Application | Comment | Organism |
---|---|---|
analysis | usage of Escherichia coli shikimate dehydrogenase as sensor reaction for determination of the cytosolic NADPH/NADP ratio in Saccharomyces cerevisiae, quantitative measurements of physiological variables in the cytosolic compartment by GC-MS/MS, cytosolic NADPH/NADP ratio in batch experiments, overview. The steady state sensor reaction based cytosolic free NADPH/NADP ratio is 15.6 | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
gene aroE from Escherichia coli sstrain DH5alpha, recombinant overexpression in Saccharomyces cerevisiae strain CEN.PK 113-5D yielding strain CEN.PK-aroE | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | steady state cytosolic free and whole cell NADPH/NADP ratio in different Saccharomycs cerevisiae strains, with or without recombinant expression of shikimate dehydrogenase from Escherichia coli, thermodynamics and kinetics, overview | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | occurs only in the cytosol | Escherichia coli | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
shikimate + NADP+ | Escherichia coli | - |
3-dehydroshikimate + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P15770 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.68 | - |
recombinant CEN.PK-aroE strain expressing the enzyme from Eschericha coli, pH 7.0, 25°C | Escherichia coli |
19.9 | - |
pH 9.0, 25°C, recombinant enzyme | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | usage of recombinant shikimate dehydrogenase as sensor reaction for determination of the cytosolic NADPH/NADP ratio in Saccharomyces cerevisiae, quantitative measurements of physiological variables in the cytosolic compartment by GC-MS/MS, cytosolic NADPH/NADP ratio in batch experiments, method, overview | Escherichia coli | ? | - |
? | |
shikimate + NADP+ | - |
Escherichia coli | 3-dehydroshikimate + NADPH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
AroE | - |
Escherichia coli |
SDH | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
30 | - |
in vivo assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
in vivo assay at | Escherichia coli |
9 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | specific for | Escherichia coli | |
NADPH | specific for | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
additional information | modelling of steady state and dynamic fluxes into pentose phosphate pathway and the flux split ratio into glycolysis and pentose phosphate pathway in Saccharomyces recombinantly expressing Escherichia coli shikimate dehydrogenase, overview | Escherichia coli |