General Stability | Organism |
---|---|
NAD+ and NADH protect from denaturation by high temperatures, acid pH and storage at 0°C | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP-ribose | - |
Homo sapiens | |
Alpha-NAD+ | - |
Homo sapiens | |
Hg2+ | - |
Homo sapiens | |
NAD+ | competitive, nonlinear inhibitor, when the concentration of NADH varies at constant S-formylglutathione concentration, noncompetitive inhibitor when the concentration of S-formylglutathione is varied at constant NADH concentration | Homo sapiens | |
p-hydroxymercuribenzoate | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0042 | - |
NADH | pH 5.7 | Homo sapiens | |
0.008 | - |
glutathione | pH 8 | Homo sapiens | |
0.009 | - |
formaldehyde | pH 8 | Homo sapiens | |
0.045 | - |
NADPH | pH 5.7 | Homo sapiens | |
0.3 | - |
S-formylglutathione | pH 5.7, with NADH as cofactor | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.17 | - |
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
0°C, half-life in absence of a stabilizer is a few h, in presence of 1 mM NAD+ or 40% v/v glycerol the half life is about 1 month, 0.5 mM NADH is even more effective | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-ethoxy-2-hydroxybutyraldehyde + glutathione + NAD+ | weak activity | Homo sapiens | S-(3-ethoxy-2-hydroxybutyryl)-glutathione + NADH + H+ | - |
? | |
formaldehyde + glutathione + NAD+ | at pH 8 the rate of the reverse reaction with S-formylglutathione and NADH is about the same as that of the forward reaction with formaldehyde, glutathione and NAD+. At pH 5.7 the rate of the reverse reaction with S-formylglutathione and NADH is 3.9times and with S-formylglutathione and NADPH 2.0times, that of the forward reaction rate with NAD+ at pH 8.0 | Homo sapiens | S-formylglutathione + NADH + H+ | - |
? | |
formaldehyde + glutathione + NAD+ | at pH 8 the rate of the reverse reaction with S-formylglutathione and NADH is about the same as that of the forward reaction with formaldehyde, glutathione and NAD+. At pH 5.7 the rate of the reverse reaction with S-formylglutathione and NADH is 3.9times and with S-formylglutathione and NADPH 2.0times, that of the forward reaction rate with NAD+ at pH 8.0 | Homo sapiens | S-formylglutathione + NADH + H+ | - |
r | |
formaldehyde + glutathione + NADP+ | - |
Homo sapiens | S-formylglutathione + NADPH + H+ | - |
r | |
glyoxal + glutathione + NAD+ | weak activity | Homo sapiens | S-oxoacetylglutathione + NADH | - |
? | |
hydroxypyruvaldehyde + glutathione + NAD+ | weak activity | Homo sapiens | S-hydroxypyruvylglutathione + NADH | - |
? | |
methylglyoxal + glutathione + NAD+ | 85% of the activity with formaldehyde | Homo sapiens | S-pyruvylglutathione + NADH | - |
? | |
S-formylglutathione + NADH | - |
Homo sapiens | formaldehyde + glutathione + NAD+ | - |
r | |
S-formylglutathione + NADPH | - |
Homo sapiens | formaldehyde + glutathione + NADP+ | - |
r | |
S-pyruvylglutathione + NADH | - |
Homo sapiens | methylglyoxal + glutathione + NAD+ | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.7 | - |
reaction with S-formylglutathione and NADH | Homo sapiens |
8 | - |
oxidation of formaldehyde | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Homo sapiens | |
NADH | - |
Homo sapiens | |
NADP+ | at pH 8 the Km-value is about 1000times greater than that for NAD+. At pH 6 NADP+ is used better but this reaction appears to be without physiological significance | Homo sapiens | |
NADPH | - |
Homo sapiens |