Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine diphosphoribose | competitive inhibition with respect to NADH, noncompetitive inhibition with respect to D-fructose 6-phosphate | Aspergillus niger | |
D-fructose 6-phosphate | competitive inhibition with respect to D-mannitol 1-phosphate | Aspergillus niger | |
D-glucose 6-phosphate | competitive inhibition with respect to D-fructose 6-phosphate, noncompetitive inhibition with respect to NADH | Aspergillus niger | |
D-mannitol 1-phosphate | competitive inhibition with respect to D-fructose 6-phosphate, noncompetitive inhibition with respect to NADH | Aspergillus niger | |
NAD+ | competitive inhibition with respect to NADH, noncompetitive inhibition with respect to D-fructose 6-phosphate | Aspergillus niger | |
NADH | competitive inhibition with respect to NAD+ | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
NADH | cosubstrate: D-fructose 6-phosphate | Aspergillus niger | |
0.038 | - |
D-mannitol 1-phosphate | cosubstrate: NAD+ | Aspergillus niger | |
0.083 | - |
NAD+ | cosubstrate: D-mannnitol 1-phosphate | Aspergillus niger | |
0.54 | - |
D-fructose 6-phosphate | cosubstrate: NADH | Aspergillus niger |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Aspergillus niger | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22000 | - |
2 * 22000, SDS-PAGE | Aspergillus niger |
40000 | - |
gel filtration | Aspergillus niger |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol 1-phosphate + NAD+ | Aspergillus niger | highly specific for both substrates | D-fructose 6-phosphate + NADH + H+ | - |
r | |
D-mannitol 1-phosphate + NAD+ | Aspergillus niger | random bi-bi kinetic with two dead-end complexes | D-fructose 6-phosphate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography techniques | Aspergillus niger |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Aspergillus niger | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
150 | - |
- |
Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol 1-phosphate + acetylpyridine adenine dinucleotide | 50% of the activity compared to NAD+ | Aspergillus niger | D-fructose 6-phosphate + ? | - |
? | |
D-mannitol 1-phosphate + NAD+ | highly specific for both substrates | Aspergillus niger | D-fructose 6-phosphate + NADH + H+ | - |
r | |
D-mannitol 1-phosphate + NAD+ | random bi-bi kinetic with two dead-end complexes | Aspergillus niger | D-fructose 6-phosphate + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 22000, SDS-PAGE | Aspergillus niger |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10 | more than 95% of its original activity | Aspergillus niger |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | strictly specific | Aspergillus niger | |
NADH | - |
Aspergillus niger |