3-amino-1-hydroxyacetone 1-phosphate,i.e. AHAB, is the primary product of the reaction, no formation of 2-amino-3-oxo-4-phosphonooxybutyrate as intermediate is detected, identification by electrospray ionization mass spectrometry
Substrates: involved in pyridoxal 5'-phosphate formation Products: further condensation with 1-deoxy-D-xylulose-5-phosphate to form pyridoxal 5'-phosphate
Substrates: involved in pyridoxal 5'-phosphate formation Products: further condensation with 1-deoxy-D-xylulose-5-phosphate to form pyridoxal 5'-phosphate
Substrates: involved in pyridoxal 5'-phosphate formation Products: undergoes decarboxylation either catalysed by the enzyme or after release, further formation of pyridoxal 5'-phosphate catalysed by PdxJ protein
Substrates: i.e. 4-hydroxy-L-threonine phosphate or HTP, enzyme strictly requires the phosphate ester form of the substrate, interaction between enzyme and substrate via phosphate group, step 1 of the overall reaction Products: reaction intermediate
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4-(phosphonooxy)threonine + NAD(P)+
3-amino-1-hydroxyacetone 1-phosphate + CO2 + NAD(P)H
Substrates: i.e. 4-hydroxy-L-threonine-4-phosphate, HTP Products: i.e. AHAB, primary product of the reaction, no formation of 2-amino-3-oxo-4-phosphonooxybutyrate as intermediate is detected, identification by electrospray ionization mass spectrometry
Substrates: involved in pyridoxal 5'-phosphate formation Products: further condensation with 1-deoxy-D-xylulose-5-phosphate to form pyridoxal 5'-phosphate
Substrates: involved in pyridoxal 5'-phosphate formation Products: further condensation with 1-deoxy-D-xylulose-5-phosphate to form pyridoxal 5'-phosphate
Substrates: involved in pyridoxal 5'-phosphate formation Products: undergoes decarboxylation either catalysed by the enzyme or after release, further formation of pyridoxal 5'-phosphate catalysed by PdxJ protein
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
8.1 mg/ml purified recombinant enzyme in complex with substrate 4-(phosphonooxy)threonine and Zn2+ in 20 mM Tris-HCl, pH 8.0, 0.2 M NaCl, 10 mM DTT, 5% w/v glycerol, hanging drop vapour diffusion method, 0.002 ml protein solution with 0.004 ml reservoir solution, usage of 2 reservoir solution variants resulting in 2 differen crystal forms, solution 1 contains 7.5% w/v PEG 8000, 0.1 M NaOAc, pH 5.5, 10 mM MgCl2, 10 mM NaKHPO4, pH 5.9, solution 2 contains 20% w/v PEG 8000, 100 mM HEPES, pH 7.5, 75 mM citrate, and 100 mM MgCl2, suspension over reservoir solution, X-ray diffraction structure determination and analysis at 2,0 and 2.45 A resolution, respectively
Vitamin B6 biosynthesis: formation of pyridoxine 5'-phosphate from 4-(phosphohydroxy)-L-threonine and 1-deoxy-D-xylulose-5-phosphate by PdxA and PdxJ protein
Biosynthesis of vitamin B6: direct identification of the product of the PdxA-catalyzed oxidation of 4-hydroxy-L-threonine-4-phosphate using electrospray ionization mass spectrometry
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