Cloned (Comment) | Organism |
---|---|
TRII cDNA, in Escherichia coli expression vector pET21d | Datura stramonium |
TRII cDNA, in Escherichia coli expression vector pET21d | Hyoscyamus niger |
Crystallization (Comment) | Organism |
---|---|
- |
Datura stramonium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
3 * 29000, SDS-PAGE | Hyoscyamus niger |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tropinone + NADPH + H+ | Datura stramonium | - |
pseudotropine + NADP+ | - |
? | |
tropinone + NADPH + H+ | Atropa belladonna | - |
pseudotropine + NADP+ | - |
? | |
tropinone + NADPH + H+ | Hyoscyamus niger | - |
pseudotropine + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Atropa belladonna | - |
- |
- |
Datura stramonium | - |
- |
- |
Hyoscyamus niger | - |
- |
- |
no activity in Nicotiana tabacum | - |
tobacco | - |
Purification (Comment) | Organism |
---|---|
- |
Datura stramonium |
- |
Hyoscyamus niger |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
endodermis | - |
Hyoscyamus niger | - |
root | - |
Datura stramonium | - |
root | - |
Atropa belladonna | - |
root | - |
Hyoscyamus niger | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tropinone + NADPH + H+ | - |
Datura stramonium | pseudotropine + NADP+ | - |
? | |
tropinone + NADPH + H+ | - |
Atropa belladonna | pseudotropine + NADP+ | - |
? | |
tropinone + NADPH + H+ | - |
Hyoscyamus niger | pseudotropine + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | - |
Datura stramonium |
trimer | - |
Atropa belladonna |
trimer | 3 * 29000, SDS-PAGE | Hyoscyamus niger |