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EC Tree
IUBMB Comments Contains FAD. Involved in the biosynthetic pathway of aminoglycoside antibiotics of the neomycin family. Works in combination with EC 2.6.1.95, neomycin C transaminase, to replace the 6'''-hydroxy group of 6'''-hydroxyneomycin C with an amino group.
Also catalyses EC 1.1.3.43, paromamine 6'-oxidase.
The expected taxonomic range for this enzyme is: Streptomyces
Synonyms
neo-11,
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Neo-11
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neoQ
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obsolete gene name
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6'''-deamino-6'''-hydroxyneomycin C + O2 = 6'''-deamino-6'''-oxoneomycin C + H2O2
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6'''-deamino-6'''-hydroxyneomycin C:oxygen 6'''-oxidoreductase
Contains FAD. Involved in the biosynthetic pathway of aminoglycoside antibiotics of the neomycin family. Works in combination with EC 2.6.1.95, neomycin C transaminase, to replace the 6'''-hydroxy group of 6'''-hydroxyneomycin C with an amino group.
Also catalyses EC 1.1.3.43, paromamine 6'-oxidase.
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2'''-N-acetyl-6'''-hydroxyneomycin C + O2
2'''-N-acetyl-6'''-oxoneomycin C + H2O2
6'''-deamino-6'''-hydroxyneomycin C + O2
6'''-deamino-6'''-oxoneomycin C + H2O2
additional information
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2'''-N-acetyl-6'''-hydroxyneomycin C + O2
2'''-N-acetyl-6'''-oxoneomycin C + H2O2
about 15% of the rate with 6'''-deamino-6'''-hydroxyneomycin C
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2'''-N-acetyl-6'''-hydroxyneomycin C + O2
2'''-N-acetyl-6'''-oxoneomycin C + H2O2
about 15% of the rate with 6'''-deamino-6'''-hydroxyneomycin C
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2'''-N-acetyl-6'''-hydroxyneomycin C + O2
2'''-N-acetyl-6'''-oxoneomycin C + H2O2
less than 10% of the rate with 6'''-deamino-6'''-hydroxyneomycin C
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6'''-deamino-6'''-hydroxyneomycin C + O2
6'''-deamino-6'''-oxoneomycin C + H2O2
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6'''-deamino-6'''-hydroxyneomycin C + O2
6'''-deamino-6'''-oxoneomycin C + H2O2
oxygen-dependent mechanism for the regeneration of the FAD cofactor
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6'''-deamino-6'''-hydroxyneomycin C + O2
6'''-deamino-6'''-oxoneomycin C + H2O2
oxygen-dependent mechanism for the regeneration of the FAD cofactor
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6'''-deamino-6'''-hydroxyneomycin C + O2
6'''-deamino-6'''-oxoneomycin C + H2O2
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6'''-deamino-6'''-hydroxyneomycin C + O2
6'''-deamino-6'''-oxoneomycin C + H2O2
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additional information
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enzyme also catalyzes reaction of EC 1.1.3.43, paromamine 6'-oxidase
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additional information
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enzyme also catalyzes reaction of EC 1.1.3.43, paromamine 6'-oxidase
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additional information
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enzyme does not catalyze the reaction of EC 1.1.3.43, paromamine 6'-oxidase
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3.5
pH 7.5, temperature not specified in the publication
6.4
pH 7.5, temperature not specified in the publication
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UniProt
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UniProt
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UniProt
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physiological function
joint activity of enzyme and 6'-oxoglucosaminyl:L-glutamate aminotransferase Neo-18 is responsible for the conversion of paromamine to neaminein the biosynthetic pathway of neomycin through a mechanism of FAD-dependent dehydrogenation followed by a pyridoxal-5'-phosphate-mediated transamination
physiological function
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joint activity of enzyme and 6'-oxoglucosaminyl:L-glutamate aminotransferase Neo-18 is responsible for the conversion of paromamine to neaminein the biosynthetic pathway of neomycin through a mechanism of FAD-dependent dehydrogenation followed by a pyridoxal-5'-phosphate-mediated transamination
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NEOG_STRFR
541
0
58004
Swiss-Prot
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LIVQ_STRLV
546
0
58907
Swiss-Prot
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60034
x * 60034, LC-ESI-MS
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x * 60034, LC-ESI-MS
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expression in Escherichia coli
expression in Escherichia coli
expression in Escherichia coli
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analysis
development of a coupled enzyme assays including 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazoliumbromide and phenazine methosulfate, resulting in the production of 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazoliumbromide formazan which can be monitored at 570 nm
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Huang, F.; Spiteller, D.; Koorbanally, N.A.; Li, Y.; Llewellyn, N.M.; Spencer, J.B.
Elaboration of neosamine rings in the biosynthesis of neomycin and butirosin
ChemBioChem
8
283-288
2007
Streptomyces fradiae (Q53U15), Streptomyces fradiae NCIMB 8233 (Q53U15)
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Clausnitzer, D.; Piepersberg, W.; Wehmeier, U.F.
The oxidoreductases LivQ and NeoQ are responsible for the different 6-modifications in the aminoglycosides lividomycin and neomycin
J. Appl. Microbiol.
111
642-651
2011
Streptomyces lividus (Q2MF66), Streptomyces fradiae (Q53U15), Streptomyces fradiae NCIMB 8233 (Q53U15)
brenda
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1.1.3.44 6'''-hydroxyneomycin C oxidase
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