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Literature summary for 1.1.1.262 extracted from

  • Banks, J.; Cane, D.E.
    Biosynthesis of vitamin B6: direct identification of the product of the PdxA-catalyzed oxidation of 4-hydroxy-L-threonine-4-phosphate using electrospray ionization mass spectrometry (2004), Bioorg. Med. Chem. Lett., 14, 1633-16367.
    View publication on PubMed

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ tightly bound, divalent metal ion-dependent enzyme, Zn2+ can be replaced by e.g. Mg2+ Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-(phosphonooxy)threonine + NAD(P)+ Escherichia coli involved in the biosynthesis of vitamin B6 3-amino-1-hydroxyacetone 1-phosphate + CO2 + NAD(P)H
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Reaction

Reaction Comment Organism Reaction ID
4-phosphooxy-L-threonine + NAD+ = 3-amino-2-oxopropyl phosphate + CO2 + NADH + H+ 3-amino-1-hydroxyacetone 1-phosphate,i.e. AHAB, is the primary product of the reaction, no formation of 2-amino-3-oxo-4-phosphonooxybutyrate as intermediate is detected, identification by electrospray ionization mass spectrometry Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-(phosphonooxy)threonine + NAD(P)+ involved in the biosynthesis of vitamin B6 Escherichia coli 3-amino-1-hydroxyacetone 1-phosphate + CO2 + NAD(P)H
-
?
4-(phosphonooxy)threonine + NAD(P)+ i.e. 4-hydroxy-L-threonine-4-phosphate, HTP Escherichia coli 3-amino-1-hydroxyacetone 1-phosphate + CO2 + NAD(P)H i.e. AHAB, primary product of the reaction, no formation of 2-amino-3-oxo-4-phosphonooxybutyrate as intermediate is detected, identification by electrospray ionization mass spectrometry ?

Synonyms

Synonyms Comment Organism
PdxA
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NAD(P)+ dependent on Escherichia coli