Cloned (Comment) | Organism |
---|---|
expression in Sf9 cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,2-diheptanoyl-sn-glycero-3-phosphocholine | substitution of the detergent 1,2-diheptanoyl-sn-glycero-3-phosphocholine for Tween-20 results in complete inactivation of the enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0015 | - |
NADPH | - |
Homo sapiens | |
0.0032 | - |
all-trans-retinal | - |
Homo sapiens | |
0.004 | - |
all-trans-retinol | Km-value for all-trans-retinol is similar to that for retinal however, the rate of retinol oxidation by RDH13 is extremely low | Homo sapiens | |
6 | - |
NADH | pH and temperature not specified in the publication. The apparent Km value for NADH is three orders of magnitude higher than that for NADPH | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial membrane | RDH13 is localized on the outer side of the inner mitochondrial membrane | Homo sapiens | 31966 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
all-trans-retinal + NADPH + H+ | Homo sapiens | greater catalytic efficiency in the reductive than in the oxidative direction. Localization of RDH13 at the entrance to the mitochondrial matrix suggests that it may function to protect mitochondria against oxidative stress associated with the highly reactive retinaldehyde produced from dietary beta-carotene | all-trans-retinol + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q8NBN7 | - |
- |
Oxidation Stability | Organism |
---|---|
requires reducing conditions to stay active | Homo sapiens |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | high expression level | Homo sapiens | - |
kidney | high expression level | Homo sapiens | - |
lung | high expression level | Homo sapiens | - |
ovary | moderate expression level | Homo sapiens | - |
prostate | moderate expression level | Homo sapiens | - |
testis | moderate expression level | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
all-trans-retinal + NADPH + H+ | greater catalytic efficiency in the reductive than in the oxidative direction. Localization of RDH13 at the entrance to the mitochondrial matrix suggests that it may function to protect mitochondria against oxidative stress associated with the highly reactive retinaldehyde produced from dietary beta-carotene | Homo sapiens | all-trans-retinol + NADP+ | - |
? | |
all-trans-retinal + NADPH + H+ | prefers NADPH to NADH as a cofactor. Activity in presence of 1 mM NADPH is about 20fold greater than that in the presence of 1 mM NADH | Homo sapiens | all-trans-retinol + NADP+ | - |
? | |
additional information | no significant conversion of 17beta-, 3alpha- and 11beta-hydroxysteroids | Homo sapiens | ? | - |
? | |
retinal + NADH + H+ | - |
Homo sapiens | retinol + NAD+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
RDH13 | - |
Homo sapiens |
retinol dehydrogenase 13 | - |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
partially inactivated after 20 min | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | prefers NADPH to NADH as a cofactor. Activity in presence of 1 mM NADPH is about 20fold greater than that in the presence of 1 mM NADH | Homo sapiens | |
NADPH | prefers NADPH to NADH as a cofactor. Activity in presence of 1 mM NADPH is about 20fold greater than that in the presence of 1 mM NADH | Homo sapiens |