7.5 and 30°C
0.29
ferrocenium ion
mutant enzyme N75G/N252Q, at pH 7.5 and 30°C
0.3
ferrocenium ion
mutant enzyme N252Q, at pH 7.5 and 30°C
0.37
ferrocenium ion
mutant enzyme N175Q/N252Q, at pH 7.5 and 30°C
0.39
ferrocenium ion
mutant enzyme N75Q, at pH 7.5 and 30°C
1.19
ferrocenium ion
with D-glucose as cosubstrate, at pH 8.5 and 30°C
0.44
L-arabinose
native enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5
0.49
L-arabinose
using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C
0.54
L-arabinose
-
Fe3+ as electron acceptor
0.54
L-arabinose
ferricenium ion as electron acceptor
0.54
L-arabinose
using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C
0.54
L-arabinose
with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication
0.74
L-arabinose
recombinant enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5
1.33
L-arabinose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C
4.16
L-arabinose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
4.23
L-arabinose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
8.63
L-arabinose
-
0.1 M ethanolamine buffer at pH 9.4
24
L-arabinose
-
25°C, ferricenium ion as electron acceptor
137
L-sorbose
-
Fe3+ as electron acceptor
137
L-sorbose
with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication
40.51
lactose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C
53.16
lactose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
76.7
lactose
isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
113.9
lactose
recombinant enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5
128
lactose
using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C
128
lactose
wild type enzyme, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C
134
lactose
-
Fe3+ as electron acceptor
134
lactose
ferricenium ion as electron acceptor
134
lactose
native enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5
134
lactose
using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C
134
lactose
with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication
235
lactose
isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
293.8
lactose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
395.9
lactose
mutant enzyme H103Y, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C
2288
lactose
isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
2.16
maltose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C
2.22
maltose
isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
10.3
maltose
isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
11.6
maltose
isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
54.6
maltotriose
isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
62.5
maltotriose
isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
154
maltotriose
isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
156
maltotriose
-
Fe3+ as electron acceptor
156
maltotriose
ferricenium ion as electron acceptor
156
maltotriose
with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication
0.66
methyl-alpha-D-glucopyranoside
isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
0.95
methyl-alpha-D-glucopyranoside
isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
2
methyl-alpha-D-glucopyranoside
isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
0.095
O2
wild type enzyme, with D-glucose as cosubstrate, at pH 7.4 and 30°C
0.5
O2
mutant enzyme H103Y, with D-glucose as cosubstrate, at pH 7.4 and 30°C
10.8
xylobiose
ferricenium ion as electron acceptor
10.8
xylobiose
with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
3.57 - 76
1,4-benzoquinone
101.2
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
with D-glucose as cosubstrate, in 100 mM sodium phosphate pH 7.5, at 30°C
4.28 - 7.57
2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid
2.8 - 57.6
2,6-dichlorophenolindophenol
12.9 - 15.1
2-chloro-1,4-benzoquinone
15.7
D-sorbose
ferricenium ion as electron acceptor
9.86 - 203
ferricenium ion
19.92 - 130
ferrocenium ion
15.7
L-sorbose
with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication
1 - 85
methyl-alpha-D-glucopyranoside
19.61
raffinose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C
3.57
1,4-benzoquinone
isoform PDH3, with D-glucose as cosubstrate, at pH 4.0 and 30°C
12.89
1,4-benzoquinone
with D-glucose as cosubstrate, at pH 4.0 and 30°C
13.6
1,4-benzoquinone
mutant enzyme H103Y, with D-glucose as cosubstrate, at pH 4.0 and 30°C
23.4
1,4-benzoquinone
isoform PDH2, with D-glucose as cosubstrate, at pH 4.0 and 30°C
34.82
1,4-benzoquinone
with D-glucose as cosubstrate, at pH 4.0 and 30°C
44.2
1,4-benzoquinone
with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30°C
59
1,4-benzoquinone
recombinant enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 2.0
65.4
1,4-benzoquinone
recombinant enzyme, at pH 4.0 and 30°C
65.4
1,4-benzoquinone
isoform PDH1, with D-glucose as cosubstrate, at pH 4.0 and 30°C
65.4
1,4-benzoquinone
wild type enzyme, with D-glucose as cosubstrate, at pH 4.0 and 30°C
76
1,4-benzoquinone
native enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 2.0
76
1,4-benzoquinone
native enzyme, at pH 3.0 and 30°C
4.28
2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid
isoform PDH2, with D-glucose as cosubstrate, at pH 4.0 and 30°C
5.32
2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid
isoform PDH3, with D-glucose as cosubstrate, at pH 4.0 and 30°C
7.57
2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid
isoform PDH1, with D-glucose as cosubstrate, at pH 4.0 and 30°C
2.8
2,6-dichlorophenolindophenol
mutant enzyme H103Y, with D-glucose as cosubstrate, at pH 4.0 and 30°C
7.65
2,6-dichlorophenolindophenol
with D-glucose as cosubstrate, at pH 4.0 and 30°C
10.56
2,6-dichlorophenolindophenol
with D-glucose as cosubstrate, at pH 4.0 and 30°C
40.7
2,6-dichlorophenolindophenol
wild type enzyme, with D-glucose as cosubstrate, at pH 4.0 and 30°C
57.6
2,6-dichlorophenolindophenol
with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30°C
12.9
2-chloro-1,4-benzoquinone
recombinant enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 5.0
12.9
2-chloro-1,4-benzoquinone
recombinant enzyme, at pH 5.0 and 30°C
15.1
2-chloro-1,4-benzoquinone
native enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 5.0
15.1
2-chloro-1,4-benzoquinone
native enzyme, at pH 5.0 and 30°C
25.63
cellobiose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C
29.1
cellobiose
mutant enzyme N75G/N175Q, at pH 7.5 and 30°C
30.9
cellobiose
wild type enzyme, at pH 7.5 and 30°C
31.3
cellobiose
mutant enzyme N75G/N252Q, at pH 7.5 and 30°C
33
cellobiose
mutant enzyme N75G, at pH 7.5 and 30°C
34.7
cellobiose
mutant enzyme N75G/N175Q/N252Q, at pH 7.5 and 30°C
35.2
cellobiose
mutant enzyme N175Q/N252Q, at pH 7.5 and 30°C
38.4
cellobiose
ferricenium ion as electron acceptor
38.4
cellobiose
with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication
43.8
cellobiose
mutant enzyme N252Q, at pH 7.5 and 30°C
55.1
cellobiose
mutant enzyme N175Q, at pH 7.5 and 30°C
18.4
D-allose
isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
35.3
D-allose
isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
40.2
D-allose
isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
16.5
D-fructose
ferricenium ion as electron acceptor
16.5
D-fructose
with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication
5.23
D-galactose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
19.3
D-galactose
isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
24.77
D-galactose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
25.7
D-galactose
mutant enzyme N75G, at pH 7.5 and 30°C
28.7
D-galactose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C
28.9
D-galactose
mutant enzyme H103Y, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C
31.8
D-galactose
wild type enzyme, at pH 7.5 and 30°C
34.2
D-galactose
mutant enzyme N75G/N175Q, at pH 7.5 and 30°C
34.3
D-galactose
isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
37.9
D-galactose
mutant enzyme N75G/N252Q, at pH 7.5 and 30°C
38.9
D-galactose
mutant enzyme N75G/N175Q/N252Q, at pH 7.5 and 30°C
39.3
D-galactose
mutant enzyme N175Q/N252Q, at pH 7.5 and 30°C
40.3
D-galactose
mutant enzyme N175Q, at pH 7.5 and 30°C
45.9
D-galactose
mutant enzyme N252Q, at pH 7.5 and 30°C
46.6
D-galactose
recombinant enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5
47.3
D-galactose
using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C
47.3
D-galactose
wild type enzyme, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C
48.5
D-galactose
ferricenium ion as electron acceptor
48.5
D-galactose
native enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5
48.5
D-galactose
using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C
48.5
D-galactose
with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication
137
D-galactose
isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
3 - 6
D-glucose
mutant enzyme N75G/N175Q, at pH 7.5 and 30°C
4.1
D-glucose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
8.16
D-glucose
isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
13.02
D-glucose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
27.4
D-glucose
mutant enzyme H103Y, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C
33.7
D-glucose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C
35.7
D-glucose
isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
36.6
D-glucose
wild type enzyme, at pH 7.5 and 30°C
37.8
D-glucose
using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C
37.8
D-glucose
wild type enzyme, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C
40.8
D-glucose
mutant enzyme N75G/N252Q, at pH 7.5 and 30°C
42.2
D-glucose
isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
45.3
D-glucose
mutant enzyme N75G, at pH 7.5 and 30°C
45.4
D-glucose
mutant enzyme N175Q/N252Q, at pH 7.5 and 30°C
45.9
D-glucose
ferricenium ion as electron acceptor
45.9
D-glucose
native enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5
45.9
D-glucose
using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C
45.9
D-glucose
with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication
48.06
D-glucose
recombinant enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5
48.1
D-glucose
mutant enzyme N175Q, at pH 7.5 and 30°C
49.7
D-glucose
mutant enzyme N75G/N175Q/N252Q, at pH 7.5 and 30°C
50.8
D-glucose
mutant enzyme N252Q, at pH 7.5 and 30°C
43.2
D-maltose
ferricenium ion as electron acceptor
43.2
D-maltose
with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication
13.31
D-mannose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C
22.1
D-mannose
mutant enzyme N75G, at pH 7.5 and 30°C
26.5
D-mannose
isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
27
D-mannose
mutant enzyme N75G/N175Q/N252Q, at pH 7.5 and 30°C
27.4
D-mannose
wild type enzyme, at pH 7.5 and 30°C
29.3
D-mannose
ferricenium ion as electron acceptor
29.3
D-mannose
with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication
31.5
D-mannose
mutant enzyme N75G/N252Q, at pH 7.5 and 30°C
33.5
D-mannose
mutant enzyme N175Q/N252Q, at pH 7.5 and 30°C
34.7
D-mannose
isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
35
D-mannose
mutant enzyme N75G/N175Q, at pH 7.5 and 30°C
40.8
D-mannose
mutant enzyme N175Q, at pH 7.5 and 30°C
41.9
D-mannose
mutant enzyme N252Q, at pH 7.5 and 30°C
109
D-mannose
isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
31.4
D-ribose
ferricenium ion as electron acceptor
31.4
D-ribose
with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication
21.3
D-talose
ferricenium ion as electron acceptor
21.3
D-talose
with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication
6.37
D-xylose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
29.16
D-xylose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
29.5
D-xylose
isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
36.9
D-xylose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C
41.5
D-xylose
recombinant enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5
43.4
D-xylose
ferricenium ion as electron acceptor
43.4
D-xylose
native enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5
43.4
D-xylose
using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C
43.4
D-xylose
with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication
51.3
D-xylose
isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
62.4
D-xylose
using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C
146
D-xylose
isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
9.86
ferricenium ion
isoform PDH3, with D-glucose as cosubstrate, at pH 4.0 and 30°C
48.1
ferricenium ion
isoform PDH2, at pH 8.5 and 30°C
104
ferricenium ion
native enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 8.5
130
ferricenium ion
isoform PDH1, with D-glucose as cosubstrate, at pH 4.0 and 30°C
203
ferricenium ion
recombinant enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 8.5
19.92
ferrocenium ion
with D-glucose as cosubstrate, at pH 8.5 and 30°C
22.07
ferrocenium ion
with D-glucose as cosubstrate, at pH 8.5 and 30°C
26
ferrocenium ion
mutant enzyme H103Y, with D-glucose as cosubstrate, at pH 8.5 and 30°C
44.8
ferrocenium ion
mutant enzyme N75G/N175Q, at pH 7.5 and 30°C
50.7
ferrocenium ion
mutant enzyme N175Q/N252Q, at pH 7.5 and 30°C
54.2
ferrocenium ion
mutant enzyme N75G/N175Q/N252Q, at pH 7.5 and 30°C
54.6
ferrocenium ion
mutant enzyme N75G/N252Q, at pH 7.5 and 30°C
65.8
ferrocenium ion
mutant enzyme N75G, at pH 7.5 and 30°C
66.3
ferrocenium ion
mutant enzyme N252Q, at pH 7.5 and 30°C
87.4
ferrocenium ion
wild type enzyme, at pH 7.5 and 30°C
104
ferrocenium ion
native enzyme, at pH 8.5 and 30°C
111.1
ferrocenium ion
with D-glucose as cosubstrate, in 100 mM sodium acetate pH 4.0, at 30°C
125
ferrocenium ion
mutant enzyme N75Q, at pH 7.5 and 30°C
130
ferrocenium ion
recombinant enzyme, at pH 8.5 and 30°C
130
ferrocenium ion
wild type enzyme, with D-glucose as cosubstrate, at pH 8.5 and 30°C
3.05
L-arabinose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
22.9
L-arabinose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
29.6
L-arabinose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C
34.7
L-arabinose
using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C
37.2
L-arabinose
ferricenium ion as electron acceptor
37.2
L-arabinose
native enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5
37.2
L-arabinose
using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C
37.2
L-arabinose
with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication
45.7
L-arabinose
recombinant enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5
3.12
lactose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
15.95
lactose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C
24.65
lactose
with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
24.7
lactose
mutant enzyme H103Y, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C
31.9
lactose
recombinant enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5
33.7
lactose
isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
39.6
lactose
ferricenium ion as electron acceptor
39.6
lactose
native enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5
39.6
lactose
using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C
39.6
lactose
with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication
41
lactose
using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C
41
lactose
wild type enzyme, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C
75.3
lactose
isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
209
lactose
isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
0.48
maltose
isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
7.63
maltose
isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
27.26
maltose
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C
44.7
maltose
isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
16.3
maltotriose
isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
21.2
maltotriose
isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
23.6
maltotriose
ferricenium ion as electron acceptor
23.6
maltotriose
with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication
35.9
maltotriose
isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
1
methyl-alpha-D-glucopyranoside
isoform PDH3, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
41.5
methyl-alpha-D-glucopyranoside
isoform PDH1, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
85
methyl-alpha-D-glucopyranoside
isoform PDH2, with ferrocenium ion as cosubstrate, at pH 7.5 and 30°C
50.4
xylobiose
ferricenium ion as electron acceptor
50.4
xylobiose
with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication