BRENDA - Enzyme Database
show all sequences of 1.1.3.44

Elaboration of neosamine rings in the biosynthesis of neomycin and butirosin

Huang, F.; Spiteller, D.; Koorbanally, N.A.; Li, Y.; Llewellyn, N.M.; Spencer, J.B.; ChemBioChem 8, 283-288 (2007)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Streptomyces fradiae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60034
-
x * 60034, LC-ESI-MS
Streptomyces fradiae
Organism
Organism
UniProt
Commentary
Textmining
Streptomyces fradiae
Q53U15
-
-
Streptomyces fradiae NCIMB 8233
Q53U15
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
6'''-deamino-6'''-hydroxyneomycin C + O2
oxygen-dependent mechanism for the regeneration of the FAD cofactor
719294
Streptomyces fradiae
6'''-deamino-6'''-oxoneomycin C + H2O2
-
-
-
?
6'''-deamino-6'''-hydroxyneomycin C + O2
oxygen-dependent mechanism for the regeneration of the FAD cofactor
719294
Streptomyces fradiae NCIMB 8233
6'''-deamino-6'''-oxoneomycin C + H2O2
-
-
-
?
additional information
enzyme also catalyzes reaction of EC 1.1.3.43, paromamine 6'-oxidase
719294
Streptomyces fradiae
?
-
-
-
?
additional information
enzyme also catalyzes reaction of EC 1.1.3.43, paromamine 6'-oxidase
719294
Streptomyces fradiae NCIMB 8233
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 60034, LC-ESI-MS
Streptomyces fradiae
Synonyms
Synonyms
Commentary
Organism
Neo-11
-
Streptomyces fradiae
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Streptomyces fradiae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Streptomyces fradiae
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
-
Streptomyces fradiae
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Streptomyces fradiae
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
-
Streptomyces fradiae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60034
-
x * 60034, LC-ESI-MS
Streptomyces fradiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
6'''-deamino-6'''-hydroxyneomycin C + O2
oxygen-dependent mechanism for the regeneration of the FAD cofactor
719294
Streptomyces fradiae
6'''-deamino-6'''-oxoneomycin C + H2O2
-
-
-
?
6'''-deamino-6'''-hydroxyneomycin C + O2
oxygen-dependent mechanism for the regeneration of the FAD cofactor
719294
Streptomyces fradiae NCIMB 8233
6'''-deamino-6'''-oxoneomycin C + H2O2
-
-
-
?
additional information
enzyme also catalyzes reaction of EC 1.1.3.43, paromamine 6'-oxidase
719294
Streptomyces fradiae
?
-
-
-
?
additional information
enzyme also catalyzes reaction of EC 1.1.3.43, paromamine 6'-oxidase
719294
Streptomyces fradiae NCIMB 8233
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 60034, LC-ESI-MS
Streptomyces fradiae
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Streptomyces fradiae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Streptomyces fradiae
General Information
General Information
Commentary
Organism
physiological function
joint activity of enzyme and 6'-oxoglucosaminyl:L-glutamate aminotransferase Neo-18 is responsible for the conversion of paromamine to neaminein the biosynthetic pathway of neomycin through a mechanism of FAD-dependent dehydrogenation followed by a pyridoxal-5'-phosphate-mediated transamination
Streptomyces fradiae
General Information (protein specific)
General Information
Commentary
Organism
physiological function
joint activity of enzyme and 6'-oxoglucosaminyl:L-glutamate aminotransferase Neo-18 is responsible for the conversion of paromamine to neaminein the biosynthetic pathway of neomycin through a mechanism of FAD-dependent dehydrogenation followed by a pyridoxal-5'-phosphate-mediated transamination
Streptomyces fradiae
Other publictions for EC 1.1.3.44
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
719676
Clausnitzer
The oxidoreductases LivQ and N ...
Streptomyces lividus, Streptomyces fradiae, Streptomyces fradiae NCIMB 8233
J. Appl. Microbiol.
111
642-651
2011
-
1
2
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
2
-
7
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
719294
Huang
Elaboration of neosamine rings ...
Streptomyces fradiae, Streptomyces fradiae NCIMB 8233
ChemBioChem
8
283-288
2007
-
-
1
-
-
-
-
-
-
-
1
-
-
6
-
-
-
-
-
-
-
-
4
1
1
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
4
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-