Crystallization (Comment) | Organism |
---|---|
purified recombinant C-terminally His8-tagged and selenomethionine-labeled enzyme, subunit A is bound with NAD+ and a phosphate ion, while subunit B, with a more open active site cleft, is bound with NAD+ and L(+)-tartrate, cryoprotection by 30% glycerol, X-ray diffraction structure determination and analysis at 2.3 A resolution, asymmetric subunit conformation and strucure comparison, overview | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-phospho-D-erythronate + NAD+ | Pseudomonas aeruginosa | the enzyme is involved in biosynthesis of pyridoxal-5'-phosphate | (3R)-3-hydroxy-2-oxo-4-phosphooxybutanoate + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9I3W9 | gene pdxB | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4-phospho-D-erythronate + NAD+ = (3R)-3-hydroxy-2-oxo-4-phosphooxybutanoate + NADH + H+ | substrate recognition and catalytic mechanism, PdxB contains the conserved His254/Glu237/Arg208 triad | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-phospho-D-erythronate + NAD+ | - |
Pseudomonas aeruginosa | (3R)-3-hydroxy-2-oxo-4-phosphooxybutanoate + NADH | - |
? | |
4-phospho-D-erythronate + NAD+ | the enzyme is involved in biosynthesis of pyridoxal-5'-phosphate | Pseudomonas aeruginosa | (3R)-3-hydroxy-2-oxo-4-phosphooxybutanoate + NADH | - |
? | |
additional information | active site and ligand binding structure, overview | Pseudomonas aeruginosa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | homodimer, each subunit consists of three structural domains: the lid domain, the nucleotide-binding domain, and the C-terminal dimerization domain with a unique fold responsible for the dimerization, crystal structure analysis, overview | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
D-erythronate-4-phosphate dehydrogenase | - |
Pseudomonas aeruginosa |
More | PdxB is a member of the D-isomer specific 2-hydroxyacid dehydrogenase superfamily | Pseudomonas aeruginosa |
PDXB | - |
Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | NAD+ is bound to the C-terminal side of the beta-sheet of the nucleotide-binding domain in both subunits of PdxB, binding structure, overview | Pseudomonas aeruginosa |