BRENDA - Enzyme Database
show all sequences of 1.1.1.371

Bacillus subtilis iolU encodes an additional NADP(+)-dependent scyllo-inositol dehydrogenase

Kang, D.M.; Tanaka, K.; Takenaka, S.; Ishikawa, S.; Yoshida, K.I.; Biosci. Biotechnol. Biochem. 81, 1026-1032 (2017)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene iolW, DNA and amino acid sequence determination and analysis, the gene belongs to the Gfo/Idh/MocA family
Bacillus subtilis
gene yulF, DNA and amino acid sequence determination and analysis, the gene belongs to the Gfo/Idh/MocA family, recombinant expression of His6-tagged YulF
Bacillus subtilis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
scyllo-inositol + NADP+
Bacillus subtilis
-
2,4,6/3,5-pentahydroxycyclohexanone + NADPH + H+
-
-
?
scyllo-inositol + NADP+
Bacillus subtilis 168
-
2,4,6/3,5-pentahydroxycyclohexanone + NADPH + H+
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Bacillus subtilis
O05265
-
-
Bacillus subtilis
O32223
-
-
Bacillus subtilis 168
O05265
-
-
Bacillus subtilis 168
O32223
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
scyllo-inositol + NADP+
-
742123
Bacillus subtilis
2,4,6/3,5-pentahydroxycyclohexanone + NADPH + H+
-
-
-
?
scyllo-inositol + NADP+
recombinant His6-tagged YulF converts scyllo-inosose into SI in an NADPH-dependent manner
742123
Bacillus subtilis
2,4,6/3,5-pentahydroxycyclohexanone + NADPH + H+
-
-
-
?
scyllo-inositol + NADP+
-
742123
Bacillus subtilis 168
2,4,6/3,5-pentahydroxycyclohexanone + NADPH + H+
-
-
-
?
scyllo-inositol + NADP+
recombinant His6-tagged YulF converts scyllo-inosose into SI in an NADPH-dependent manner
742123
Bacillus subtilis 168
2,4,6/3,5-pentahydroxycyclohexanone + NADPH + H+
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
iolU
-
Bacillus subtilis
IolW
-
Bacillus subtilis
NADP+-dependent SI dehydrogenase
-
Bacillus subtilis
yulF
-
Bacillus subtilis
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Bacillus subtilis
Cloned(Commentary) (protein specific)
Commentary
Organism
gene iolW, DNA and amino acid sequence determination and analysis, the gene belongs to the Gfo/Idh/MocA family
Bacillus subtilis
gene yulF, DNA and amino acid sequence determination and analysis, the gene belongs to the Gfo/Idh/MocA family, recombinant expression of His6-tagged YulF
Bacillus subtilis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Bacillus subtilis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
scyllo-inositol + NADP+
Bacillus subtilis
-
2,4,6/3,5-pentahydroxycyclohexanone + NADPH + H+
-
-
?
scyllo-inositol + NADP+
Bacillus subtilis 168
-
2,4,6/3,5-pentahydroxycyclohexanone + NADPH + H+
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
scyllo-inositol + NADP+
-
742123
Bacillus subtilis
2,4,6/3,5-pentahydroxycyclohexanone + NADPH + H+
-
-
-
?
scyllo-inositol + NADP+
recombinant His6-tagged YulF converts scyllo-inosose into SI in an NADPH-dependent manner
742123
Bacillus subtilis
2,4,6/3,5-pentahydroxycyclohexanone + NADPH + H+
-
-
-
?
scyllo-inositol + NADP+
-
742123
Bacillus subtilis 168
2,4,6/3,5-pentahydroxycyclohexanone + NADPH + H+
-
-
-
?
scyllo-inositol + NADP+
recombinant His6-tagged YulF converts scyllo-inosose into SI in an NADPH-dependent manner
742123
Bacillus subtilis 168
2,4,6/3,5-pentahydroxycyclohexanone + NADPH + H+
-
-
-
?
General Information
General Information
Commentary
Organism
evolution
IolW shares a significant similarity with YulF, another NADP+-dependent SI dehydrogenase
Bacillus subtilis
evolution
YulF shares a significant similarity with IolW, another NADP+-dependent SI dehydrogenase
Bacillus subtilis
additional information
when yulF is overexpressed instead of iolW in the Bacillus subtilis cell factory, scyllo-inositol is produced from myo-inositol, suggesting a similar function to iolW
Bacillus subtilis
General Information (protein specific)
General Information
Commentary
Organism
evolution
IolW shares a significant similarity with YulF, another NADP+-dependent SI dehydrogenase
Bacillus subtilis
evolution
YulF shares a significant similarity with IolW, another NADP+-dependent SI dehydrogenase
Bacillus subtilis
additional information
when yulF is overexpressed instead of iolW in the Bacillus subtilis cell factory, scyllo-inositol is produced from myo-inositol, suggesting a similar function to iolW
Bacillus subtilis
Other publictions for EC 1.1.1.371
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742123
Kang
Bacillus subtilis iolU encode ...
Bacillus subtilis, Bacillus subtilis 168
Biosci. Biotechnol. Biochem.
81
1026-1032
2017
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2
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6
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2
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2
2
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4
4
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712965
Morinaga
Identification of two scyllo-i ...
Bacillus subtilis, Bacillus subtilis 168
Microbiology
156
1538-1546
2010
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1
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4
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3
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1
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1
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1
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1
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1
1
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4
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1
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1
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