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D-fructose 6-phosphate + NADH + H+
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADPH + H+
D-mannitol 1-phosphate + NADP+
D-fructose-6-phosphate + NADH
D-mannitol-1-phosphate + NAD+
-
-
-
r
D-mannitol 1-phosphate + 3-acetylpyridine-NAD+
D-fructose 6-phosphate + ?
-
7% of the activity compared to NAD+
-
?
D-mannitol 1-phosphate + acetylpyridine adenine dinucleotide
D-fructose 6-phosphate + ?
-
50% of the activity compared to NAD+
-
?
D-mannitol 1-phosphate + dichlorophenolindophenol
D-fructose 6-phosphate + reduced dichlorophenolindophenol
-
higher activity than for NAD+
-
?
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
D-mannitol 1-phosphate + NADP+
D-fructose 6-phosphate + NADPH + H+
D-mannitol 1-phosphate + nicotinamide 6-(2-hydoxyethylamino) purine dinucleotide
D-fructose 6-phosphate + ?
-
75% of the activity compared to NAD+
-
?
D-mannitol 1-phosphate + nicotinamide hypoxanthine dinucleotide
D-fructose 6-phosphate + ?
-
84% of the activity compared to NAD+
-
?
D-mannitol-1-phosphate + NAD+
D-fructose-6-phosphate + NADH
-
-
-
r
D-sorbitol 6-phosphate + NAD+
D-glucose 6-phosphate + NADH + H+
-
38.8% of the activity compared to D-mannitol 1-phosphate
-
r
ethanol + NAD+
acetaldehyde + NADH
-
56% of the activity compared to D-mannitol 1-phosphate
-
r
hexitol phosphate + NAD+
? + NADH
additional information
?
-
D-fructose 6-phosphate + NADH + H+

D-mannitol 1-phosphate + NAD+
-
-
-
r
D-fructose 6-phosphate + NADH + H+
D-mannitol 1-phosphate + NAD+
-
-
-
r
D-fructose 6-phosphate + NADH + H+
D-mannitol 1-phosphate + NAD+
-
-
r
D-fructose 6-phosphate + NADPH + H+

D-mannitol 1-phosphate + NADP+
-
-
-
r
D-fructose 6-phosphate + NADPH + H+
D-mannitol 1-phosphate + NADP+
-
-
r
D-mannitol 1-phosphate + NAD+

D-fructose 6-phosphate + NADH + H+
-
-
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
the enzyme is involved in mannitol biosynthesis, which is required for plant pathogenicity by Alternaria alternata, overview
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
-
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
the enzyme is involved in mannitol biosynthesis, which is required for plant pathogenicity by Alternaria alternata, overview
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
-
-
?
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
-
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
M1PDH is stereospecific for transferring the hydrogen to NAD+, in situ proton NMR studies of enzymatic oxidation of D-5-[2H]-mannitol 1-phosphate, overview
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
AfM1PDH primarily functions as a D-fructose-6-phosphate reductase and is specific for its natural pair of substrates
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
-
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
-
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
-
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
highly specific for both substrates
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
random bi-bi kinetic with two dead-end complexes
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
-
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
-
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
-
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
the enzyme shows higher activity in the reduction reaction
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
the enzyme shows higher activity in the reduction reaction
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
part of the phosphoenolpyruvate phosphotransferase system
-
?
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
-
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
highly specific for both substrates
-
?
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
highly specific for both substrates
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
favours reverse reaction
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
highly specific for both substrates
-
?
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
-
-
?
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
-
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
-
-
?
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
-
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
the enzyme competes with two sorbitol-6-phosphate dehydrogenase for D-fructose 6-phosphate, metabolic flux in engineered organisms, overview
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
-
-
?
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
-
-
?
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
highly specific for both substrates
-
?
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
part of the phosphoenolpyruvate phosphotransferase system
-
?
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
-
-
r
D-mannitol 1-phosphate + NADP+

D-fructose 6-phosphate + NADPH + H+
-
-
r
D-mannitol 1-phosphate + NADP+
D-fructose 6-phosphate + NADPH + H+
-
-
r
hexitol phosphate + NAD+

? + NADH
-
-
-
?
hexitol phosphate + NAD+
? + NADH
-
-
-
?
additional information

?
-
MtlD is a bifunctional enzyme of mannitol biosynthesis that combines mannitol-1-phosphate dehydrogenase and phosphatase activities in a single polypeptide chain
-
?
additional information
?
-
MtlD is a bifunctional enzyme of mannitol biosynthesis that combines mannitol-1-phosphate dehydrogenase and phosphatase activities in a single polypeptide chain
-
?
additional information
?
-
-
important role for Lys213 in the catalytic mechanism of M1PDH
-
?
additional information
?
-
-
M1PDH does not catalyze the oxidation of D-mannitol, D-sorbitol, D-ribitol, xylitol, D-xylose, L-xylose, D-glucose, D-mannose, L-arabinose, D-arabinose, D-galactose, L-fucose, and D-lyxose. The enzyme is also inactive above a level of 1% activity with D-fructose 6-phosphate for reduction of D-fructose, L-sorbose, D-xylulose, D-fructose 1,6-bisphosphate, D-glucose 6-phosphate, and D-glucose 1-phosphate
-
?
additional information
?
-
-
required for assimilation of mannitol and glucitol
-
?
additional information
?
-
-
high specificity for substrates fructose-6-phosphate/NADH and mannitol-1-phosphate/NAD+, no substrate: fructose-1-phosphate, fructose-1,6-bisphosphate, glucose-1-phosphate, NADPH, NADP+
-
?
additional information
?
-
mannitol is present in large amounts in brown algae, where its synthesis involved two steps: a mannitol-1-phosphate dehydrogenase (M1PDH) catalyzes a reversible reaction between fructose-6-phosphate (F6P) and mannitol-1-phosphate (M1P) (EC 1.1.1.17), and a mannitol-1-phosphatase hydrolyzes M1P to mannitol (EC 3.1.3.22)
-
?
additional information
?
-
mannitol is present in large amounts in brown algae, where its synthesis involved two steps: a mannitol-1-phosphate dehydrogenase (M1PDH) catalyzes a reversible reaction between fructose-6-phosphate (F6P) and mannitol-1-phosphate (M1P) (EC 1.1.1.17), and a mannitol-1-phosphatase hydrolyzes M1P to mannitol (EC 3.1.3.22)
-
?
additional information
?
-
mannitol is present in large amounts in brown algae, where its synthesis involved two steps: a mannitol-1-phosphate dehydrogenase (M1PDH) catalyzes a reversible reaction between fructose-6-phosphate (F6P) and mannitol-1-phosphate (M1P) (EC 1.1.1.17), and a mannitol-1-phosphatase hydrolyzes M1P to mannitol (EC 3.1.3.22)
-
?
additional information
?
-
EsM1PDH1cat isozyme is specific for D-fructose 6-phosphate and D-mannitol 1-phosphate
-
?
additional information
?
-
EsM1PDH1cat isozyme is specific for D-fructose 6-phosphate and D-mannitol 1-phosphate
-
?
additional information
?
-
EsM1PDH1cat isozyme is specific for D-fructose 6-phosphate and D-mannitol 1-phosphate
-
?
additional information
?
-
no activity with D-glucose 6-phosphate, sorbitol 6-phosphate, D-fructose 1-phosphate
-
?
additional information
?
-
-
no activity with D-glucose 6-phosphate, sorbitol 6-phosphate, D-fructose 1-phosphate
-
?
additional information
?
-
-
Petunia hybrida (Hook) Vilm. cv. Mitchell is transformed with an Escherichia coli gene encoding mannitol 1-phosphate dehydrogenase. The high-mannitol containing lines are more tolerant of chilling stress than the low mannitol containing transgenic lines and wild-type. In the higher mannitol lines only 0.04% to 0.06% of the total osmotic potential generated from all solutes can be attributed to mannitol, thus its action is more like that of an osmoprotectant rather than an osmoregulator
-
?
additional information
?
-
mannitol 1-phosphate metabolism is required for sporulation in planta of the wheat pathogen Stagonospora nodorum
-
?
additional information
?
-
-
mannitol 1-phosphate metabolism is required for sporulation in planta of the wheat pathogen Stagonospora nodorum
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
D-fructose 6-phosphate + NADH + H+
D-mannitol 1-phosphate + NAD+
-
-
-
-
r
D-fructose 6-phosphate + NADPH + H+
D-mannitol 1-phosphate + NADP+
-
-
-
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
D-mannitol 1-phosphate + NADP+
D-fructose 6-phosphate + NADPH + H+
D-sorbitol 6-phosphate + NAD+
D-glucose 6-phosphate + NADH + H+
-
38.8% of the activity compared to D-mannitol 1-phosphate
-
r
ethanol + NAD+
acetaldehyde + NADH
-
56% of the activity compared to D-mannitol 1-phosphate
-
r
hexitol phosphate + NAD+
? + NADH
additional information
?
-
D-mannitol 1-phosphate + NAD+

D-fructose 6-phosphate + NADH + H+
-
the enzyme is involved in mannitol biosynthesis, which is required for plant pathogenicity by Alternaria alternata, overview
-
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
the enzyme is involved in mannitol biosynthesis, which is required for plant pathogenicity by Alternaria alternata, overview
-
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
-
-
?
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
-
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
AfM1PDH primarily functions as a D-fructose-6-phosphate reductase and is specific for its natural pair of substrates
-
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
-
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
-
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
-
-
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
highly specific for both substrates
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
random bi-bi kinetic with two dead-end complexes
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
-
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
-
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
-
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
-
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
-
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
the enzyme shows higher activity in the reduction reaction
-
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
the enzyme shows higher activity in the reduction reaction
-
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
part of the phosphoenolpyruvate phosphotransferase system
-
?
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
-
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
highly specific for both substrates
-
?
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
highly specific for both substrates
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
favours reverse reaction
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
highly specific for both substrates
-
?
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
-
-
?
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
-
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
-
-
?
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
the enzyme competes with two sorbitol-6-phosphate dehydrogenase for D-fructose 6-phosphate, metabolic flux in engineered organisms, overview
-
-
r
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
-
-
?
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
-
-
?
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
highly specific for both substrates
-
?
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
part of the phosphoenolpyruvate phosphotransferase system
-
?
D-mannitol 1-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
-
-
-
-
r
D-mannitol 1-phosphate + NADP+

D-fructose 6-phosphate + NADPH + H+
-
-
-
r
D-mannitol 1-phosphate + NADP+
D-fructose 6-phosphate + NADPH + H+
-
-
-
r
hexitol phosphate + NAD+

? + NADH
-
-
-
?
hexitol phosphate + NAD+
? + NADH
-
-
-
?
additional information

?
-
MtlD is a bifunctional enzyme of mannitol biosynthesis that combines mannitol-1-phosphate dehydrogenase and phosphatase activities in a single polypeptide chain
-
-
?
additional information
?
-
MtlD is a bifunctional enzyme of mannitol biosynthesis that combines mannitol-1-phosphate dehydrogenase and phosphatase activities in a single polypeptide chain
-
-
?
additional information
?
-
-
required for assimilation of mannitol and glucitol
-
-
?
additional information
?
-
mannitol is present in large amounts in brown algae, where its synthesis involved two steps: a mannitol-1-phosphate dehydrogenase (M1PDH) catalyzes a reversible reaction between fructose-6-phosphate (F6P) and mannitol-1-phosphate (M1P) (EC 1.1.1.17), and a mannitol-1-phosphatase hydrolyzes M1P to mannitol (EC 3.1.3.22)
-
-
?
additional information
?
-
mannitol is present in large amounts in brown algae, where its synthesis involved two steps: a mannitol-1-phosphate dehydrogenase (M1PDH) catalyzes a reversible reaction between fructose-6-phosphate (F6P) and mannitol-1-phosphate (M1P) (EC 1.1.1.17), and a mannitol-1-phosphatase hydrolyzes M1P to mannitol (EC 3.1.3.22)
-
-
?
additional information
?
-
mannitol is present in large amounts in brown algae, where its synthesis involved two steps: a mannitol-1-phosphate dehydrogenase (M1PDH) catalyzes a reversible reaction between fructose-6-phosphate (F6P) and mannitol-1-phosphate (M1P) (EC 1.1.1.17), and a mannitol-1-phosphatase hydrolyzes M1P to mannitol (EC 3.1.3.22)
-
-
?
additional information
?
-
-
Petunia hybrida (Hook) Vilm. cv. Mitchell is transformed with an Escherichia coli gene encoding mannitol 1-phosphate dehydrogenase. The high-mannitol containing lines are more tolerant of chilling stress than the low mannitol containing transgenic lines and wild-type. In the higher mannitol lines only 0.04% to 0.06% of the total osmotic potential generated from all solutes can be attributed to mannitol, thus its action is more like that of an osmoprotectant rather than an osmoregulator
-
-
?
additional information
?
-
mannitol 1-phosphate metabolism is required for sporulation in planta of the wheat pathogen Stagonospora nodorum
-
-
?
additional information
?
-
-
mannitol 1-phosphate metabolism is required for sporulation in planta of the wheat pathogen Stagonospora nodorum
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1,10-phenanthroline
-
weak inhibition
2,2'-bipyridyl
-
weak inhibition
4,7-phenanthroline
-
weak inhibition
4-hydroxymercuribenzoate
strong inhibition; strong inhibition; strong inhibition
5,5'-dithiobis-(2-nitrobenzoate)
-
complete inactivation at 0.24 mM
adenosine
-
48% inhibition of fructose 6-phosphate reduction
adenosine diphosphoribose
-
competitive inhibition with respect to NADH, noncompetitive inhibition with respect to D-fructose 6-phosphate
Ca2+
35.9% inhibition at 10 mM of the reverse reaction
cytidine
-
17% inhibition of fructose 6-phosphate reduction
D-fructose 1,6-diphosphate
-
12% inhibition of mannitol 1-phosphate oxidation
D-glucose 6-phosphate
-
competitive inhibition with respect to D-fructose 6-phosphate, noncompetitive inhibition with respect to NADH
D-mannitol 1-phosphate
-
competitive inhibition with respect to D-fructose 6-phosphate, noncompetitive inhibition with respect to NADH
diethyldicarbonate
-
60% inhibition at 1 mM
diethyldithiocarbamate
-
95% inactivation, prevented by presence of 2-mercaptoethanol
DTT
46.0% inhibition at 10 mM of the reverse reaction
fructose-6-phosphate
-
substrate inhibition above 0.5 mM
GTP
-
11% inhibition of mannitol 1-phosphate oxidation
guanosine
-
10% inhibition of fructose 6-phosphate reduction
Li+
24.4% inhibition at 50 mM of the reverse reaction
Mg2+
31.2% inhibition at 10 mM of the reverse reaction
Mn2+
31.3% inhibition at 50 mM of the reverse reaction
N-ethylmaleimide
-
1 mM, 80% inhibition of reduction and oxidation reaction
NAD+
-
competitive inhibition with respect to NADH, noncompetitive inhibition with respect to D-fructose 6-phosphate
p-hydroxymercuribenzoate
-
0.5 mM, complete inhibition of reduction and oxidation reaction, 2-mercaptoethanol protects
phosphoenolpyruvate
-
14% inhibition of mannitol 1-phosphate oxidation
SDS
80.1% inhibition at 1 mM of the reverse reaction
thymidine
-
8% inhibition of fructose 6-phosphate reduction
uridine
-
16% inhibition of fructose 6-phosphate reduction
ADP

-
competitive inhibition
ADP
-
20% inhibition of mannitol 1-phosphate oxidation
ATP

-
competitive inhibition
ATP
-
53% inhibition of mannitol 1-phosphate oxidation
Cd2+

-
competitive inhibition
Cd2+
-
cooperative binding
Co2+

-
competitive inhibition
Co2+
-
cooperative binding
Cu2+

-
irreversible inactivation
Cu2+
-
irreversible inactivation
Cu2+
32.8% inhibition at 2 mM of the reverse reaction
D-fructose 6-phosphate

-
competitive inhibition with respect to D-mannitol 1-phosphate
D-fructose 6-phosphate
-
56% inhibition of mannitol 1-phosphate oxidation
Hg2+

-
irreversible inactivation
Hg2+
-
irreversible inactivation
NADH

-
-
NADH
-
competitive inhibition with respect to NAD+
Ni2+

-
competitive inhibition
Ni2+
-
cooperative binding
Zn2+

-
competitive with respect to D-fructose 6-phosphate, noncompetitive inhibition with respect to NADH
Zn2+
-
cooperative binding of Zn2+, competitive type if KCl concentration is greater 100 mM, sigmoidal type at lower salt concentrations
Zn2+
44.7% inhibition at 2 mM of the reverse reaction
additional information

-
poor effects by ATP, ADP and AMP
-
additional information
no or poor inhibition of the reverse reaction by EDTA and 2-mercaptoethanol
-
additional information
-
no or poor inhibition of the reverse reaction by EDTA and 2-mercaptoethanol
-
additional information
no inhibition by EDTA; no inhibition by EDTA; no inhibition by EDTA
-
additional information
no inhibition by EDTA; no inhibition by EDTA; no inhibition by EDTA
-
additional information
no inhibition by EDTA; no inhibition by EDTA; no inhibition by EDTA
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.19 - 13.6
D-fructose 6-phosphate
188.8 - 1458
D-fructose-6-phosphate
0.038 - 2
D-mannitol 1-phosphate
48.9 - 138
D-mannitol-1-phosphate
0.94
ethanol
-
cosubstrate: NAD+
0.8
hexitol phosphate
-
cosubstrate: NAD+
additional information
additional information
-
0.19
D-fructose 6-phosphate

recombinant enzyme, pH 7.0, 30°C
0.2
D-fructose 6-phosphate
cofactor NADH, 41°C, pH 7.5
0.25
D-fructose 6-phosphate
-
cosubstrate: NADH
0.3
D-fructose 6-phosphate
-
cosubstrate: NADH
0.3
D-fructose 6-phosphate
cofactor NADPH, 41°C, pH 7.5
0.54
D-fructose 6-phosphate
-
cosubstrate: NADH
1.66
D-fructose 6-phosphate
-
cosubstrate: NADH
2.1
D-fructose 6-phosphate
-
cosubstrate: NADH
2.1
D-fructose 6-phosphate
pH 7.1, 25°C, recombinant enzyme
3.2
D-fructose 6-phosphate
-
pH 10.0, 25°C, recombinant enzyme
13.6
D-fructose 6-phosphate
-
pH 7.0, 30°C
188.8
D-fructose-6-phosphate

-
pH 7.0, 25°C
1458
D-fructose-6-phosphate
-
pH 7.0, 25°C, presence of 200 mM NaCl
0.038
D-mannitol 1-phosphate

-
cosubstrate: NAD+
0.05
D-mannitol 1-phosphate
-
cosubstrate: NAD+
0.055
D-mannitol 1-phosphate
-
cosubstrate: NAD+
0.13
D-mannitol 1-phosphate
-
pH 7.1, 25°C, recombinant enzyme
0.15
D-mannitol 1-phosphate
-
cosubstrate: NAD+
0.23
D-mannitol 1-phosphate
pH 7.1, 25°C, recombinant enzyme
0.38
D-mannitol 1-phosphate
recombinant enzyme, pH 9.0, 30°C
2
D-mannitol 1-phosphate
-
cosubstrate: NAD+
48.9
D-mannitol-1-phosphate

-
pH 7.0, 25°C
138
D-mannitol-1-phosphate
-
pH 7.0, 25°C, presence of 200 mM NaCl
0.02
NAD+

recombinant enzyme, pH 9.0, 30°C
0.06
NAD+
-
cosubstrate: D-mannitol 1-phosphate
0.083
NAD+
-
cosubstrate: D-mannnitol 1-phosphate
0.11
NAD+
-
cosubstrate: D-mannitol 1-phosphate
0.2
NAD+
-
cosubstrate: hexitol phosphate
0.38
NAD+
-
cosubstrate: D-mannitol 1-phosphate
0.75
NAD+
pH 7.1, 25°C, recombinant enzyme
0.8
NAD+
-
pH 7.1, 25°C, recombinant enzyme
0.005
NADH

-
cosubstrate: D-fructose 6-phosphate
0.014
NADH
-
pH 10.0, 25°C, recombinant enzyme
0.016
NADH
-
cosubstrate: D-fructose 6-phosphate
0.016
NADH
pH 7.1, 25°C, recombinant enzyme
0.03
NADH
-
higher affinity for NADH
0.099
NADH
-
cosubstrate: D-fructose 6-phosphate
0.14
NADH
-
cosubstrate: D-fructose 6-phosphate
5.4
NADH
-
pH 7.0, 25°C, presence of 200 mM NaCl
16
NADH
recombinant enzyme, pH 7.0, 30°C
56.9
NADH
-
pH 7.0, 25°C, presence of 200 mM NaCl
additional information
additional information

Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
kinetic isotope effects, overview
-
additional information
additional information
-
kinetic isotope effects, overview
-
additional information
additional information
-
steady-state kinetics and kinetic mechanism, binding of D-mannitol 1-phosphate and NAD+ is random, whereas D-fructose 6-phosphate binds only after NADH has bound to the enzyme. Hydride transfer is rate-determining for D-mannitol 1-phosphate oxidation by AfM1PDH. AfM1PDH behaves kinetically as a fructose 6-phosphate reductase, overview
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enhanced tolerance to salt stress in transgenic loblolly pine simultaneously expressing two genes encoding mannitol-1-phosphate dehydrogenase and glucitol-6-phosphate dehydrogenase
-
expression in Escherichia coli
expression in transgenic Oryza sativa plants, basmati indica rice, using the Agrobacterium tumefaciens transfection and DNA integration method, leading to increased tolerance to salinity and drought in the transgenic plants compared to wild-type plants by accumulation of mannitol, overview
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gene BbMPD, DNA and amino acid sequence determination and analysis, phylogenetic tree
gene EsM1PDH2 is located at Esi0020_0181, DNA and amino acid sequence determination and analysis, sequence comparison, real-time-PCR expression analysis, expression in Escherichia coli strain BL21(DE3)
gene EsM1PDH3 is located at Esi0080_0017, DNA and amino acid sequence determination and analysis, sequence comparison, real-time-PCR expression analysis, expression in Escherichia coli strain BL21(DE3)
gene EsM1PHD1 is located at Esi0017_0062, DNA and amino acid sequence determination and analysis, sequence comparison, real-time-PCR expression analysis, expression in Escherichia coli strain BL21(DE3)
gene MPDH1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression analysis
gene MPDH1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression analysis, recombinant expression of His-tagged EsM1PDH1 in Escherichia coli
gene MPDH2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression analysis
gene mtlD or ACIAD1672, DNA and amino acid sequence determination and analysis, real-time-PCR expression analysis, expression of His-tagged enzyme
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gene mtlD, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis
gene mtlD, recombinant expression in Beta vulgaris under control of a stress-inducible rd29A promoter, the transgenic plants are more resistant against stress caused by infections of fungi, e.g. Alternaria alternata, Botrytis cinerea, and Cercospora beticola. Expression profiling by semi-quantitative reverse transcription -PCR analysis shows different levels of cold-inducible expression of mtlD in independent T1 transformants in leafs and whole plants. AAccumulation of mannitol in T1 transgenic lines. Phenotypes, overview
gene mtlD, recombinant expression of the enzyme under control of constitutive promoter CaMV35S in Arachis hypogaea cv. GG 20 via transfection and introduction with Agrobacterium tumefaciens strain LBA 4404, genetic transformation and regeneration of peanut from deembryonated cotyledons. All transgenic plants show accumulation of mannitol. Under water-deficit stress, different transgenic lines have significantly different levels of mannitol suggesting multiple mechanisms controlling the activity of the enzyme encoded by the transgene and the level of gene expression
gene mtld1, encoded in the mannitol operon, sequence comparison, real-time PCR expression analysis, expression as His6-tagged protein in Escherichia coli strain SG13009
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heterologous expression in Escherichia coli JM109
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high level overexpression in Escherichia coli strain JM109
overexpression in Escherichia coli
-
overexpression of enzyme in Lactococcus lactis, small amounts of mannitol are formed in growing cells of lactate dehydrogenase deficient or phosphofructokinase-reduced strains, while resting cells of lactate-dehydrogenase deficient strain convert 25% of glucose into mannitol
-
Petunia hybrida (Hook) Vilm. cv. Mitchell is transformed with an Escherichia coli gene encoding mannitol 1-phosphate dehydrogenase. The high-mannitol containing lines are more tolerant of chilling stress than the low mannitol containing transgenic lines and wild-type. In the higher mannitol lines only 0.04% to 0.06% of the total osmotic potential generated from all solutes can be attributed to mannitol, thus its action is more like that of an osmoprotectant rather than an osmoregulator
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recombinant expression in Escherichia coli
-
expression in Escherichia coli

-
expression in Escherichia coli
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Evidence for and characterization of a mannitol cycle in Eimeria tenella
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brenda
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33
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194
221-227
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146
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brenda
Henstra, S.A.; Tolner, B.; Ten Hoeve Duurkens, R.H.; Konings, W.N.; Robillard, G.T.
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178
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39
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-
brenda
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brenda
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Mannitol-1-phosphate dehydrogenase of Escherichia coli
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239
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211
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brenda
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16
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brenda
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21
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D-Mannitol-1-phosphate dehydrogenase from E. coli
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-
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Metabolic engineering of mannitol production in Lactococcus lactis: influence of overexpression of mannitol 1-phosphate dehydrogenase in different genetic backgrounds
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70
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Mannitol-1-phosphate dehydrogenase (MtlD) is required for mannitol and glucitol assimilation in Bacillus subtilis: possible cooperation of mtl and gut operons
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Escherichia coli
-
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Mannitol 1-phosphate metabolism is required for sporulation in planta of the wheat pathogen Stagonospora nodorum
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Parastagonospora nodorum (Q0U6E8), Parastagonospora nodorum
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Enhanced tolerance to salt stress in transgenic loblolly pine simultaneously expressing two genes encoding mannitol-1-phosphate dehydrogenase and glucitol-6-phosphate dehydrogenase
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Characterization of recombinant Aspergillus fumigatus mannitol-1-phosphate 5-dehydrogenase and its application for the stereoselective synthesis of protio and deuterio forms of D-mannitol 1-phosphate
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Increased tolerance to salinity and drought in transgenic indica rice by mannitol accumulation
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Mannitol, a compatible solute synthesized by Acinetobacter baylyi in a two-step pathway including a salt-induced and salt-dependent mannitol-1-phosphate dehydrogenase
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15
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Acinetobacter baylyi
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Krahulec, S.; Armao, G.C.; Klimacek, M.; Nidetzky, B.
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278
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Aspergillus fumigatus
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Molecular cloning and characterization of mannitol-1-phosphate dehydrogenase from Vibrio cholerae
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21
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brenda
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233
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17
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Acinetobacter baylyi (Q6FBP5), Acinetobacter baylyi ATCC 33305 (Q6FBP5)
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Molecular and biochemical characterization of mannitol-1-phosphate dehydrogenase from the model brown alga Ectocarpus sp.
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117
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brenda
Bhauso, T.D.; Radhakrishnan, T.; Kumar, A.; Mishra, G.P.; Dobaria, J.R.; Patel, K.; Rajam, M.V.
Overexpression of bacterial mtlD gene in peanut improves drought tolerance through accumulation of mannitol
ScientificWorldJournal
2014
125967
2014
Escherichia coli (P09424)
brenda
Goudarzi, A.; Jafari, M.; Safaie, N.; Mohammad Jafari, S.
Transgenic sugar beet expressing a bacterial mannitol-1-phosphate dehydrogenase (mtlD) gene shows enhanced resistance to fungal pathogens
Sugar Tech.
18
192-203
2016
Escherichia coli (P09424)
-
brenda