General Stability | Organism |
---|---|
repeated freezing and thawing, two cycles, in the absence of glycerol has no effect on PDH activity | Chlorophyllum rhacodes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.82 | - |
D-glucose | 25°C, ferricenium ion as electron acceptor | Chlorophyllum rhacodes | |
24 | - |
L-arabinose | 25°C, ferricenium ion as electron acceptor | Chlorophyllum rhacodes | |
29.2 | - |
D-xylose | 25°C, ferricenium ion as electron acceptor | Chlorophyllum rhacodes | |
29.8 | - |
D-galactose | 25°C, ferricenium ion as electron acceptor | Chlorophyllum rhacodes |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | active extracellular secretion of PDH | Chlorophyllum rhacodes | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
76000 | - |
gel filtration | Chlorophyllum rhacodes |
78000 | - |
1 * 78000, SDS-PAGE | Chlorophyllum rhacodes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlorophyllum rhacodes | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | heavily glycosylated, the sugar moiety of PDH comprises 17.3% of its total molecular mass | Chlorophyllum rhacodes |
Purification (Comment) | Organism |
---|---|
46fold | Chlorophyllum rhacodes |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Chlorophyllum rhacodes | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
67 | - |
25°C, D-glucose and ferricenium ion as substrates | Chlorophyllum rhacodes |
Storage Stability | Organism |
---|---|
4°C, purified PDH, 20 mM sodium phosphate buffer, pH 7, 0.02% ProClin 300, 2 months, no loss of activity | Chlorophyllum rhacodes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-D-glucose + 1,4-benzoquinone | 49% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
2-deoxy-D-glucose + ferricenium ion | 21% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
3-dehydro-D-glucose + 1,4-benzoquinone | - |
Chlorophyllum rhacodes | 2,3-didehydro-D-glucose + hydroquinone | - |
? | |
a pyranoside + acceptor | - |
Chlorophyllum rhacodes | a 3-dehydropyranoside (or 3,4-didehydropyranoside) + reduced acceptor | - |
? | |
cellobiose + 1,4-benzoquinone | 13% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
cellobiose + ferricenium ion | 7% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
D-galactose + 1,4-benzoquinone | 66% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
D-galactose + ferricenium ion | 60% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
D-glucono-1,5-lactone + 1,4-benzoquinone | 20% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
D-glucono-1,5-lactone + ferricenium ion | 8% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
D-glucose + 1,4-benzoquinone | oxidizes D-glucose exclusively at C-3, D-glucose is the preferred electron donor susbtrate | Chlorophyllum rhacodes | 3-dehydro-D-glucose + hydroquinone | - |
? | |
D-glucose + ferricenium ion | oxidizes D-glucose exclusively at C-3, D-glucose is the preferred electron donor susbtrate | Chlorophyllum rhacodes | 3-dehydro-D-glucose + ferrocene | - |
? | |
D-lyxose + 1,4-benzoquinone | 5% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
D-lyxose + ferricenium ion | 5% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
D-mannose + 1,4-benzoquinone | 32% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
D-mannose + ferricenium ion | 26% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
D-xylose + 1,4-benzoquinone | 72% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
D-xylose + ferricenium ion | 81% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
L-arabinose + 1,4-benzoquinone | 54% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
L-arabinose + ferricenium ion | 72% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
L-glucose + ferricenium ion | 11% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
maltose + 1,4-benzoquinone | 64% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
maltose + ferricenium ion | 45% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
maltotriose + 1,4-benzoquinone | 21% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
maltotriose + ferricenium ion | 14% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
methyl-alpha-D-galactopyranoside + 1,4-benzoquinone | 11% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
methyl-alpha-D-galactopyranoside + ferricenium ion | 8% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
methyl-alpha-D-glucopyranoside + 1,4-benzoquinone | 141% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
methyl-alpha-D-glucopyranoside + ferricenium ion | 143% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
methyl-beta-D-glucopyranoside + 1,4-benzoquinone | 30% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
methyl-beta-D-glucopyranoside + ferricenium ion | 17% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
additional information | broad selectivity for sugar substrates, O2 does not act as oxidant, not: D-allose, D-altrose, D-arabinose, D-ribose, D-fructose, L-sorbose, lactose, D-arabinitol, dulcitol, D-mannitol | Chlorophyllum rhacodes | ? | - |
? | |
pyranose + acceptor | - |
Chlorophyllum rhacodes | 2-dehydropyranose (or 3-dehydropyranose or 2,3-didehydropyranose) + reduced acceptor | - |
? | |
sucrose + 1,4-benzoquinone | 9% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
sucrose + ferricenium ion | 6% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
trehalose + 1,4-benzoquinone | 7% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? | |
trehalose + ferricenium ion | 8% of the activity with D-glucose | Chlorophyllum rhacodes | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 78000, SDS-PAGE | Chlorophyllum rhacodes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
ferricenium ion as electron acceptor | Chlorophyllum rhacodes |
75 | - |
1,4-benzoquinone as electron acceptor | Chlorophyllum rhacodes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
50 mM sodium citrate buffer, 1,4-benzoquinone as electron acceptor | Chlorophyllum rhacodes |
9.5 | - |
50 mM glycine buffer, ferricenium ion as electron acceptor | Chlorophyllum rhacodes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | functions as the cofactor | Chlorophyllum rhacodes | |
flavin | flavoprotein, FAD as cofactor | Chlorophyllum rhacodes |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Chlorophyllum rhacodes | isoelectric focusing | - |
4.2 |